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TFEB (NM_007162) Human Untagged Clone
CAT＃: SC122773
TFEB (untagged)-Human transcription factor EB (TFEB), transcript variant 1
CNY 5,488.00
| Cited in 3 publications. |
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ALPHATFEB; BHLHE35; TCFEB |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_007162 edited
GGCCGGGCGGGCATGGGCCTTCCCGGCCCGGAGCTGGGAGTCGAAGGGGCGGGAGGCGTG ATGGTGAACTCGCAAGAAGTTTGAGGGACGCGCGGGCCCCGCGCCCACTCCCCCTCCACC GGACACGGCTGGGGCCGGCGATGCCTGAGAGGGGGTCGGAGGACGCAGTGAACATATATG CATGTACAGTGTGGATCCTCATCTGAGAGGAGGGAGATGAAAACACACCCACCTCACAGG CTGTTGTGAGGACTAAGGGTGCGGCAGTGCCTGGTACATGGGAGCCAGCGCCGGCAGCCA CCATGGCGTCACGCATAGGGTTGCGCATGCAGCTCATGCGGGAGCAGGCGCAGCAGGAGG AGCAGCGGGAGCGCATGCAGCAACAGGCTGTCATGCATTACATGCAGCAGCAGCAGCAGC AGCAACAGCAGCAGCTCGGAGGGCCGCCCACCCCGGCCATCAATACCCCCGTCCACTTCC AGTCGCCACCACCTGTGCCTGGGGAGGTGTTGAAGGTGCAGTCCTACCTGGAGAATCCCA CATCCTACCATCTGCAGCAGTCGCAGCATCAGAAGGTGCGGGAGTACCTGTCCGAGACCT ATGGGAACAAGTTTGCTGCCCACATCAGCCCAGCCCAGGGCTCTCCGAAACCCCCACCAG CCGCCTCCCCAGGGGTGCGAGCTGGACACGTGCTGTCCTCCTCCGCTGGCAACAGTGCTC CCAATAGCCCCATGGCCATGCTGCACATTGGCTCCAACCCTGAGAGGGAGTTGGATGATG TCATTGACAACATTATGCGTCTGGACGATGTCCTTGGCTACATCAATCCTGAAATGCAGA TGCCCAACACGCTACCCCTGTCCAGCAGCCACCTGAATGTGTACAGCAGCGACCCCCAGG TCACAGCCTCCCTGGTGGGCGTCACCAGCAGCTCCTGCCCTGCGGACCTGACCCAGAAGC GAGAGCTCACAGATGCTGAGAGCAGGGCCCTGGCCAAGGAGCGGCAGAAGAAAGACAATC ACAACTTAATTGAAAGGAGACGAAGGTTCAACATCAATGACCGCATCAAGGAGTTGGGAA TGCTGATCCCCAAGGCCAATGACCTGGACGTGCGCTGGAACAAGGGCACCATCCTCAAGG CCTCTGTGGATTACATCCGGAGGATGCAGAAGGACCTGCAAAAGTCCAGGGAGCTGGAGA ACCACTCTCGCCGCCTGGAGATGACCAACAAGCAGCTCTGGCTCCGTATCCAGGAGCTGG AGATGCAGGCTCGAGTGCACGGCCTCCCTACCACCTCCCCGTCCGGCATGAACATGGCTG AGCTGGCCCAGCAGGTGGTGAAGCAGGAGCTGCCTAGCGAAGAGGGCCCAGGGGAGGCCC TGATGCTGGGGGCTGAGGTCCCTGACCCTGAGCCACTGCCAGCTCTGCCCCCGCAAGCCC CGCTGCCCCTGCCCACCCAGCCACCATCCCCATTCCATCACCTGGACTTCAGCCACAGCC TGAGCTTTGGGGGCAGGGAGGACGAGGGTCCCCCGGGCTACCCCGAACCCCTGGCGCCGG GGCATGGCTCCCCATTCCCCAGCCTGTCCAAGAAGGATCTGGACCTCATGCTCCTGGACG ACTCACTGCTACCGCTGGCCTCTGATCCACTTCTGTCCACCATGTCCCCCGAGGCCTCCA AGGCCAGCAGCCGCCGGAGCAGCTTCAGCATGGAGGAGGGCGATGTGCTGTGACCCTGGC TGCCCCTGTGCCAGGGAACAGGGGCCGGCCTGGGGGCTGGGAGGGCCAGGGGCACCTCCC TCCCACCCTTCAGGCTGCACTGTGTGTGAAGTAGCCACCTGCCCTGCCTCCCTCCTCCCC GTTGGCCCCTGTTTGGACTTAGTGCCTGTCTGGCAGCCTGTGGGGTCAGGAGAAGCACCC CCAGGGCAGCCCTCTTGACTGGCGCAGTGGGAAGAGGCCTTCAGCCCCTCTCCCGGAGAT GGAATCGCGGGGCAGGGAGGGGCAGGGTGTTCTAGAGGTGAGAAGAGGGCCTGGTGGAGA TTCCCTGTCTTCTGAGCCCGAGCCCCTCATTACCAGTGAAGGACATGCTTGAGGGGTTCG GGAAGCTCCTCATCTGAGGCAACTGGTCCTGGGGGTGCTCAGGCCTGCCTTTTTGGGACT CAGATGGCAGGAGGTCCACCCCGCAGCCTGGTCCTCGGCTCTCCCACAGGTGGGCACCCC CCACTTTGGTGCTAATAGCTCTCCACCAGGTGGTGTGAGCGCGGGGGCTGCCAGAAGCGG GAGGGGTCACTGCCGGAAGAGCAGCTGCCCTCCGACCCCTCACTTTGTGCCTTTAGTAAA CACTGTGCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_007162 unedited
GCGTACAAATTTTGTAATACGACTCACTATAGNNGGCGGCCGCGNAATTCCCGGGATGGC CGGGCGGGCATGGGCCTTCCCGGCCCGGAGCTGGGAGTCGAAGGGGCGGGAGGCGTGATG GTGAACTCGCAAGAAGTTTGAGGGACGCGCGGGCCCCGCGCCCACTCCCCCTCCACCGGA CACGGCTGGGGCCGGCGATGCCTGAGAGGGGGTCGGAGGACGCAGTGAACATATATGCAT GTACAGTGTGGATCCTCATCTGAGAGGAGGGAGATGAAAACACACCCACCTCACAGGCTG TTGTGAGGACTAAGGGTGCGGCAGTGCCTGGTACATGGGAGCCAGCGCCGGCAGCCACCA TGGCGTCACGCATAGGGTTGCGCATGCAGCTCATGCGGGAGCAGGCGCAGCAGGAGGAGC AGCGGGAGCGCATGCAGCAACAGGCTGTCATGCATTACATGCAGCAGCAGCAGCAGCAGC AACAGCAGCAGCTCGGAGGGCCGCCCACCCCGGCCATCAATACCCCCGTCCACTTCCAGT CGCCACCACCTGTGCCTGGGGAGGTGTTGAAGGTGCAGTCCTACCTGGAGAATCCCACAT CCTACCATCTGCAGCAGTCGCAGCATCAGAAGGTGCGGGAGTACCTGTCCGAGACCTATG GGAACAAGTTTGCTGCCCACATCAGCCCAGCCCAAGGCTCTCCGAAACCCCCACAGCCGC CTCCCAGGGGTGCGAGCTGGAACGTGCTGTCCTCCTCCGCTGGCAACAGTGCTCCCAATT ACCCCATGGGCAGGCTGCACATTGGGCTCCAACCTGTAAAGGGAGTGGGATAATGTCATT GGACAACTTATGCCTTCGGGCCAAGGCCCTTGGCTACATCAACCCTGAAAGGCAAATGCC CACCACGCTACCCTGGG |
| Restriction Sites | Please inquire |
| ACCN | NM_007162 |
| Insert Size | 2446 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007162.1, NP_009093.1 |
| RefSeq Size | 2446 bp |
| RefSeq ORF | 1431 bp |
| Locus ID | 7942 |
| UniProt ID | P19484 |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Regulates lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1 (PubMed:29146937).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) contains an alternate 5' sequence, which results in a downstream AUG start codon, compared to variant 2. The resulting isoform (1) has a shorter N-terminus, compared to isoform 2. Variants 1, 3 and 4 encode the same isoform 1. |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Lysosomal dysfunction of corneal fibroblasts underlies the pathogenesis of Granular Corneal Dystrophy Type 2 and can be rescued by TFEB. Lysosomal dysfunction of corneal fibroblasts underlies the pathogenesis of Granular Corneal Dystrophy Type 2 and can be rescued by TFEB
,null,
Journal of Cellular and Molecular Medicine
,PubMed ID 32667742
[TFEB]
|
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TFEB controls vascular development by regulating the proliferation of endothelial cells
,Doronzo, G;Astanina, E;Corà, D;Chiabotto, G;Comunanza, V;Noghero, A;Neri, F;Puliafito, A;Primo, L;Spampanato, C;Settembre, C;Ballabio, A;Camussi, G;Oliviero, S;Bussolino, F;,
EMBO J.
,PubMed ID 30591554
[TFEB]
|
|
PGC-1a Rescues Huntington’s Disease Proteotoxicity by Preventing Oxidative Stress and Promoting TFEB Function
,Taiji Tsunemi, Travis D. Ashe, Bradley E. Morrison, Kathryn R. Soriano, Jonathan Au, Ruben A. Vázquez Roque, Eduardo R. Lazarowski, Vincent A. Damian, Eliezer Masliah, and Albert R. La Spada,
Science Translational Medicine, Jul 2012; 4: 142ra97.
[TFEB]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207153 | TFEB (Myc-DDK-tagged)-Human transcription factor EB (TFEB), transcript variant 1 |
CNY 3,656.00 |
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| RC207153L1 | Lenti ORF clone of Human transcription factor EB (TFEB), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
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| RC207153L2 | Lenti ORF clone of Human transcription factor EB (TFEB), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RC207153L3 | Lenti ORF clone of Human transcription factor EB (TFEB), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC207153L4 | Lenti ORF clone of Human transcription factor EB (TFEB), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG207153 | TFEB (tGFP-tagged) - Human transcription factor EB (TFEB), transcript variant 1 |
CNY 5,256.00 |
