CORIN (NM_001278586) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ATC2; CRN; Lrp4; PEE5; TMPRSS10
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239443 representing NM_001278586 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAACAGTCTCCTGCCCTCGCTCCGGAAGAGCGCTGCCGCAGAGCCGGGTCCCCAAAGCCGGTCTTGA GAGCTGATGACAATAACATGGGCAATGGCTGCTCTCAGAAGCTGGCGACTGCTAACCTCCTCCGGTTCCT ATTGCTGGTCCTGATTCCATGTATCTGTGCTCTCGTTCTCTTGCTGGTGATCCTGCTTTCCTATGTTGGA ACATTACAAAAGGTCTATTTTAAATCAAATGGGAGTGAACCTTTGGTCACTGATGGTGAAATCCAAGGGT CCGATGTTATTCTTACAAATACAATTTATAACCAGAGCACTGTGGTGTCTACTGCACATCCCGACCAACA CGTTCCAGCCTGGACTACGGATGCTTCTCTCCCAGGGGACCAAAGTCACAGGAATACAAGTGCCTGTATG AACATCACCCACAGCCAGTGTCAGATGCTGCCCTACCACGCCACGCTGACACCTCTCCTCTCAGTTGTCA GAAACATGGAAATGGAAAAGTTCCTCAAGTTTTTCACATATCTCCATCGCCTCAGTTGCTATCAACATAT CATGCTGTTTGGCTGTACCCTCGCCTTCCCTGAGTGCATCATTGATGGCGATGACAGTCATGGACTCCTG CCCTGTAGGTCCTTCTGTGAGGCTGCAAAAGAAGGCTGTGAATCAGTCCTGGGGATGGTGAATTACTCCT GGCCGGATTTCCTCAGATGCTCCCAGTTTAGAAACCAAACTGAAAGCAGCAATGTCAGCAGAATTTGCTT CTCACCTCAGCAGGAAAACGGAAAGCAATTGCTCTGTGGAAGGGGTGAGAACTTTCTGTGTGCCAGTGGA ATCTGCATCCCCGGGAAACTGCAATGTAATGGCTACAACGACTGTGACGACTGGAGTGACGAGGCTCATT GCAACTGCAGCGAGAATCTGTTTCACTGTCACACAGGCAAGTGCCTTAATTACAGCCTTGTGTGTGATGG ATATGATGACTGTGGGGATTTGAGTGATGAGCAAAACTGTGCCTGTCACAGCCAGGGTCTGGTGGAATGC AGAAATGGACAATGTATCCCCAGCACGTTTCAATGTGATGGTGACGAGGACTGCAAGGATGGGAGTGATG AGGAGAACTGCAGCGTCATTCAGACTTCATGTCAAGAAGGAGACCAAAGATGCCTCTACAATCCCTGCCT TGATTCATGTGGTGGTAGCTCTCTCTGTGACCCGAACAACAGTCTGAATAACTGTAGTCAATGTGAACCA ATTACATTGGAACTCTGCATGAATTTGCCCTACAACAGTACAAGTTATCCAAATTATTTTGGCCACAGGA CTCAAAAGGAAGCATCCATCAGCTGGGAGTCTTCTCTTTTCCCTGCACTTGTTCAAACCAACTGTTATAA ATACCTCATGTTCTTTTCTTGCACCATTTTGGTACCAAAATGTGATGTGAATACAGGCGAGCATATCCCT CCTTGCAGGGCATTGTGTGAACACTCTAAAGAACGCTGTGAGTCTGTTCTTGGGATTGTGGGCCTACAGT GGCCTGAAGACACAGATTGCAGTCAATTTCCAGAGGAAAATTCAGACAATCAAACCTGCCTGATGCCTGA TGAATATGTGGAAGAATGCTCACCTAGTCATTTCAAGTGCCGCTCAGGACAGTGTGTTCTGGCTTCCAGA AGATGTGATGGCCAGGCCGACTGTGACGATGACAGTGATGAGGAAAACTGTGGTTGTAAAGAGAGAGATC TTTGGGAATGTCCATCCAATAAACAATGTTTGAAGCACACAGTGATCTGCGATGGGTTCCCAGACTGCCC TGATTACATGGACGAGAAAAACTGCTCATTTTGCCAAGATGATGAGCTGGAATGTGCAAACCATGCGTGT GTGTCACGTGACCTGTGGTGTGATGGTGAAGCCGACTGCTCAGACAGTTCAGATGAATGGGACTGTGGTG AGCTACTTGTTTCTGCCATCTGTTGTACTCTCTCATGCCTGTCAGCCACTGAAGCTGAATGGATCCTAAA TTACTTCCTGGATTTTACTGACCAAGTTCATCTATTTTGTTTTACTGTTATAAAAGCTGATAGTGCTGAT CTATGTGTGTTATTTCCAACTCACAGAAAATTTCCCCACGAAGCTACTTCTCTATTTGAAATATCTATAT TGTATGGAAAAACGTTTGGGTTCAAAATGTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239443 representing NM_001278586 Red=Cloning site Green=Tags(s) MKQSPALAPEERCRRAGSPKPVLRADDNNMGNGCSQKLATANLLRFLLLVLIPCICALVLLLVILLSYVG TLQKVYFKSNGSEPLVTDGEIQGSDVILTNTIYNQSTVVSTAHPDQHVPAWTTDASLPGDQSHRNTSACM NITHSQCQMLPYHATLTPLLSVVRNMEMEKFLKFFTYLHRLSCYQHIMLFGCTLAFPECIIDGDDSHGLL PCRSFCEAAKEGCESVLGMVNYSWPDFLRCSQFRNQTESSNVSRICFSPQQENGKQLLCGRGENFLCASG ICIPGKLQCNGYNDCDDWSDEAHCNCSENLFHCHTGKCLNYSLVCDGYDDCGDLSDEQNCACHSQGLVEC RNGQCIPSTFQCDGDEDCKDGSDEENCSVIQTSCQEGDQRCLYNPCLDSCGGSSLCDPNNSLNNCSQCEP ITLELCMNLPYNSTSYPNYFGHRTQKEASISWESSLFPALVQTNCYKYLMFFSCTILVPKCDVNTGEHIP PCRALCEHSKERCESVLGIVGLQWPEDTDCSQFPEENSDNQTCLMPDEYVEECSPSHFKCRSGQCVLASR RCDGQADCDDDSDEENCGCKERDLWECPSNKQCLKHTVICDGFPDCPDYMDEKNCSFCQDDELECANHAC VSRDLWCDGEADCSDSSDEWDCGELLVSAICCTLSCLSATEAEWILNYFLDFTDQVHLFCFTVIKADSAD LCVLFPTHRKFPHEATSLFEISILYGKTFGFKML TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278586
ORF Size	2202 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278586.2
RefSeq Size	2442 bp
RefSeq ORF	2205 bp
Locus ID	10699
Protein Families	Druggable Genome, Protease, Transmembrane
MW	82.3 kDa
Gene Summary	This gene encodes a member of the type II transmembrane serine protease class of the trypsin superfamily. Members of this family are composed of multiple structurally distinct domains. The encoded protein converts pro-atrial natriuretic peptide to biologically active atrial natriuretic peptide, a cardiac hormone that regulates blood volume and pressure. This protein may also function as a pro-brain-type natriuretic peptide convertase. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

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