NBR1 (NM_001291571) Human Tagged ORF Clone
CAT#: RC239803
- TrueORF®
NBR1 (myc-DDK-tagged) - Human neighbor of BRCA1 gene 1 (NBR1), transcript variant 4
ORF Plasmid: tGFP
"NM_001291571" in other vectors (2)
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CNY 9,310.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1A1-3B; IAI3B; M17S2; MIG19 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC239803 representing NM_001291571
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCACAGGTTACTCTAAATGTGACTTTTAAAAATGAAATTCAAAGCTTTCTGGTTTCTGATCCAG AAAATACAACTTGGGCTGATATCGAAGCTATGGTAAAAGTTTCATTTGATCTGAATACTATTCAAATAAA ATACCTGGATGAGGAAAATGAAGAGGTATCCATCAACAGTCAAGGAGAATATGAAGAAGCGCTTAAGATG GCAGTTAAACAGGGAAACCAACTGCAGATGCAAGTCCACGAAGGGCACCATGTCGTTGATGAAGCCCCAC CCCCAGTTGTAGGAGCAAAACGACTAGCTGCCAGGGCAGGGAAGAAGCCACTTGCACATTACTCTTCACT GGTGAGAGTCTTGGGATCAGACATGAAGACCCCAGAGGATCCTGCAGTGCAGTCGTTTCCACTTGTTCCA TGTGACACAGACCAGCCTCAAGACAAGCCCCCAGACTGGTTCACAAGCTACCTGGAGACGTTCAGAGAAC AAGTGGTTAACGAAACGGTTGAGAAGCTTGAACAGAAATTACATGAAAAGCTTGTCCTCCAGAACCCATC CTTGGGTTCTTGTCCCTCAGAAGTCTCAATGCCTACTTCAGAAGAAACATTGTTTTTGCCAGAAAACCAG TTCAGCTGGCATATTGCTTGCAACAACTGCCAAAGAAGGATTGTTGGTGTCCGCTACCAGTGTAGCCTAT GCCCATCCTACAATATCTGTGAAGATTGTGAAGCAGGGCCATATGGCCATGACACTAACCACGTCCTGCT GAAGTTGCGGAGACCTGTTGTGGGCTCCTCTGAACCGTTCTGTCACTCAAAGTACTCTACTCCTCGTCTT CCTGCTGCTCTGGAACAAGTCAGGCTCCAGAAACAGGTTGATAAGAACTTTCTTAAAGCAGAAAAGCAAA GGTTGCGAGCTGAGAAGAAACAACGTAAAGCAGAGGTCAAGGAACTTAAAAAGCAGCTTAAACTCCATAG GAAAATTCACCTGTGGAATTCAATCCATGGACTCCAGAGCCCCAAGTCTCCTTTAGGCCGACCTGAGAGC TTGCTCCAGTCTAATACCCTGATGCTCCCTTTGCAGCCCTGTACCTCCGTTATGCCAATGCTCAGTGCAG CATTTGTGGATGAGAATTTGCCTGATGGGACTCACCTTCAGCCAGGAACCAAGTTTATCAAACACTGGAG GATGAAAAATACAGGAAATGTAAAGTGGAGTGCAGACACAAAGCTCAAGTTCATGTGGGGAAACCTGACT TTGGCTTCCACAGAAAAGAAGGATGTTTTGGTTCCCTGCCTCAAGGCCGGCCATGTGGGAGTTGTATCTG TGGAGTTCATTGCCCCAGCCTTGGAGGGAACGTATACTTCCCATTGGCGTCTTTCTCACAAAGGCCAGCA ATTTGGGCCTCGGGTCTGGTGCAGTATCATAGTAGATCCTTTCCCCTCCGAAGAGAGCCCTGATAACATT GAAAAGGGCATGATCAGCTCAAGCAAAACTGATGATCTCACCTGCCAGCAAGAGGAAACTTTTCTTCTGG CTAAAGAAGAAAGACAGCTTGGTGAAGTGACTGAGCAGACAGAAGGGACAGCAGCCTGCATCCCACAGAA GGCAAAAAATGTTGCCAGTGAGAGGGAGCTCTACATCCCATCTGTGGATCTTCTGACTGCCCAGGACCTG CTGTCCTTTGAGCTGTTGGATATAAACATTGTTCAAGAGTTGGAGAGAGTGCCCCACAACACCCCTGTGG ATGTGACTCCCTGCATGTCTCCTCTGCCACATGACAGTCCTTTAATAGAGAAGCCAGGCTTGGGGCAGAT AGAGGAAGAGAATGAAGGGGCAGGATTTAAAGCACTTCCTGATTCTATGGTGTCAGTAAAGAGGAAGGCT GAGAACATTGCTTCTGTGGAGGAAGCAGAAGAAGACCTGAGTGGGACCCAGTTTGTGTGTGAGACAGTAA TCCGATCCCTTACCTTGGATGCTGCCCCAGACCACAACCCTCCTTGCAGACAGAAGTCCTTGCAGATGAC ATTTGCCTTGCCTGAAGGACCACTTGGAAATGAGAAGGAGGAGATTATCCATATCGCTGAGGAAGAAGCT GTCATGGAGGAGGAGGAGGATGAGGAGGATGAGGAGGAGGAGGATGAGCTCAAAGATGAAGTTCAAAGTC AGTCCTCTGCTTCCTCAGAGGATTACATCATCATCCTGCCTGAGTGCTTTGATACCAGCCGCCCCCTGGG GGATTCTATGTACAGCTCTGCGCTCTCACAGCCAGGCCTGGAGCGAGGTGCTGAAGGCAAGCCTGGGGTT GAGGCTGGGCAGGAACCAGCTGAGGCTGGGGAAAGACTCCCTGGAGGGGAGAACCAGCCACAGGAGCACA GCATAAGTGACATCCTCACGACCTCACAGACTCTGGAAACAGTGCCCCTAATCCCAGAGGTAGTGGAGCT TCCACCGTCACTGCCCAGGAGCTCTCCTTGTGTACATCATCATGGTTCCCCAGGAGTGGATTTACCAGTT ACCATACCAGAAGTTTCTTCAGTCCCTGATCAGATCAGAGGAGAGCCCAGAGGCTCATCAGGACTTGTAA ACAGCAGACAGAAGAGCTATGACCACTCAAGGCACCATCATGGGAGCAGCATTGCTGGAGGACTGGTGAA GGGGGCTTTGTCTGTTGCTGCCTCTGCATACAAGGCCCTGTTTGCTGGGCCACCAGTCACTGCACAGGGT CTCTGGGGCTTGCTGTCATTCCTTCATCTGGCCAAAAAATGCTTCTTCCTGAAAGCCCCGAGTGAAGCAT TCTCCTGGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC239803 representing NM_001291571
Red=Cloning site Green=Tags(s) MEPQVTLNVTFKNEIQSFLVSDPENTTWADIEAMVKVSFDLNTIQIKYLDEENEEVSINSQGEYEEALKM AVKQGNQLQMQVHEGHHVVDEAPPPVVGAKRLAARAGKKPLAHYSSLVRVLGSDMKTPEDPAVQSFPLVP CDTDQPQDKPPDWFTSYLETFREQVVNETVEKLEQKLHEKLVLQNPSLGSCPSEVSMPTSEETLFLPENQ FSWHIACNNCQRRIVGVRYQCSLCPSYNICEDCEAGPYGHDTNHVLLKLRRPVVGSSEPFCHSKYSTPRL PAALEQVRLQKQVDKNFLKAEKQRLRAEKKQRKAEVKELKKQLKLHRKIHLWNSIHGLQSPKSPLGRPES LLQSNTLMLPLQPCTSVMPMLSAAFVDENLPDGTHLQPGTKFIKHWRMKNTGNVKWSADTKLKFMWGNLT LASTEKKDVLVPCLKAGHVGVVSVEFIAPALEGTYTSHWRLSHKGQQFGPRVWCSIIVDPFPSEESPDNI EKGMISSSKTDDLTCQQEETFLLAKEERQLGEVTEQTEGTAACIPQKAKNVASERELYIPSVDLLTAQDL LSFELLDINIVQELERVPHNTPVDVTPCMSPLPHDSPLIEKPGLGQIEEENEGAGFKALPDSMVSVKRKA ENIASVEEAEEDLSGTQFVCETVIRSLTLDAAPDHNPPCRQKSLQMTFALPEGPLGNEKEEIIHIAEEEA VMEEEEDEEDEEEEDELKDEVQSQSSASSEDYIIILPECFDTSRPLGDSMYSSALSQPGLERGAEGKPGV EAGQEPAEAGERLPGGENQPQEHSISDILTTSQTLETVPLIPEVVELPPSLPRSSPCVHHHGSPGVDLPV TIPEVSSVPDQIRGEPRGSSGLVNSRQKSYDHSRHHHGSSIAGGLVKGALSVAASAYKALFAGPPVTAQG LWGLLSFLHLAKKCFFLKAPSEAFSWF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001291571 |
ORF Size | 2811 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001291571.2 |
RefSeq Size | 3387 bp |
RefSeq ORF | 2814 bp |
Locus ID | 4077 |
UniProt ID | Q14596 |
Protein Families | Druggable Genome |
MW | 104.3 kDa |
Gene Summary | The protein encoded by this gene was originally identified as an ovarian tumor antigen monitored in ovarian cancer. The encoded protein contains a B-box/coiled-coil motif, which is present in many genes with transformation potential. It functions as a specific autophagy receptor for the selective autophagic degradation of peroxisomes by forming intracellular inclusions with ubiquitylated autophagic substrates. This gene is located on a region of chromosome 17q21.1 that is in close proximity to the BRCA1 tumor suppressor gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
Documents
Product Manuals |
FAQs |
SDS |