PPP2R2A (NM_001177591) Human Untagged Clone

CAT#: SC328755

PPP2R2A (untagged)-Human protein phosphatase 2 regulatory subunit B alpha (PPP2R2A) transcript variant 2



  "NM_001177591" in other vectors (4)

CNY 7,320.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit anti-PPP2R2A Polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 2,700.00

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TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms B55A; B55ALPHA; PR52A; PR55A; PR55alpha
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC328755 representing NM_001177591.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTTCCCGAAGTTTTCTCTTCGTTCTATGTTTCATGGAGCTGGAGGAGGGAATGATATTCAGTGGTGT
TTTTCTCAGGTGAAAGGAGCAGTAGATGATGATGTAGCAGAAGCAGATATAATTTCTACAGTAGAATTT
AATCATTCTGGAGAATTACTAGCAACAGGAGATAAAGGTGGTAGAGTTGTCATCTTTCAACAGGAGCAG
GAGAACAAAATCCAGTCTCATAGCAGAGGAGAATATAATGTTTACAGCACCTTCCAGAGCCATGAACCA
GAGTTTGACTACTTGAAAAGTTTAGAAATAGAAGAAAAGATCAACAAAATTAGGTGGTTACCCCAGAAA
AATGCTGCTCAGTTTTTATTGTCTACCAATGATAAAACAATAAAATTATGGAAAATCAGTGAAAGGGAC
AAAAGACCAGAAGGGTATAACTTGAAAGAGGAGGATGGAAGGTATAGAGATCCTACTACAGTTACTACA
CTACGAGTGCCAGTCTTTAGGCCTATGGATCTAATGGTTGAGGCCAGTCCACGAAGAATATTTGCCAAT
GCTCATACATATCACATCAACTCAATTTCTATTAATAGTGATTATGAAACATATTTATCTGCAGATGAT
TTGCGGATTAATCTTTGGCATCTGGAAATTACAGACAGGAGTTTTAACATTGTGGATATCAAGCCTGCC
AATATGGAAGAGCTAACAGAGGTGATTACAGCAGCAGAATTTCATCCAAACAGCTGTAACACATTTGTA
TACAGCAGCAGTAAAGGAACTATTCGGCTATGTGACATGAGGGCATCTGCCCTCTGTGATAGACATTCT
AAATTGTTTGAAGAACCTGAAGATCCCAGTAACAGGTCATTTTTTTCCGAAATCATCTCCTCTATTTCG
GATGTAAAATTCAGCCATAGTGGTCGATATATGATGACTAGAGACTATTTGTCAGTCAAAATTTGGGAC
TTAAATATGGAAAACAGGCCTGTGGAAACATACCAGGTGCATGAATACCTCAGAAGTAAACTCTGTTCA
CTGTATGAAAATGACTGCATATTTGACAAATTTGAATGTTGTTGGAATGGATCTGACAGTGTTGTCATG
ACTGGATCTTACAATAATTTCTTCAGAATGTTTGACAGAAACACAAAGCGAGACATAACCCTAGAAGCA
TCGCGGGAAAACAATAAGCCTCGCACAGTTCTGAAGCCTCGCAAAGTCTGTGCAAGTGGCAAGCGAAAG
AAAGATGAAATAAGTGTTGACAGCCTAGACTTCAATAAGAAAATCCTTCACACAGCCTGGCACCCCAAG
GAAAATATCATTGCCGTAGCTACTACAAACAATCTGTATATATTTCAAGACAAAGTGAATTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001177591
Insert Size 1374 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001177591.1
RefSeq Size 3677 bp
RefSeq ORF 1374 bp
Locus ID 5520
UniProt ID P63151
Protein Families Druggable Genome, Phosphatase
Protein Pathways Tight junction
MW 53 kDa
Gene Summary The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]
Transcript Variant: This variant (2) differs in the 5' terminal exon and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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