United States
Germany
Japan
United Kingdom
China
Retsat (NM_026159) Mouse Tagged ORF Clone
CAT＃: MG224429
- TrueORF®
Retsat (tGFP-tagged) - Mouse retinol saturase (all trans retinol 1314 reductase) (Retsat), (10ug)
ORF Plasmid: DDK
"NM_026159" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,890.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 0610039N19Rik; C80029; MMT-7; Ppsig |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224429 representing NM_026159
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGATCACTGCTCTGCTGCTGGCCGTGCTGCTGCTGGTGATCCTCCACAGGGTCTACGTGGGCCTTT ACGCTGCAAGTTCCCCGAACCCCTTCGCCGAGGATGTCAAGCGACCGCCTGAACCCCTGGTGACCGACAA GGAGGCTAGGAAGAAAGTTCTCAAACAAGCTTTCTCAGTCAGCCGAGTACCAGAGAAGCTGGATGCAGTG GTGATCGGCAGCGGCATTGGGGGACTGGCCTCAGCTGCGGTTCTAGCTAAAGCTGGCAAGAGAGTCCTTG TGCTGGAACAACATACCAAGGCGGGCGGCTGTTGTCATACCTTTGGGGAAAATGGCCTTGAATTTGACAC TGGAATTCATTATATTGGACGAATGCGGGAGGGCAACATTGGCCGTTTTATCTTGGACCAGATCACTGAA GGGCAACTGGACTGGGCCCCCATGGCCTCCCCTTTTGACTTGATGATACTAGAAGGGCCCAATGGCCGAA AGGAGTTCCCCATGTACAGTGGGAGGAAAGAATACATCCAGGGCCTTAAGAAGAAGTTCCCCAAGGAAGA AGCTGTCATTGACAAGTACATGGAGTTGGTTAAGGTGGTGGCCCGTGGAGTCTCTCATGCAGTTCTACTC AAGTTCCTCCCATTGCCCTTGACTCAGCTCCTCAGCAAGTTTGGGCTACTGACTCGTTTCTCTCCATTCT GCCGAGCGTCTACGCAGAGCCTAGCTGAAGTCCTGCAGCAGCTTGGGGCTTCCCGTGAGCTCCAGGCTGT TCTCAGCTACATCTTCCCCACTTACGGAGTAACTCCCAGCCACACCGCCTTTTCCTTGCATGCTCTGCTG GTTGACCACTACATACAAGGGGCATATTACCCTCGAGGGGGTTCCAGTGAGATCGCCTTCCATACCATCC CTTTGATTCAGCGGGCCGGGGGCGCTGTCCTCACCAGGGCCACTGTACAGAGTGTGCTGCTGGACTCAGC TGGGAGAGCGTGTGGTGTCAGTGTGAAGAAGGGACAAGAGCTGGTGAACATCTACTGCCCAGTTGTCATC TCCAATGCGGGAATGTTCAATACCTATCAGCACTTGTTGCCAGAGACTGTCCGCCATCTGCCAGATGTGA AGAAGCAGCTGGCGATGGTAAGGCCTGGTCTGAGCATGCTCTCAATCTTCATCTGTCTGAAAGGCACCAA GGAGGACCTGAAGCTTCAGTCCACCAACTACTATGTTTATTTTGACACAGACATGGACAAAGCGATGGAG CGCTATGTCTCTATGCCCAAGGAAAAGGCTCCAGAACACATTCCCCTTCTCTTCATTGCCTTCCCATCAA GCAAGGATCCAACCTGGGAGGAGCGATTCCCAGACCGATCCACAATGACTGCGCTGGTACCCATGGCCTT TGAATGGTTCGAGGAGTGGCAGGAGGAGCCAAAGGGCAAGCGTGGTGTTGACTATGAGACCCTCAAAAAT GCCTTCGTGGAAGCCTCTATGTCGGTGATCATGAAACTGTTCCCACAGCTGGAGGGCAAGGTGGAGAGTG TGACTGGAGGGTCACCACTGACCAACCAGTACTATCTGGCTGCACCCCGAGGAGCTACCTATGGAGCTGA CCATGACTTGGCTCGGCTGCATCCTCATGCAATGGCTTCCATAAGAGCCCAAACCCCCATCCCCAACCTC TACCTGACAGGCCAAGATATCTTCACCTGTGGGCTGATGGGGGCCCTGCAGGGGGCCTTGCTGTGCAGCA GTGCCATCCTGAAACGGAACTTGTACTCAGATCTGCAGGCTCTTGGCTCAAAGGTCAAGGCACAAAAGAA GAAGATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224429 representing NM_026159
Red=Cloning site Green=Tags(s) MWITALLLAVLLLVILHRVYVGLYAASSPNPFAEDVKRPPEPLVTDKEARKKVLKQAFSVSRVPEKLDAV VIGSGIGGLASAAVLAKAGKRVLVLEQHTKAGGCCHTFGENGLEFDTGIHYIGRMREGNIGRFILDQITE GQLDWAPMASPFDLMILEGPNGRKEFPMYSGRKEYIQGLKKKFPKEEAVIDKYMELVKVVARGVSHAVLL KFLPLPLTQLLSKFGLLTRFSPFCRASTQSLAEVLQQLGASRELQAVLSYIFPTYGVTPSHTAFSLHALL VDHYIQGAYYPRGGSSEIAFHTIPLIQRAGGAVLTRATVQSVLLDSAGRACGVSVKKGQELVNIYCPVVI SNAGMFNTYQHLLPETVRHLPDVKKQLAMVRPGLSMLSIFICLKGTKEDLKLQSTNYYVYFDTDMDKAME RYVSMPKEKAPEHIPLLFIAFPSSKDPTWEERFPDRSTMTALVPMAFEWFEEWQEEPKGKRGVDYETLKN AFVEASMSVIMKLFPQLEGKVESVTGGSPLTNQYYLAAPRGATYGADHDLARLHPHAMASIRAQTPIPNL YLTGQDIFTCGLMGALQGALLCSSAILKRNLYSDLQALGSKVKAQKKKM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026159 |
| ORF Size | 1827 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026159.5 |
| RefSeq Size | 1942 bp |
| RefSeq ORF | 1830 bp |
| Locus ID | 67442 |
| UniProt ID | Q64FW2 |
| Gene Summary | Catalyzes the saturation of all-trans-retinol to all-trans-13,14-dihydroretinol (PubMed:15358783, PubMed:17253779, PubMed:19139408). Does not exhibit any activity toward all-trans-retinoic acid, nor 9-cis, 11-cis or 13-cis-retinol isomers (PubMed:15358783). May play a role in the metabolism of vitamin A (PubMed:15358783, PubMed:17253779). Independently of retinol conversion, may regulate liver metabolism upstream of MLXIPL/ChREBP (PubMed:28855500). Required for adipocyte differentiation in a 3T3-L1 cell culture model (PubMed:19139408). This effect seems not to mimic the in vivo situation in which animals show increased adiposity in the absence of RETSAT (PubMed:19940255).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219629 | Retsat (untagged) - Mouse retinol saturase (all trans retinol 13,14 reductase) (Retsat), (10ug) |
CNY 7,790.00 |
|
| MR224429 | Retsat (Myc-DDK-tagged) - Mouse retinol saturase (all trans retinol 13,14 reductase) (Retsat) |
CNY 4,536.00 |
|
| MR224429L3 | Lenti ORF clone of Retsat (Myc-DDK-tagged) - Mouse retinol saturase (all trans retinol 13,14 reductase) (Retsat) |
CNY 7,220.00 |
|
| MR224429L4 | Lenti ORF clone of Retsat (mGFP-tagged) - Mouse retinol saturase (all trans retinol 13,14 reductase) (Retsat) |
CNY 7,220.00 |
