PPP2R2A (NM_001177591) Human Untagged Clone
CAT#: SC328755
PPP2R2A (untagged)-Human protein phosphatase 2 regulatory subunit B alpha (PPP2R2A) transcript variant 2
CNY 7,320.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | B55A; B55ALPHA; PR52A; PR55A; PR55alpha |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC328755 representing NM_001177591.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTCCCGAAGTTTTCTCTTCGTTCTATGTTTCATGGAGCTGGAGGAGGGAATGATATTCAGTGGTGT TTTTCTCAGGTGAAAGGAGCAGTAGATGATGATGTAGCAGAAGCAGATATAATTTCTACAGTAGAATTT AATCATTCTGGAGAATTACTAGCAACAGGAGATAAAGGTGGTAGAGTTGTCATCTTTCAACAGGAGCAG GAGAACAAAATCCAGTCTCATAGCAGAGGAGAATATAATGTTTACAGCACCTTCCAGAGCCATGAACCA GAGTTTGACTACTTGAAAAGTTTAGAAATAGAAGAAAAGATCAACAAAATTAGGTGGTTACCCCAGAAA AATGCTGCTCAGTTTTTATTGTCTACCAATGATAAAACAATAAAATTATGGAAAATCAGTGAAAGGGAC AAAAGACCAGAAGGGTATAACTTGAAAGAGGAGGATGGAAGGTATAGAGATCCTACTACAGTTACTACA CTACGAGTGCCAGTCTTTAGGCCTATGGATCTAATGGTTGAGGCCAGTCCACGAAGAATATTTGCCAAT GCTCATACATATCACATCAACTCAATTTCTATTAATAGTGATTATGAAACATATTTATCTGCAGATGAT TTGCGGATTAATCTTTGGCATCTGGAAATTACAGACAGGAGTTTTAACATTGTGGATATCAAGCCTGCC AATATGGAAGAGCTAACAGAGGTGATTACAGCAGCAGAATTTCATCCAAACAGCTGTAACACATTTGTA TACAGCAGCAGTAAAGGAACTATTCGGCTATGTGACATGAGGGCATCTGCCCTCTGTGATAGACATTCT AAATTGTTTGAAGAACCTGAAGATCCCAGTAACAGGTCATTTTTTTCCGAAATCATCTCCTCTATTTCG GATGTAAAATTCAGCCATAGTGGTCGATATATGATGACTAGAGACTATTTGTCAGTCAAAATTTGGGAC TTAAATATGGAAAACAGGCCTGTGGAAACATACCAGGTGCATGAATACCTCAGAAGTAAACTCTGTTCA CTGTATGAAAATGACTGCATATTTGACAAATTTGAATGTTGTTGGAATGGATCTGACAGTGTTGTCATG ACTGGATCTTACAATAATTTCTTCAGAATGTTTGACAGAAACACAAAGCGAGACATAACCCTAGAAGCA TCGCGGGAAAACAATAAGCCTCGCACAGTTCTGAAGCCTCGCAAAGTCTGTGCAAGTGGCAAGCGAAAG AAAGATGAAATAAGTGTTGACAGCCTAGACTTCAATAAGAAAATCCTTCACACAGCCTGGCACCCCAAG GAAAATATCATTGCCGTAGCTACTACAAACAATCTGTATATATTTCAAGACAAAGTGAATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites | SgfI-MluI |
ACCN | NM_001177591 |
Insert Size | 1374 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001177591.1 |
RefSeq Size | 3677 bp |
RefSeq ORF | 1374 bp |
Locus ID | 5520 |
UniProt ID | P63151 |
Protein Families | Druggable Genome, Phosphatase |
Protein Pathways | Tight junction |
MW | 53 kDa |
Gene Summary | The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010] Transcript Variant: This variant (2) differs in the 5' terminal exon and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC230117 | PPP2R2A (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B, alpha (PPP2R2A), transcript variant 2 |
CNY 3,656.00 |
|
RC230117L3 | Lenti-ORF clone of PPP2R2A (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B, alpha (PPP2R2A), transcript variant 2 |
CNY 6,056.00 |
|
RC230117L4 | Lenti-ORF clone of PPP2R2A (mGFP-tagged)-Human protein phosphatase 2, regulatory subunit B, alpha (PPP2R2A), transcript variant 2 |
CNY 6,056.00 |
|
RG230117 | PPP2R2A (tGFP-tagged) - Human protein phosphatase 2, regulatory subunit B, alpha (PPP2R2A), transcript variant 2 |
CNY 4,370.00 |
其它PPP2R2A产品