Ehmt2 (NM_145830) Mouse Tagged Lenti ORF Clone
CAT#: MR222714L3
- LentiORF®
Lenti ORF clone of Ehmt2 (Myc-DDK-tagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2), transcript variant long
"NM_145830" in other vectors (4)
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CNY 14,540.00
CNY 600.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Bat8; D17Ertd710e; G9a; KMT1C; NG36 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR222714).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_145830 |
ORF Size | 3792 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_145830.1 |
RefSeq Size | 4070 bp |
RefSeq ORF | 3792 bp |
Locus ID | 110147 |
UniProt ID | Q9Z148 |
Gene Summary | Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC224113 | Ehmt2 (untagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2), transcript variant long, (10ug) |
CNY 16,340.00 |
|
MG222714 | Ehmt2 (tGFP-tagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2) transcript variant long, (10ug) |
CNY 13,870.00 |
|
MR222714 | Ehmt2 (Myc-DDK-tagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2), transcript variant long |
CNY 13,200.00 |
|
MR222714L4 | Lenti ORF clone of Ehmt2 (mGFP-tagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2), transcript variant long |
CNY 14,540.00 |