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WHIP (WRNIP1) (NM_130395) Human Tagged ORF Clone
CAT＃: RC202950
WRNIP1 (Myc-DDK-tagged)-Human Werner helicase interacting protein 1 (WRNIP1), transcript variant 2
ORF Plasmid: tGFP
"NM_130395" in other vectors (7)
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CNY 4,768.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | bA420G6.2; CFAP93; FAP93; WHIP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202950 representing NM_130395
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGTGAGCGGGCCGGAAGACGACCCCTTCCTTTCGCAGCTGCACCAGGTGCAGTGCCCCGTGTGCC AGCAGATGATGCCCGCCGCGCACATCAACTCGCACCTGGACCGCTGTCTGCTGCTCCACCCGGCGGGGCA CGCGGAGCCCGCGGCCGGGTCGCACCGCGCCGGGGAGCGGGCCAAGGGGCCCTCGCCGCCCGGCGCCAAG AGGCGGCGGCTGTCGGAGAGCTCGGCGCTGAAGCAGCCAGCCACCCCGACGGCAGCCGAGAGCAGTGAGG GCGAGGGCGAGGAGGGCGACGACGGCGGCGAGACCGAGAGCCGCGAGAGCTACGACGCGCCGCCCACACC CAGCGGCGCCCGCCTTATCCCCGACTTCCCGGTGGCCCGCTCCAGCAGCCCCGGGAGGAAGGGGTCGGGG AAGAGGCCGGCGGCCGCCGCCGCGGCGGGGAGCGCGTCTCCGCGCAGCTGGGACGAGGCGGAGGCGCAGG AGGAGGAGGAGGCCGTGGGCGACGGCGATGGCGACGGGGACGCGGACGCGGACGGCGAGGACGACCCGGG GCACTGGGACGCGGACGCTGCCGAAGCCGCCACCGCCTTCGGGGCCAGCGGCGGGGGCCGCCCGCACCCC CGGGCGCTGGCTGCCGAGGAGATCCGACAGATGCTACAGGGCAAGCCGCTGGCCGACACGATGCGTCCTG ACACGCTGCAGGATTACTTCGGGCAGAGCAAGGCCGTGGGCCAGGATACCCTGCTGCGCTCGCTCCTGGA GACCAACGAAATCCCCTCGCTTATCCTGTGGGGGCCGCCGGGCTGCGGCAAGACCACTCTGGCTCACATC ATAGCCAGCAACAGCAAGAAACATAGCATAAGGTTTGTGACATTATCTGCAACAAATGCCAAGACAAATG ATGTGCGAGATGTCATAAAACAAGCTCAAAATGAAAAGAGCTTTTTCAAAAGGAAAACCATCCTTTTTAT TGATGAGATTCATCGGTTCAATAAATCTCAGCAGGTCAACGCTGCTCTTCTGAGCCGCTGTCGAGTGATT GTTCTTGAGAAGCTTCCAGTAGAGGCAATGGTGACTATTTTAATGCGAGCGATCAACTCCCTGGGAATCC ACGTCCTAGACTCTAGCCGTCCCACTGACCCTCTGAGCCACAGCAGCAACAGCAGCTCAGAGCCCGCCAT GTTCATAGAGGATAAAGCAGTAGACACCCTGGCTTACCTCAGTGACGGTGACGCCCGAGCTGGGTTGAAC GGACTGCAGCTGGCGGTGCTGGCTAGGTTAAGCTCTAGGAAGATGTTCTGTAAGAAGAGTGGGCAATCCT ATTCTCCCAGTAGAGTTCTGATCACAGAGAATGACGTGAAGGAGGGCCTACAGCGATCCCACATTTTATA TGACCGGGCAGGTGAGGAGCATTACAACTGCATCTCCGCCCTGCACAAGTCCATGCGGGGCTCAGACCAG AACGCCTCCCTCTACTGGCTGGCTCGCATGCTCGAGGGAGGAGAGGACCCACTCTACGTGGCACGGAGGC TTGTCAGGTTTGCCAGCGAGGACATAGGTCTGGCAGACCCGTCTGCGTTAACACAAGCGGTTGCTGCCTA CCAAGGCTGTCATTTTATAGGCATGCCTGAATGTGAGGTGCTTCTGGCCCAGTGTGTGGTCTACTTTGCC AGAGCCCCAAAGTCCATTGAGGTGTACAGCGCCTACAACAACGTCAAAGCCTGCCTGAGGAACCACCAGG GGCCACTGCCCCCCGTGCCCCTGCACCTGAGGAACGCGCCCACTAGGCTGATGAAGGATTTGGGCTATGG CAAAGGCTACAAGTACAACCCCATGTACAGCGAGCCTGTGGATCAGGAGTACCTGCCTGAAGAGTTGAGG GGGGTAGATTTCTTCAAGCAGAGGAGGTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202950 representing NM_130395
Red=Cloning site Green=Tags(s) MEVSGPEDDPFLSQLHQVQCPVCQQMMPAAHINSHLDRCLLLHPAGHAEPAAGSHRAGERAKGPSPPGAK RRRLSESSALKQPATPTAAESSEGEGEEGDDGGETESRESYDAPPTPSGARLIPDFPVARSSSPGRKGSG KRPAAAAAAGSASPRSWDEAEAQEEEEAVGDGDGDGDADADGEDDPGHWDADAAEAATAFGASGGGRPHP RALAAEEIRQMLQGKPLADTMRPDTLQDYFGQSKAVGQDTLLRSLLETNEIPSLILWGPPGCGKTTLAHI IASNSKKHSIRFVTLSATNAKTNDVRDVIKQAQNEKSFFKRKTILFIDEIHRFNKSQQVNAALLSRCRVI VLEKLPVEAMVTILMRAINSLGIHVLDSSRPTDPLSHSSNSSSEPAMFIEDKAVDTLAYLSDGDARAGLN GLQLAVLARLSSRKMFCKKSGQSYSPSRVLITENDVKEGLQRSHILYDRAGEEHYNCISALHKSMRGSDQ NASLYWLARMLEGGEDPLYVARRLVRFASEDIGLADPSALTQAVAAYQGCHFIGMPECEVLLAQCVVYFA RAPKSIEVYSAYNNVKACLRNHQGPLPPVPLHLRNAPTRLMKDLGYGKGYKYNPMYSEPVDQEYLPEELR GVDFFKQRRC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_130395 |
| ORF Size | 1920 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_130395.3 |
| RefSeq Size | 2592 bp |
| RefSeq ORF | 1923 bp |
| Locus ID | 56897 |
| UniProt ID | Q96S55 |
| Domains | AAA, AAA, ZnF_Rad18 |
| MW | 69.3 kDa |
| Gene Summary | Werner's syndrome is a rare autosomal recessive disorder characterized by accelerated aging that is caused by defects in the Werner syndrome ATP-dependent helicase gene (WRN). The protein encoded by this gene interacts with the exonuclease-containing N-terminal portion of the Werner protein. This protein has a ubiquitin-binding zinc-finger domain in the N-terminus, an ATPase domain, and two leucine zipper motifs in the C-terminus. It has sequence similarity to replication factor C family proteins and is conserved from E. coli to human. This protein likely accumulates at sites of DNA damage by interacting with polyubiquinated proteins and also binds to DNA polymerase delta and increases the initiation frequency of DNA polymerase delta-mediated DNA synthesis. This protein also interacts with nucleoporins at nuclear pore complexes. Two transcript variants encoding different isoforms have been isolated for this gene. [provided by RefSeq, Jul 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202950L1 | Lenti ORF clone of Human Werner helicase interacting protein 1 (WRNIP1), transcript variant 2, Myc-DDK-tagged |
CNY 7,168.00 |
|
| RC202950L2 | Lenti ORF clone of Human Werner helicase interacting protein 1 (WRNIP1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC202950L3 | Lenti ORF clone of Human Werner helicase interacting protein 1 (WRNIP1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202950L4 | Lenti ORF clone of Human Werner helicase interacting protein 1 (WRNIP1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG202950 | WRNIP1 (tGFP-tagged) - Human Werner helicase interacting protein 1 (WRNIP1), transcript variant 2 |
CNY 4,370.00 |
|
| SC120325 | WRNIP1 (untagged)-Human Werner helicase interacting protein 1 (WRNIP1), transcript variant 2 |
CNY 4,776.00 |
|
| SC320312 | WRNIP1 (untagged)-Human Werner helicase interacting protein 1 (WRNIP1), transcript variant 2 |
CNY 4,776.00 |
