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Retsat (NM_026159) Mouse Tagged Lenti ORF Clone
CAT＃: MR224429L3
- LentiORF®
Lenti ORF clone of Retsat (Myc-DDK-tagged) - Mouse retinol saturase (all trans retinol 13,14 reductase) (Retsat)
"NM_026159" in other vectors (4)
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CNY 7,220.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 0610039N19Rik; C80029; MMT-7; Ppsig |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR224429).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026159 |
| ORF Size | 1827 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026159.4 |
| RefSeq Size | 1942 bp |
| RefSeq ORF | 1830 bp |
| Locus ID | 67442 |
| UniProt ID | Q64FW2 |
| Gene Summary | Catalyzes the saturation of all-trans-retinol to all-trans-13,14-dihydroretinol (PubMed:15358783, PubMed:17253779, PubMed:19139408). Does not exhibit any activity toward all-trans-retinoic acid, nor 9-cis, 11-cis or 13-cis-retinol isomers (PubMed:15358783). May play a role in the metabolism of vitamin A (PubMed:15358783, PubMed:17253779). Independently of retinol conversion, may regulate liver metabolism upstream of MLXIPL/ChREBP (PubMed:28855500). Required for adipocyte differentiation in a 3T3-L1 cell culture model (PubMed:19139408). This effect seems not to mimic the in vivo situation in which animals show increased adiposity in the absence of RETSAT (PubMed:19940255).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219629 | Retsat (untagged) - Mouse retinol saturase (all trans retinol 13,14 reductase) (Retsat), (10ug) |
CNY 7,790.00 |
|
| MG224429 | Retsat (tGFP-tagged) - Mouse retinol saturase (all trans retinol 1314 reductase) (Retsat), (10ug) |
CNY 5,890.00 |
|
| MR224429 | Retsat (Myc-DDK-tagged) - Mouse retinol saturase (all trans retinol 13,14 reductase) (Retsat) |
CNY 4,536.00 |
|
| MR224429L4 | Lenti ORF clone of Retsat (mGFP-tagged) - Mouse retinol saturase (all trans retinol 13,14 reductase) (Retsat) |
CNY 7,220.00 |
