C3orf37 (HMCES) (NM_001006109) Human Tagged ORF Clone
CAT#: RG208710
- TrueORF®
C3orf37 (tGFP-tagged) - Human chromosome 3 open reading frame 37 (C3orf37), transcript variant 1
ORF Plasmid: DDK
"NM_001006109" in other vectors (6)
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CNY 5,256.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | C3orf37; DC12; SRAPD1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG208710 representing NM_001006109
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTGGGCGAACATCCTGTCACTTACCTAGAGATGTTCTCACGAGAGCTTGCGCCTACCAGGATCGGC GGGGCCAGCAGCGGCTCCCGGAGTGGAGGGACCCTGATAAGTACTGCCCCTCTTACAACAAGAGTCCTCA ATCCAACAGCCCAGTGCTTCTGTCTCGACTGCACTTTGAGAAGGATGCAGACTCATCTGAGCGTATCATT GCTCCCATGCGCTGGGGCTTGGTCCCTTCTTGGTTCAAAGAAAGTGATCCTTCCAAGCTGCAGTTCAATA CTACCAACTGTCGTAGTGATACCGTAATGGAGAAACGGTCATTTAAGGTGCCTCTGGGAAAGGGAAGACG CTGTGTCGTTTTAGCAGATGGATTCTATGAGTGGCAGCGATGTCAGGGAACAAACCAGAGGCAGCCATAC TTCATCTATTTTCCTCAAATCAAGACAGAGAAGTCAGGTAGCATTGGTGCTGCAGATAGTCCTGAGAACT GGGAGAAAGTCTGGGACAACTGGAGGCTGCTGACAATGGCCGGGATCTTTGACTGCTGGGAGCCCCCAGA GGGAGGAGATGTCCTGTATTCCTATACCATCATCACAGTGGATTCCTGCAAAGGCTTGAGTGACATCCAC CACAGGATGCCTGCCATATTAGATGGAGAGGAGGCAGTTTCTAAATGGCTTGACTTTGGTGAAGTCTCAA CTCAGGAAGCTCTGAAATTAATCCACCCAACAGAGAACATCACCTTCCATGCAGTCTCTTCTGTGGTGAA CAACTCGCGAAACAACACTCCTGAGTGTCTGGCTCCTGTCGACTTGGTGGTCAAAAAGGAGCTCAGGGCA AGTGGCAGTAGCCAGAGGATGTTGCAGTGGTTGGCCACAAAGTCACCCAAAAAGGAAGACTCAAAAACAC CTCAAAAGGAAGAGTCAGATGTTCCCCAGTGGTCCAGTCAGTTCCTGCAGAAGAGTCCACTCCCCACCAA GAGAGGCACTGCAGGACTCCTAGAGCAATGGCTGAAGCGGGAGAAGGAGGAGGAACCTGTGGCCAAGCGT CCTTACAGCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208710 representing NM_001006109
Red=Cloning site Green=Tags(s) MCGRTSCHLPRDVLTRACAYQDRRGQQRLPEWRDPDKYCPSYNKSPQSNSPVLLSRLHFEKDADSSERII APMRWGLVPSWFKESDPSKLQFNTTNCRSDTVMEKRSFKVPLGKGRRCVVLADGFYEWQRCQGTNQRQPY FIYFPQIKTEKSGSIGAADSPENWEKVWDNWRLLTMAGIFDCWEPPEGGDVLYSYTIITVDSCKGLSDIH HRMPAILDGEEAVSKWLDFGEVSTQEALKLIHPTENITFHAVSSVVNNSRNNTPECLAPVDLVVKKELRA SGSSQRMLQWLATKSPKKEDSKTPQKEESDVPQWSSQFLQKSPLPTKRGTAGLLEQWLKREKEEEPVAKR PYSQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001006109 |
ORF Size | 1062 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001006109.1, NP_001006109.1 |
RefSeq Size | 1809 bp |
RefSeq ORF | 1065 bp |
Locus ID | 56941 |
UniProt ID | Q96FZ2 |
Gene Summary | Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites (PubMed:30554877). Acts as an enzyme that recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA (PubMed:30554877). The HMCES DNA-protein cross-link is then degraded by the proteasome (PubMed:30554877). Promotes error-free repair of abasic sites by acting as a 'suicide' enzyme that is degraded, thereby protecting abasic sites from translesion synthesis (TLS) polymerases and endonucleases that are error-prone and would generate mutations and double-strand breaks (PubMed:30554877). Acts as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine (By similarity). Specifically binds 5-hydroxymethylcytosine (5hmC)-containing DNA in stem cells (By similarity). May act as an endonuclease that specifically cleaves 5hmC-containing DNA; additional experiments are however required to confirm this activity in vivo (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC208710 | C3orf37 (Myc-DDK-tagged)-Human chromosome 3 open reading frame 37 (C3orf37), transcript variant 1 |
CNY 3,656.00 |
|
RC208710L1 | Lenti ORF clone of Human chromosome 3 open reading frame 37 (C3orf37), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC208710L2 | Lenti ORF clone of Human chromosome 3 open reading frame 37 (C3orf37), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC208710L3 | Lenti ORF clone of Human chromosome 3 open reading frame 37 (C3orf37), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC208710L4 | Lenti ORF clone of Human chromosome 3 open reading frame 37 (C3orf37), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
SC322022 | C3orf37 (untagged)-Human chromosome 3 open reading frame 37 (C3orf37), transcript variant 1 |
CNY 3,656.00 |
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