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PLEKHM2 (NM_015164) Human Tagged ORF Clone
CAT＃: RG220299
- TrueORF®
PLEKHM2 (tGFP-tagged) - Human pleckstrin homology domain containing, family M (with RUN domain) member 2 (PLEKHM2)
ORF Plasmid: DDK
"NM_015164" in other vectors (4)
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CNY 11,780.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | SKIP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG220299 representing NM_015164
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTGCTATTCAACAGCAGAGAAGAATCCCAAAGCTTGTTGGTTCCTGCCGCCGCCGCCGCCGCGGC GCACAGCCCGCGGCCTCCTTCCCCGCCGGCCGCGCTCCGGAGCCTCCGGGCCGCGGTGGAGCGAGGGCCC AGGCGAGGCGAGGGCCGGGCGGCGGGCGCCGGGCCCCGCGGCCGGCACGACGGAGCCCCCGTACGGCCGG CGGCAGCGGTTGGCGGCGGCCCCCGGCCCCCGGCGCGGGAAGCGGCGGCGGGGCGGCGGCGGCGGTGGCG GTGGCGGTGGCGGCGACGGTGGCAGCGCCATGGAGCCGGGGGAGGTGAAGGACCGGATCCTGGAGAACAT CTCGCTGTCGGTGAAGAAGTTGCAGAGCTATTTTGCTGCATGTGAGGATGAGATCCCTGCCATCCGGAAC CATGACAAGGTCCTACAGCGTCTGTGTGAGCACCTGGACCACGCCCTGCTGTACGGACTGCAAGACCTCT CCTCTGGCTACTGGGTGCTCGTGGTGCATTTTACTCGGAGAGAGGCCATCAAGCAGATCGAGGTGCTGCA GCACGTGGCCACCAACCTGGGGCGCAGCCGTGCCTGGCTGTACCTGGCCCTCAACGAGAACTCCTTGGAG AGCTACCTGCGGTTGTTCCAGGAGAACCTGGGCCTGCTGCATAAGTACTACGTCAAGAATGCCCTGGTCT GCAGCCACGATCACCTGACGCTCTTCCTGACCTTGGTGTCCGGGCTAGAGTTCATTCGTTTCGAGCTGGA TCTGGATGCCCCTTACCTAGACCTGGCCCCCTACATGCCCGACTACTACAAACCTCAGTACCTGCTGGAC TTTGAAGACCGCCTTCCCAGCTCGGTCCACGGCTCAGACAGTCTGTCCCTCAACTCTTTCAACTCCGTCA CCTCCACCAACCTGGAGTGGGATGACAGTGCGATTGCCCCATCTAGTGAGGATTATGATTTTGGAGATGT GTTTCCAGCAGTGCCGTCTGTACCCAGCACAGACTGGGAAGATGGAGACCTCACAGACACGGTCAGTGGT CCCCGCTCCACAGCCTCCGACCTGACCAGCAGCAAGGCCTCCACCAGGAGCCCCACCCAGCGCCAGAACC CCTTCAACGAGGAGCCGGCAGAGACTGTGTCCTCCTCTGACACCACCCCCGTGCACACCACCTCTCAGGA GAAGGAGGAGGCCCAGGCCCTGGACCCGCCGGATGCCTGCACGGAGCTCGAGGTCATCAGGGTCACCAAG AAGAAGAAAATTGGCAAGAAGAAAAAGAGCAGATCAGATGAGGAGGCAAGTCCACTCCACCCCGCCTGCA GCCAGAAGAAATGTGCCAAGCAGGGGGACGGTGACAGCCGCAACGGCAGCCCAAGCCTTGGGCGGGACTC GCCAGACACTATGCTTGCCTCCCCCCAGGAGGAGGGAGAGGGGCCGAGCAGCACCACGGAGAGCAGCGAG CGCTCCGAGCCGGGCCTGCTGATCCCTGAGATGAAGGACACCTCCATGGAGCGCTTGGGGCAGCCCCTGA GCAAGGTTATCGACCAGCTCAACGGGCAGCTGGACCCCAGCACCTGGTGCTCCCGTGCTGAGCCCCCAGA CCAGTCCTTTCGGACCGGCTCTCCCGGGGATGCCCCGGAGAGGCCGCCGCTTTGCGACTTTAGTGAGGGG CTTTCAGCCCCAATGGACTTCTACCGCTTTACCGTCGAGAGTCCAAGCACTGTTACATCAGGTGGCGGCC ACCATGACCCTGCAGGGCTTGGCCAACCGCTGCATGTTCCTAGTAGCCCTGAGGCTGCTGGCCAAGAAGA AGAGGGAGGAGGAGGAGAGGGACAGACGCCTCGGCCCCTAGAGGATACCACGAGGGAGGCTCAGGAGCTG GAGGCCCAGCTGTCCCTGGTCAGGGAGGGGCCTGTGTCTGAGCCAGAGCCTGGGACCCAGGAGGTTCTCT GCCAGCTCAAGCGAGACCAGCCCAGCCCGTGTCTGAGTAGCGCTGAGGATTCTGGGGTGGATGAGGGACA GGGGAGCCCTTCGGAGATGGTCCATTCCTCGGAGTTCAGAGTAGACAACAATCACCTGCTCCTGCTCATG ATCCACGTGTTCCGAGAAAACGAAGAGCAGCTGTTCAAAATGATCCGGATGAGCACCGGGCACATGGAGG GCAACCTGCAGCTGCTGTACGTGCTGCTCACAGACTGCTATGTCTACCTGCTCCGGAAAGGGGCCACAGA GAAGCCATACCTGGTGGAAGAGGCCGTTTCTTACAATGAACTTGACTATGTGTCGGTTGGCCTTGACCAG CAGACGGTGAAGCTGGTGTGCACCAACCGCAGGAAGCAGTTTCTGCTGGACACGGCTGATGTGGCGCTGG CTGAGTTCTTTTTGGCTTCTTTGAAGTCAGCCATGATCAAAGGCTGTCGAGAACCTCCCTACCCCAGCAT CCTGACGGATGCCACCATGGAGAAGCTGGCACTGGCCAAATTTGTGGCCCAAGAATCGAAGTGTGAGGCA TCTGCTGTCACCGTGCGCTTCTACGGCCTTGTGCACTGGGAGGACCCCACAGACGAGTCCCTGGGCCCCA CGCCCTGCCACTGCTCACCCCCCGAGGGCACCATCACCAAAGAAGGCATGCTGCACTACAAGGCGGGCAC CTCCTACCTGGGCAAGGAACACTGGAAGACGTGCTTCGTGGTGCTCAGCAACGGGATCCTCTACCAGTAC CCGGACCGCACCGACGTCATCCCTCTGCTCTCGGTGAACATGGGGGGGGAGCAGTGCGGTGGCTGCCGGA GAGCCAACACCACGGATCGGCCCCACGCCTTCCAGGTCATTCTCTCCGACCGGCCCTGCCTGGAGCTAAG TGCCGAGAGCGAGGCCGAGATGGCCGAGTGGATGCAGCATCTCTGCCAGGCTGTGTCCAAAGGGGTCATC CCCCAGGGCGTAGCTCCCAGCCCCTGCATACCCTGCTGCCTGGTCCTCACGGATGACCGCCTCTTTACGT GCCATGAGGATTGCCAGACCAGCTTCTTCCGCTCTTTGGGCACAGCCAAGCTGGGCGACATCAGCGCCGT CTCCACCGAGCCGGGCAAGGAGTACTGCGTCTTGGAGTTCTCCCAGGACAGCCAGCAGCTCCTCCCGCCC TGGGTCATCTACCTGAGCTGCACTTCTGAACTGGACCGATTGCTGTCTGCACTGAACTCTGGGTGGAAAA CCATCTATCAGGTGGACCTCCCCCACACGGCGATCCAGGAAGCCTCCAACAAGAAGAAATTCGAGGATGC CTTGAGCCTCATCCACAGCGCCTGGCAGCGGAGCGACAGTCTCTGCCGCGGCCGAGCCTCCCGAGACCCC TGGTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG220299 representing NM_015164
Red=Cloning site Green=Tags(s) MACYSTAEKNPKACWFLPPPPPRRTARGLLPRRPRSGASGPRWSEGPGEARAGRRAPGPAAGTTEPPYGR RQRLAAAPGPRRGKRRRGGGGGGGGGGGDGGSAMEPGEVKDRILENISLSVKKLQSYFAACEDEIPAIRN HDKVLQRLCEHLDHALLYGLQDLSSGYWVLVVHFTRREAIKQIEVLQHVATNLGRSRAWLYLALNENSLE SYLRLFQENLGLLHKYYVKNALVCSHDHLTLFLTLVSGLEFIRFELDLDAPYLDLAPYMPDYYKPQYLLD FEDRLPSSVHGSDSLSLNSFNSVTSTNLEWDDSAIAPSSEDYDFGDVFPAVPSVPSTDWEDGDLTDTVSG PRSTASDLTSSKASTRSPTQRQNPFNEEPAETVSSSDTTPVHTTSQEKEEAQALDPPDACTELEVIRVTK KKKIGKKKKSRSDEEASPLHPACSQKKCAKQGDGDSRNGSPSLGRDSPDTMLASPQEEGEGPSSTTESSE RSEPGLLIPEMKDTSMERLGQPLSKVIDQLNGQLDPSTWCSRAEPPDQSFRTGSPGDAPERPPLCDFSEG LSAPMDFYRFTVESPSTVTSGGGHHDPAGLGQPLHVPSSPEAAGQEEEGGGGEGQTPRPLEDTTREAQEL EAQLSLVREGPVSEPEPGTQEVLCQLKRDQPSPCLSSAEDSGVDEGQGSPSEMVHSSEFRVDNNHLLLLM IHVFRENEEQLFKMIRMSTGHMEGNLQLLYVLLTDCYVYLLRKGATEKPYLVEEAVSYNELDYVSVGLDQ QTVKLVCTNRRKQFLLDTADVALAEFFLASLKSAMIKGCREPPYPSILTDATMEKLALAKFVAQESKCEA SAVTVRFYGLVHWEDPTDESLGPTPCHCSPPEGTITKEGMLHYKAGTSYLGKEHWKTCFVVLSNGILYQY PDRTDVIPLLSVNMGGEQCGGCRRANTTDRPHAFQVILSDRPCLELSAESEAEMAEWMQHLCQAVSKGVI PQGVAPSPCIPCCLVLTDDRLFTCHEDCQTSFFRSLGTAKLGDISAVSTEPGKEYCVLEFSQDSQQLLPP WVIYLSCTSELDRLLSALNSGWKTIYQVDLPHTAIQEASNKKKFEDALSLIHSAWQRSDSLCRGRASRDP WC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015164 |
| ORF Size | 3366 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015164.1, NP_055979.1 |
| RefSeq Size | 4231 bp |
| RefSeq ORF | 3060 bp |
| Locus ID | 23207 |
| UniProt ID | Q8IWE5 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a protein that binds the plus-end directed microtubule motor protein kinesin, together with the lysosomal GTPase Arl8, and is required for lysosomes to distribute away from the microtubule-organizing center. The encoded protein belongs to the multisubunit BLOC-one-related complex that regulates lysosome positioning. It binds a Salmonella effector protein called Salmonella induced filament A and is a critical host determinant in Salmonella pathogenesis. It has a domain architecture consisting of an N-terminal RPIP8, UNC-14, and NESCA (RUN) domain that binds kinesin-1 as well as the lysosomal GTPase Arl8, and a C-terminal pleckstrin homology domain that binds the Salmonella induced filament A effector protein. Naturally occurring mutations in this gene lead to abnormal localization of lysosomes, impaired autophagy flux and are associated with recessive dilated cardiomyopathy and left ventricular noncompaction. [provided by RefSeq, Feb 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220299 | PLEKHM2 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family M (with RUN domain) member 2 (PLEKHM2) |
CNY 7,096.00 |
|
| RC220299L3 | Lenti ORF clone of Human pleckstrin homology domain containing, family M (with RUN domain) member 2 (PLEKHM2), Myc-DDK-tagged |
CNY 12,640.00 |
|
| RC220299L4 | Lenti ORF clone of Human pleckstrin homology domain containing, family M (with RUN domain) member 2 (PLEKHM2), mGFP tagged |
CNY 12,640.00 |
|
| SC316442 | PLEKHM2 (untagged)-Human pleckstrin homology domain containing, family M (with RUN domain) member 2 (PLEKHM2) |
CNY 19,760.00 |
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