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Retsat (NM_026159) Mouse Untagged Clone
CAT＃: MC219629
Retsat (untagged) - Mouse retinol saturase (all trans retinol 13,14 reductase) (Retsat), (10ug)
CNY 7,790.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 0610039N19Rik; C80029; MMT-7; Ppsig |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC219629 representing NM_026159
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGATCACTGCTCTGCTGCTGGCCGTGCTGCTGCTGGTGATCCTCCACAGGGTCTACGTGGGCCTTT ACGCTGCAAGTTCCCCGAACCCCTTCGCCGAGGATGTCAAGCGACCGCCTGAACCCCTGGTGACCGACAA GGAGGCTAGGAAGAAAGTTCTCAAACAAGCTTTCTCAGTCAGCCGAGTACCAGAGAAGCTGGATGCAGTG GTGATCGGCAGCGGCATTGGGGGACTGGCCTCAGCTGCGGTTCTAGCTAAAGCTGGCAAGAGAGTCCTTG TGCTGGAACAACATACCAAGGCGGGCGGCTGTTGTCATACCTTTGGGGAAAATGGCCTTGAATTTGACAC TGGAATTCATTATATTGGACGAATGCGGGAGGGCAACATTGGCCGTTTTATCTTGGACCAGATCACTGAA GGGCAACTGGACTGGGCCCCCATGGCCTCCCCTTTTGACTTGATGATACTAGAAGGGCCCAATGGCCGAA AGGAGTTCCCCATGTACAGTGGGAGGAAAGAATACATCCAGGGCCTTAAGAAGAAGTTCCCCAAGGAAGA AGCTGTCATTGACAAGTACATGGAGTTGGTTAAGGTGGTGGCCCGTGGAGTCTCTCATGCAGTTCTACTC AAGTTCCTCCCATTGCCCTTGACTCAGCTCCTCAGCAAGTTTGGGCTACTGACTCGTTTCTCTCCATTCT GCCGAGCGTCTACGCAGAGCCTAGCTGAAGTCCTGCAGCAGCTTGGGGCTTCCCGTGAGCTCCAGGCTGT TCTCAGCTACATCTTCCCCACTTACGGAGTAACTCCCAGCCACACCGCCTTTTCCTTGCATGCTCTGCTG GTTGACCACTACATACAAGGGGCATATTACCCTCGAGGGGGTTCCAGTGAGATCGCCTTCCATACCATCC CTTTGATTCAGCGGGCCGGGGGCGCTGTCCTCACCAGGGCCACTGTACAGAGTGTGCTGCTGGACTCAGC TGGGAGAGCGTGTGGTGTCAGTGTGAAGAAGGGACAAGAGCTGGTGAACATCTACTGCCCAGTTGTCATC TCCAATGCGGGAATGTTCAATACCTATCAGCACTTGTTGCCAGAGACTGTCCGCCATCTGCCAGATGTGA AGAAGCAGCTGGCGATGGTAAGGCCTGGTCTGAGCATGCTCTCAATCTTCATCTGTCTGAAAGGCACCAA GGAGGACCTGAAGCTTCAGTCCACCAACTACTATGTTTATTTTGACACAGACATGGACAAAGCGATGGAG CGCTATGTCTCTATGCCCAAGGAAAAGGCTCCAGAACACATTCCCCTTCTCTTCATTGCCTTCCCATCAA GCAAGGATCCAACCTGGGAGGAGCGATTCCCAGACCGATCCACAATGACTGCGCTGGTACCCATGGCCTT TGAATGGTTCGAGGAGTGGCAGGAGGAGCCAAAGGGCAAGCGTGGTGTTGACTATGAGACCCTCAAAAAT GCCTTCGTGGAAGCCTCTATGTCGGTGATCATGAAACTGTTCCCACAGCTGGAGGGCAAGGTGGAGAGTG TGACTGGAGGGTCACCACTGACCAACCAGTACTATCTGGCTGCACCCCGAGGAGCTACCTATGGAGCTGA CCATGACTTGGCTCGGCTGCATCCTCATGCAATGGCTTCCATAAGAGCCCAAACCCCCATCCCCAACCTC TACCTGACAGGCCAAGATATCTTCACCTGTGGGCTGATGGGGGCCCTGCAGGGGGCCTTGCTGTGCAGCA GTGCCATCCTGAAACGGAACTTGTACTCAGATCTGCAGGCTCTTGGCTCAAAGGTCAAGGCACAAAAGAA GAAGATGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_026159 |
| Insert Size | 1830 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_026159.4, NP_080435.3 |
| RefSeq Size | 1942 bp |
| RefSeq ORF | 1830 bp |
| Locus ID | 67442 |
| UniProt ID | Q64FW2 |
| Gene Summary | Catalyzes the saturation of all-trans-retinol to all-trans-13,14-dihydroretinol (PubMed:15358783, PubMed:17253779, PubMed:19139408). Does not exhibit any activity toward all-trans-retinoic acid, nor 9-cis, 11-cis or 13-cis-retinol isomers (PubMed:15358783). May play a role in the metabolism of vitamin A (PubMed:15358783, PubMed:17253779). Independently of retinol conversion, may regulate liver metabolism upstream of MLXIPL/ChREBP (PubMed:28855500). Required for adipocyte differentiation in a 3T3-L1 cell culture model (PubMed:19139408). This effect seems not to mimic the in vivo situation in which animals show increased adiposity in the absence of RETSAT (PubMed:19940255).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG224429 | Retsat (tGFP-tagged) - Mouse retinol saturase (all trans retinol 1314 reductase) (Retsat), (10ug) |
CNY 5,890.00 |
|
| MR224429 | Retsat (Myc-DDK-tagged) - Mouse retinol saturase (all trans retinol 13,14 reductase) (Retsat) |
CNY 4,536.00 |
|
| MR224429L3 | Lenti ORF clone of Retsat (Myc-DDK-tagged) - Mouse retinol saturase (all trans retinol 13,14 reductase) (Retsat) |
CNY 7,220.00 |
|
| MR224429L4 | Lenti ORF clone of Retsat (mGFP-tagged) - Mouse retinol saturase (all trans retinol 13,14 reductase) (Retsat) |
CNY 7,220.00 |
