PLEKHM2 (NM_015164) Human Tagged ORF Clone
CAT#: RC220299
PLEKHM2 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family M (with RUN domain) member 2 (PLEKHM2)
ORF Plasmid: tGFP
"NM_015164" in other vectors (4)
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CNY 7,096.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | SKIP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC220299 representing NM_015164
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGGGGGAGGTGAAGGACCGGATCCTGGAGAACATCTCGCTGTCGGTGAAGAAGTTGCAGAGCT ATTTTGCTGCATGTGAGGATGAGATCCCTGCCATCCGGAACCATGACAAGGTCCTACAGCGTCTGTGTGA GCACCTGGACCACGCCCTGCTGTACGGACTGCAAGACCTCTCCTCTGGCTACTGGGTGCTCGTGGTGCAT TTTACTCGGAGAGAGGCCATCAAGCAGATCGAGGTGCTGCAGCACGTGGCCACCAACCTGGGGCGCAGCC GTGCCTGGCTGTACCTGGCCCTCAACGAGAACTCCTTGGAGAGCTACCTGCGGTTGTTCCAGGAGAACCT GGGCCTGCTGCATAAGTACTACGTCAAGAATGCCCTGGTCTGCAGCCACGATCACCTGACGCTCTTCCTG ACCTTGGTGTCCGGGCTAGAGTTCATTCGTTTCGAGCTGGATCTGGATGCCCCTTACCTAGACCTGGCCC CCTACATGCCCGACTACTACAAACCTCAGTACCTGCTGGACTTTGAAGACCGCCTTCCCAGCTCGGTCCA CGGCTCAGACAGTCTGTCCCTCAACTCTTTCAACTCCGTCACCTCCACCAACCTGGAGTGGGATGACAGT GCGATTGCCCCATCTAGTGAGGATTATGATTTTGGAGATGTGTTTCCAGCAGTGCCGTCTGTACCCAGCA CAGACTGGGAAGATGGAGACCTCACAGACACGGTCAGTGGTCCCCGCTCCACAGCCTCCGACCTGACCAG CAGCAAGGCCTCCACCAGGAGCCCCACCCAGCGCCAGAACCCCTTCAACGAGGAGCCGGCAGAGACTGTG TCCTCCTCTGACACCACCCCCGTGCACACCACCTCTCAGGAGAAGGAGGAGGCCCAGGCCCTGGACCCGC CGGATGCCTGCACGGAGCTCGAGGTCATCAGGGTCACCAAGAAGAAGAAAATTGGCAAGAAGAAAAAGAG CAGATCAGATGAGGAGGCAAGTCCACTCCACCCCGCCTGCAGCCAGAAGAAATGTGCCAAGCAGGGGGAC GGTGACAGCCGCAACGGCAGCCCAAGCCTTGGGCGGGACTCGCCAGACACTATGCTTGCCTCCCCCCAGG AGGAGGGAGAGGGGCCGAGCAGCACCACGGAGAGCAGCGAGCGCTCCGAGCCGGGCCTGCTGATCCCTGA GATGAAGGACACCTCCATGGAGCGCTTGGGGCAGCCCCTGAGCAAGGTTATCGACCAGCTCAACGGGCAG CTGGACCCCAGCACCTGGTGCTCCCGTGCTGAGCCCCCAGACCAGTCCTTTCGGACCGGCTCTCCCGGGG ATGCCCCGGAGAGGCCGCCGCTTTGCGACTTTAGTGAGGGGCTTTCAGCCCCAATGGACTTCTACCGCTT TACCGTCGAGAGTCCAAGCACTGTTACATCAGGTGGCGGCCACCATGACCCTGCAGGGCTTGGCCAACCG CTGCATGTTCCTAGTAGCCCTGAGGCTGCTGGCCAAGAAGAAGAGGGAGGAGGAGGAGAGGGACAGACGC CTCGGCCCCTAGAGGATACCACGAGGGAGGCTCAGGAGCTGGAGGCCCAGCTGTCCCTGGTCAGGGAGGG GCCTGTGTCTGAGCCAGAGCCTGGGACCCAGGAGGTTCTCTGCCAGCTCAAGCGAGACCAGCCCAGCCCG TGTCTGAGTAGCGCTGAGGATTCTGGGGTGGATGAGGGACAGGGGAGCCCTTCGGAGATGGTCCATTCCT CGGAGTTCAGAGTAGACAACAATCACCTGCTCCTGCTCATGATCCACGTGTTCCGAGAAAACGAAGAGCA GCTGTTCAAAATGATCCGGATGAGCACCGGGCACATGGAGGGCAACCTGCAGCTGCTGTACGTGCTGCTC ACAGACTGCTATGTCTACCTGCTCCGGAAAGGGGCCACAGAGAAGCCATACCTGGTGGAAGAGGCCGTTT CTTACAATGAACTTGACTATGTGTCGGTTGGCCTTGACCAGCAGACGGTGAAGCTGGTGTGCACCAACCG CAGGAAGCAGTTTCTGCTGGACACGGCTGATGTGGCGCTGGCTGAGTTCTTTTTGGCTTCTTTGAAGTCA GCCATGATCAAAGGCTGTCGAGAACCTCCCTACCCCAGCATCCTGACGGATGCCACCATGGAGAAGCTGG CACTGGCCAAATTTGTGGCCCAAGAATCGAAGTGTGAGGCATCTGCTGTCACCGTGCGCTTCTACGGCCT TGTGCACTGGGAGGACCCCACAGACGAGTCCCTGGGCCCCACGCCCTGCCACTGCTCACCCCCCGAGGGC ACCATCACCAAAGAAGGCATGCTGCACTACAAGGCGGGCACCTCCTACCTGGGCAAGGAACACTGGAAGA CGTGCTTCGTGGTGCTCAGCAACGGGATCCTCTACCAGTACCCGGACCGCACCGACGTCATCCCTCTGCT CTCGGTGAACATGGGGGGGGAGCAGTGCGGTGGCTGCCGGAGAGCCAACACCACGGATCGGCCCCACGCC TTCCAGGTCATTCTCTCCGACCGGCCCTGCCTGGAGCTAAGTGCCGAGAGCGAGGCCGAGATGGCCGAGT GGATGCAGCATCTCTGCCAGGCTGTGTCCAAAGGGGTCATCCCCCAGGGCGTAGCTCCCAGCCCCTGCAT ACCCTGCTGCCTGGTCCTCACGGATGACCGCCTCTTTACGTGCCATGAGGATTGCCAGACCAGCTTCTTC CGCTCTTTGGGCACAGCCAAGCTGGGCGACATCAGCGCCGTCTCCACCGAGCCGGGCAAGGAGTACTGCG TCTTGGAGTTCTCCCAGGACAGCCAGCAGCTCCTCCCGCCCTGGGTCATCTACCTGAGCTGCACTTCTGA ACTGGACCGATTGCTGTCTGCACTGAACTCTGGGTGGAAAACCATCTATCAGGTGGACCTCCCCCACACG GCGATCCAGGAAGCCTCCAACAAGAAGAAATTCGAGGATGCCTTGAGCCTCATCCACAGCGCCTGGCAGC GGAGCGACAGTCTCTGCCGCGGCCGAGCCTCCCGAGACCCCTGGTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220299 representing NM_015164
Red=Cloning site Green=Tags(s) MEPGEVKDRILENISLSVKKLQSYFAACEDEIPAIRNHDKVLQRLCEHLDHALLYGLQDLSSGYWVLVVH FTRREAIKQIEVLQHVATNLGRSRAWLYLALNENSLESYLRLFQENLGLLHKYYVKNALVCSHDHLTLFL TLVSGLEFIRFELDLDAPYLDLAPYMPDYYKPQYLLDFEDRLPSSVHGSDSLSLNSFNSVTSTNLEWDDS AIAPSSEDYDFGDVFPAVPSVPSTDWEDGDLTDTVSGPRSTASDLTSSKASTRSPTQRQNPFNEEPAETV SSSDTTPVHTTSQEKEEAQALDPPDACTELEVIRVTKKKKIGKKKKSRSDEEASPLHPACSQKKCAKQGD GDSRNGSPSLGRDSPDTMLASPQEEGEGPSSTTESSERSEPGLLIPEMKDTSMERLGQPLSKVIDQLNGQ LDPSTWCSRAEPPDQSFRTGSPGDAPERPPLCDFSEGLSAPMDFYRFTVESPSTVTSGGGHHDPAGLGQP LHVPSSPEAAGQEEEGGGGEGQTPRPLEDTTREAQELEAQLSLVREGPVSEPEPGTQEVLCQLKRDQPSP CLSSAEDSGVDEGQGSPSEMVHSSEFRVDNNHLLLLMIHVFRENEEQLFKMIRMSTGHMEGNLQLLYVLL TDCYVYLLRKGATEKPYLVEEAVSYNELDYVSVGLDQQTVKLVCTNRRKQFLLDTADVALAEFFLASLKS AMIKGCREPPYPSILTDATMEKLALAKFVAQESKCEASAVTVRFYGLVHWEDPTDESLGPTPCHCSPPEG TITKEGMLHYKAGTSYLGKEHWKTCFVVLSNGILYQYPDRTDVIPLLSVNMGGEQCGGCRRANTTDRPHA FQVILSDRPCLELSAESEAEMAEWMQHLCQAVSKGVIPQGVAPSPCIPCCLVLTDDRLFTCHEDCQTSFF RSLGTAKLGDISAVSTEPGKEYCVLEFSQDSQQLLPPWVIYLSCTSELDRLLSALNSGWKTIYQVDLPHT AIQEASNKKKFEDALSLIHSAWQRSDSLCRGRASRDPWC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015164 |
ORF Size | 3057 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015164.4 |
RefSeq Size | 4231 bp |
RefSeq ORF | 3060 bp |
Locus ID | 23207 |
UniProt ID | Q8IWE5 |
Protein Families | Druggable Genome |
MW | 112.6 kDa |
Gene Summary | This gene encodes a protein that binds the plus-end directed microtubule motor protein kinesin, together with the lysosomal GTPase Arl8, and is required for lysosomes to distribute away from the microtubule-organizing center. The encoded protein belongs to the multisubunit BLOC-one-related complex that regulates lysosome positioning. It binds a Salmonella effector protein called Salmonella induced filament A and is a critical host determinant in Salmonella pathogenesis. It has a domain architecture consisting of an N-terminal RPIP8, UNC-14, and NESCA (RUN) domain that binds kinesin-1 as well as the lysosomal GTPase Arl8, and a C-terminal pleckstrin homology domain that binds the Salmonella induced filament A effector protein. Naturally occurring mutations in this gene lead to abnormal localization of lysosomes, impaired autophagy flux and are associated with recessive dilated cardiomyopathy and left ventricular noncompaction. [provided by RefSeq, Feb 2017] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC220299L3 | Lenti ORF clone of Human pleckstrin homology domain containing, family M (with RUN domain) member 2 (PLEKHM2), Myc-DDK-tagged |
CNY 12,640.00 |
|
RC220299L4 | Lenti ORF clone of Human pleckstrin homology domain containing, family M (with RUN domain) member 2 (PLEKHM2), mGFP tagged |
CNY 12,640.00 |
|
RG220299 | PLEKHM2 (tGFP-tagged) - Human pleckstrin homology domain containing, family M (with RUN domain) member 2 (PLEKHM2) |
CNY 11,780.00 |
|
SC316442 | PLEKHM2 (untagged)-Human pleckstrin homology domain containing, family M (with RUN domain) member 2 (PLEKHM2) |
CNY 19,760.00 |
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