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PPP2R2A (NM_001177591) Human Tagged ORF Clone
CAT＃: RC230117
PPP2R2A (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B, alpha (PPP2R2A), transcript variant 2
ORF Plasmid: tGFP
"NM_001177591" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | B55A; B55ALPHA; PR52A; PR55A; PR55alpha |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230117 representing NM_001177591
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCCGAAGTTTTCTCTTCGTTCTATGTTTCATGGAGCTGGAGGAGGGAATGATATTCAGTGGTGTT TTTCTCAGGTGAAAGGAGCAGTAGATGATGATGTAGCAGAAGCAGATATAATTTCTACAGTAGAATTTAA TCATTCTGGAGAATTACTAGCAACAGGAGATAAAGGTGGTAGAGTTGTCATCTTTCAACAGGAGCAGGAG AACAAAATCCAGTCTCATAGCAGAGGAGAATATAATGTTTACAGCACCTTCCAGAGCCATGAACCAGAGT TTGACTACTTGAAAAGTTTAGAAATAGAAGAAAAGATCAACAAAATTAGGTGGTTACCCCAGAAAAATGC TGCTCAGTTTTTATTGTCTACCAATGATAAAACAATAAAATTATGGAAAATCAGTGAAAGGGACAAAAGA CCAGAAGGGTATAACTTGAAAGAGGAGGATGGAAGGTATAGAGATCCTACTACAGTTACTACACTACGAG TGCCAGTCTTTAGGCCTATGGATCTAATGGTTGAGGCCAGTCCACGAAGAATATTTGCCAATGCTCATAC ATATCACATCAACTCAATTTCTATTAATAGTGATTATGAAACATATTTATCTGCAGATGATTTGCGGATT AATCTTTGGCATCTGGAAATTACAGACAGGAGTTTTAACATTGTGGATATCAAGCCTGCCAATATGGAAG AGCTAACAGAGGTGATTACAGCAGCAGAATTTCATCCAAACAGCTGTAACACATTTGTATACAGCAGCAG TAAAGGAACTATTCGGCTATGTGACATGAGGGCATCTGCCCTCTGTGATAGACATTCTAAATTGTTTGAA GAACCTGAAGATCCCAGTAACAGGTCATTTTTTTCCGAAATCATCTCCTCTATTTCGGATGTAAAATTCA GCCATAGTGGTCGATATATGATGACTAGAGACTATTTGTCAGTCAAAATTTGGGACTTAAATATGGAAAA CAGGCCTGTGGAAACATACCAGGTGCATGAATACCTCAGAAGTAAACTCTGTTCACTGTATGAAAATGAC TGCATATTTGACAAATTTGAATGTTGTTGGAATGGATCTGACAGTGTTGTCATGACTGGATCTTACAATA ATTTCTTCAGAATGTTTGACAGAAACACAAAGCGAGACATAACCCTAGAAGCATCGCGGGAAAACAATAA GCCTCGCACAGTTCTGAAGCCTCGCAAAGTCTGTGCAAGTGGCAAGCGAAAGAAAGATGAAATAAGTGTT GACAGCCTAGACTTCAATAAGAAAATCCTTCACACAGCCTGGCACCCCAAGGAAAATATCATTGCCGTAG CTACTACAAACAATCTGTATATATTTCAAGACAAAGTGAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230117 representing NM_001177591
Red=Cloning site Green=Tags(s) MFPKFSLRSMFHGAGGGNDIQWCFSQVKGAVDDDVAEADIISTVEFNHSGELLATGDKGGRVVIFQQEQE NKIQSHSRGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQKNAAQFLLSTNDKTIKLWKISERDKR PEGYNLKEEDGRYRDPTTVTTLRVPVFRPMDLMVEASPRRIFANAHTYHINSISINSDYETYLSADDLRI NLWHLEITDRSFNIVDIKPANMEELTEVITAAEFHPNSCNTFVYSSSKGTIRLCDMRASALCDRHSKLFE EPEDPSNRSFFSEIISSISDVKFSHSGRYMMTRDYLSVKIWDLNMENRPVETYQVHEYLRSKLCSLYEND CIFDKFECCWNGSDSVVMTGSYNNFFRMFDRNTKRDITLEASRENNKPRTVLKPRKVCASGKRKKDEISV DSLDFNKKILHTAWHPKENIIAVATTNNLYIFQDKVN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001177591 |
| ORF Size | 1371 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001177591.2 |
| RefSeq ORF | 1374 bp |
| Locus ID | 5520 |
| UniProt ID | P63151 |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | Tight junction |
| MW | 53.4 kDa |
| Gene Summary | The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230117L3 | Lenti-ORF clone of PPP2R2A (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B, alpha (PPP2R2A), transcript variant 2 |
CNY 6,056.00 |
|
| RC230117L4 | Lenti-ORF clone of PPP2R2A (mGFP-tagged)-Human protein phosphatase 2, regulatory subunit B, alpha (PPP2R2A), transcript variant 2 |
CNY 6,056.00 |
|
| RG230117 | PPP2R2A (tGFP-tagged) - Human protein phosphatase 2, regulatory subunit B, alpha (PPP2R2A), transcript variant 2 |
CNY 4,370.00 |
|
| SC328755 | PPP2R2A (untagged)-Human protein phosphatase 2 regulatory subunit B alpha (PPP2R2A) transcript variant 2 |
CNY 7,320.00 |
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