TFEB (NM_007162) Human Tagged Lenti ORF Clone
CAT#: RC207153L1
- LentiORF®
Lenti ORF clone of Human transcription factor EB (TFEB), transcript variant 1, Myc-DDK-tagged
"NM_007162" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 5,890.00
CNY 600.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ALPHATFEB; BHLHE35; TCFEB |
Vector | pLenti-C-Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC207153).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_007162 |
ORF Size | 1428 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007162.1 |
RefSeq Size | 2364 bp |
RefSeq ORF | 1431 bp |
Locus ID | 7942 |
UniProt ID | P19484 |
Protein Families | Druggable Genome, Transcription Factors |
MW | 52.9 kDa |
Gene Summary | Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Regulates lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1 (PubMed:29146937).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207153 | TFEB (Myc-DDK-tagged)-Human transcription factor EB (TFEB), transcript variant 1 |
CNY 3,656.00 |
|
RC207153L2 | Lenti ORF clone of Human transcription factor EB (TFEB), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
RC207153L3 | Lenti ORF clone of Human transcription factor EB (TFEB), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC207153L4 | Lenti ORF clone of Human transcription factor EB (TFEB), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
RG207153 | TFEB (tGFP-tagged) - Human transcription factor EB (TFEB), transcript variant 1 |
CNY 5,256.00 |
|
SC122773 | TFEB (untagged)-Human transcription factor EB (TFEB), transcript variant 1 |
CNY 5,488.00 |