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Phex (NM_011077) Mouse Tagged ORF Clone
CAT＃: MR227127
- TrueORF®
Phex (Myc-DDK-tagged) - Mouse phosphate regulating gene with homologies to endopeptidases on the X chromosome (hypophosphatemia, vitamin D resistant rickets) (Phex)
ORF Plasmid: tGFP
"NM_011077" in other vectors (4)
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CNY 5,488.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Gy; HPDR; HPDR1; Hyp; PEX |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227127 representing NM_011077
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGCAGAAACAGGGAGCACCATGGAGACTGGAAAGGGGACCAACCGAGGCATTCGGATTGCACTGG CCCTGTTTATTGGTGGCACCCTGGTGTTGGGCACACTGCTCTTTCTAGTGAGTCAAGGTCTCCTAAGTTT CCAAGCTAAACAGGAGTACTGTCTGAAGCCAGAATGCATAGAAGCCGCTGCTGCCATCATGAGCAAAGTA AATCTTTCTGTGGATCCTTGTGAGAATTTTTTCCGGTTTGCTTGTGATGGCTGGATAAGCAATAACCCAA TTCCTGAAGATATGCCAAGCTATGGGGTTTATCCTTGGCTGAGACACAATGTTGACCTCAAGTTGAAGGC ACTTCTGGAGAAATCAGTCAGCCGAAGGCGGGACACTGAAGCCGTACAGAAAGCCAAAATCCTCTACTCA TCCTGCATGAATGAGAAAGCAATTGAAAAAGCAGATGCCAAGCCACTGCTCCACATCTTGAGGCATTCAC CTTTCCGCTGGCCAGTGCTTGAAGCTAATATTGGTCCTGAAGGGGTTTGGTCAGAGAGAAAATTCAGTCT ACTGCAAACACTGGCAACGTTCCGCGGTCAATACAGCAATTCTGTGTTCATCCGTTTGTATGTGTCCCCT GATGACAAGGCATCCAATGAACATATCTTGAAGCTGGACCAAGCAACACTCTCTCTGGCTGTAAGGGAAG ACTTCCTGGATAACACTACTGAAGCCAAATCTTATCGGGATGCCCTTTACAAATTCATGGTGGACACTGC TGTGCTTTTAGGAGCTAATAGCTCTCGAGCTGAACATGACATGAAGTCGGTGCTTAGACTGGAAATTAAG ATAGCTGAGATAATGATTCCACATGAGAACCGAACCAGTGAGGCTATGTATAACAAAATGAACATCTCAG AACTCAGCGCTATGATTCCCCAGTTTGACTGGCTGGGCTATATCAAGAAGGTCATTGATACCAGACTCTA CCCACACTTGAAAGACATTGGTCCCTCGGAGAATGTGGTGGTCCGCGTCCCACAGTACTTTAAAGATTTG TTTAGGATATTAGGTGCCGAGAGGAAGAAAACCATTGCCAATTATTTAGTGTGGAGAATGGTTTATTCCA GAATTCCAAACCTCAGCAGGCGCTTTCAATATAGATGGCTAGAATTCTCAAGGGTAATCCAGGGGACCAC AACTCTGCTGCCTCAGTGGGACAAATGTGTCAACTTTATTGAGAGTGCACTCCCATATGTTGTGGGGAAA ATGTTTGTGAATGTTCACTTCCAGGAGGATAAGAAGGAAATGATGGAAGAATTGATTGAGGGTGTTCGCT GGGCCTTCATTGACATGCTGGAGAAAGAAAATGAATGGATGGATGCAGGGACAAAAAGGAAAGCTCAAGA AAAGGCAAGAGCTGTTTTGGCAAAAGTTGGCTATCCAGAGTTTATAATGAATGATACTTATGTTAATGAA GACCTCAAGGCAATCAAATTTTCAGAATCGGACTACTTTGGCAACGTACTGCAAACCCGCAAGTATTTAG CACAGTCTGATTTCTTCTGGCTAAGAAAAGCTGTTCCCAAAACAGAGTGGTTTACAAACCCAACAACAGT CAATGCCTTTTACAGTGCATCTACCAACCAGATACGATTTCCTGCTGGAGAGCTGCAGAAGCCTTTCTTT TGGGGAACAGAATACCCTCGATCCCTGAGTTATGGTGCTATAGGAGTAATTGTGGGCCATGAATTTACAC ATGGATTTGATAATAATGGTAGAAAATATGATAAAAATGGAAACCTTGATCCGTGGTGGTCTGTGGAATC AGAAGAAAAGTTTAAGGAAAAAACAAAATGCATGATTAACCAGTATAGCAACTATTATTGGAAGAAAGCC GGCTTAAATGTGAAAGGGAAGAGGACCCTGGGAGAAAATATTGCTGATAATGGGGGTCTGCGAGAAGCTT TTAGGGCTTACAGGAAATGGATAAATGATAGAAGACAGGGAGTTGAAGAGCCCCTCCTACCAGGCATCAC ATTCACCAACAATCAGCTCTTCTTCCTGAGTTATGCTCATGTAAGGTGCAATTCCTATAGACCAGAAGCT GCCAGAGAACAAGTGCAAATTGGTGCTCACAGTCCTCCACAATTTAGGGTCAATGGTGCCATTAGCAACT TTGAAGAATTTCAGAAAGCTTTTAACTGTCCACGAAATTCCACTATGAACAGAGGTGCAGATTCCTGCCG ACTCTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227127 representing NM_011077
Red=Cloning site Green=Tags(s) MEAETGSTMETGKGTNRGIRIALALFIGGTLVLGTLLFLVSQGLLSFQAKQEYCLKPECIEAAAAIMSKV NLSVDPCENFFRFACDGWISNNPIPEDMPSYGVYPWLRHNVDLKLKALLEKSVSRRRDTEAVQKAKILYS SCMNEKAIEKADAKPLLHILRHSPFRWPVLEANIGPEGVWSERKFSLLQTLATFRGQYSNSVFIRLYVSP DDKASNEHILKLDQATLSLAVREDFLDNTTEAKSYRDALYKFMVDTAVLLGANSSRAEHDMKSVLRLEIK IAEIMIPHENRTSEAMYNKMNISELSAMIPQFDWLGYIKKVIDTRLYPHLKDIGPSENVVVRVPQYFKDL FRILGAERKKTIANYLVWRMVYSRIPNLSRRFQYRWLEFSRVIQGTTTLLPQWDKCVNFIESALPYVVGK MFVNVHFQEDKKEMMEELIEGVRWAFIDMLEKENEWMDAGTKRKAQEKARAVLAKVGYPEFIMNDTYVNE DLKAIKFSESDYFGNVLQTRKYLAQSDFFWLRKAVPKTEWFTNPTTVNAFYSASTNQIRFPAGELQKPFF WGTEYPRSLSYGAIGVIVGHEFTHGFDNNGRKYDKNGNLDPWWSVESEEKFKEKTKCMINQYSNYYWKKA GLNVKGKRTLGENIADNGGLREAFRAYRKWINDRRQGVEEPLLPGITFTNNQLFFLSYAHVRCNSYRPEA AREQVQIGAHSPPQFRVNGAISNFEEFQKAFNCPRNSTMNRGADSCRLW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011077 |
| ORF Size | 2247 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011077.2, NP_035207.1 |
| RefSeq Size | 6265 bp |
| RefSeq ORF | 2250 bp |
| Locus ID | 18675 |
| UniProt ID | P70669 |
| MW | 86.9 kDa |
| Gene Summary | Peptidase that cleaves SIBLING (small integrin-binding ligand, N-linked glycoprotein)-derived ASARM peptides, thus regulating their biological activity (By similarity). Cleaves ASARM peptides between Ser and Glu or Asp residues (By similarity). Regulates osteogenic cell differentiation and bone mineralization through the cleavage of the MEPE-derived ASARM peptide (PubMed:11159866, PubMed:18597632, PubMed:26051469). Promotes dentin mineralization and renal phosphate reabsorption by cleaving DMP1- and MEPE-derived ASARM peptides (PubMed:26051469). Inhibits the cleavage of MEPE by CTSB/cathepsin B thus preventing MEPE degradation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221291 | Phex (untagged) - Mouse phosphate regulating gene with homologies to endopeptidases on the X chromosome (hypophosphatemia, vitamin D resistant rickets) (Phex), (10ug) |
CNY 9,690.00 |
|
| MG227127 | Phex (tGFP-tagged) - Mouse phosphate regulating gene with homologies to endopeptidases on the X chromosome (hypophosphatemia vitamin D resistant rickets) (Phex), (10ug) |
CNY 6,370.00 |
|
| MR227127L3 | Lenti ORF clone of Phex (Myc-DDK-tagged) - Mouse phosphate regulating gene with homologies to endopeptidases on the X chromosome (hypophosphatemia, vitamin D resistant rickets) (Phex) |
CNY 7,700.00 |
|
| MR227127L4 | Lenti ORF clone of Phex (mGFP-tagged) - Mouse phosphate regulating gene with homologies to endopeptidases on the X chromosome (hypophosphatemia, vitamin D resistant rickets) (Phex) |
CNY 7,700.00 |
