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Ehmt2 (NM_145830) Mouse Tagged ORF Clone
CAT＃: MR222714
- TrueORF®
Ehmt2 (Myc-DDK-tagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2), transcript variant long
ORF Plasmid: tGFP
"NM_145830" in other vectors (4)
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CNY 13,200.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Bat8; D17Ertd710e; G9a; KMT1C; NG36 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222714 representing NM_145830
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGGTCTGCCGAGAGGGAGGGGGCTGATGCGGGCCCGGGGGCGGGGGCGTGCGGCCCCCACGGGCG GCCGCGGCCGCGGTCGGGGGGGCGCCCACCGAGGGCGAGGTAGGCCCCGAAGCCTGCTCTCGCTGCCCAG GGCCCAGGCGTCTTGGGCCCCCCAGCTGCCTGCCGGGCTGACCGGCCCCCCGGTTCCTTGTCTCCCCTCC CAGGGGGAGGCCCCCGCTGAGATGGGGGCGCTGCTGCTGGAGAAGGAGCCCCGAGGAGCCGCCGAGAGAG TTCATAGCTCTTTGGGGGACACCCCTCAGAGTGAGGAGACCCTTCCCAAGGCCAACCCCGACTCCTTGGA GCCTGCCGGCCCCTCCTCTCCGGCCTCTGTCACTGTCACCGTCGGCGATGAGGGGGCTGACACCCCTGTC GGGGCCGCATCACTCATCGGGGACGAACCCGAGAGCCTGGAGGGAGATGGGGGTCGCATCGTGCTGGGCC ATGCCACAAAGTCGTTCCCCTCTTCCCCCAGCAAGGGGGGTGCCTGTCCCAGTCGGGCCAAAATGTCAAT GACAGGGGCAGGAAAGTCGCCCCCCTCGGTCCAGAGTTTGGCCATGAGGCTGTTGAGCATGCCCGGGGCC CAGGGAGCTGCAACTGCTGGGCCTGAACCCTCTCCGGCAACAACTGCCGCCCAGGAGGGGCAGCCCAAAG TGCACCGAGCCCGGAAAACCATGTCCAAACCTAGCAACGGACAGCCTCCAATCCCTGAGAAGCGGCCCCC TGAAGTCCAGCATTTCCGCATGAGTGATGACATGCATCTGGGGAAGGTGACTTCAGATGTGGCCAAAAGG AGGAAGCTGAACTCTGGTAGCCTGTCCGAGGACTTGGGCTCTGCCGGGGGCTCAGGAGATATAATCCTGG AGAAGGGAGAGCCCAGGCCCCTGGAGGAGTGGGAGACGGTGGTGGGCGATGACTTCAGCCTGTACTATGA TGCGTACTCTGTGGATGAGCGGGTGGACTCTGACAGCAAGTCTGAAGTCGAAGCTCTAGCTGAACAGTTG AGTGAGGAGGAGGAGGAGGAAGAGGAGGAAGAAGAAGAAGAGGAGGAGGAGGAGGAAGAGGAGGAGGAGG AAGAAGAGGACGAGGAGTCGGGCAATCAGTCAGACAGGAGCGGTTCTAGTGGCCGGCGCAAGGCCAAGAA GAAATGGCGGAAAGACAGCCCGTGGGTGAAGCCATCTAGAAAACGGCGGAAACGAGAGCCTCCGAGGGCC AAGGAGCCAAGAGGAGTGAATGGTGTGGGTTCCTCAGGGCCCAGTGAGTACATGGAGGTTCCTCTGGGGT CCCTGGAGCTGCCCAGCGAGGGGACCCTCTCCCCCAACCACGCTGGGGTCTCCAATGACACGTCTTCACT GGAGACAGAACGCGGGTTTGAGGAGCTGCCCCTCTGCAGCTGCCGCATGGAGGCTCCCAAGATTGACCGC ATCAGCGAGAGAGCAGGGCACAAGTGCATGGCCACAGAGAGTGTGGATGGAGAGCTCCTGGGCTGCAATG CTGCCATCCTTAAGCGGGAGACCATGCGGCCGTCTAGCCGCGTGGCGCTGATGGTGCTCTGTGAGGCCCA TCGAGCCCGCATGGTCAAGCACCATTGCTGCCCGGGCTGCGGCTACTTCTGCACAGCGGGCACCTTCCTG GAATGCCACCCCGACTTTCGTGTAGCTCACCGCTTCCATAAGGCCTGCGTATCCCAGCTCAATGGGATGG TCTTCTGTCCCCACTGTGGAGAGGATGCCTCAGAGGCCCAGGAGGTGACCATTCCTCGGGGCGATGGGGG AACACCCCCAATTGGCACCGCAGCTCCTGCTCTGCCACCCCTGGCACATGATGCCCCAGGGCGAGCGGAT ACCTCCCAGCCTAGCGCCCGAATGCGAGGGCATGGAGAGCCGCGGCGCCCGCCCTGTGATCCCCTGGCTG ACACCATCGACAGCTCAGGGCCTTCACTGACTCTGCCTAATGGGGGCTGCCTCTCCGCTGTGGGTCTGCC CCCAGGGCCGGGCAGGGAAGCCCTGGAAAAAGCCTTGGTCATCCAGGAGTCTGAGAGGCGGAAGAAGCTG CGATTCCACCCACGGCAGCTGTACCTGTCGGTGAAGCAGGGGGAGCTGCAGAAGGTGATCCTTATGCTGT TAGACAACCTGGACCCCAACTTCCAGAGCGACCAGCAGAGCAAGCGCACGCCCCTGCACGCGGCCGCCCA GAAGGGGTCGGTAGAGATCTGTCATGTGCTGCTGCAGGCAGGAGCCAACATCAATGCCGTAGATAAGCAA CAACGCACGCCACTAATGGAGGCCGTGGTGAACAACCACCTGGAGGTGGCACGCTACATGGTGCAGTTAG GTGGCTGTGTCTACAGCAAGGAAGAGGATGGCTCCACCTGTCTACATCATGCAGCCAAAATTGGGAACTT GGAAATGGTCAGCCTGCTACTGAGCACAGGACAGGTGGACGTCAATGCCCAGGACAGTGGGGGCTGGACG CCCATCATCTGGGCAGCCGAGCACAAGCACATCGATGTGATTCGTATGCTGCTGACCCGGGGTGCCGATG TCACCCTGACTGACAATGAGGAAAACATCTGCCTGCACTGGGCCTCCTTCACGGGTAGTGCCGCCATCGC TGAGGTCCTTCTGAATGCCCAGTGTGATCTCCATGCTGTCAACTACCATGGGGACACGCCCCTGCACATA GCCGCCAGGGAGAGCTACCATGACTGTGTTCTGTTGTTCCTGTCTCGTGGAGCCAACCCTGAGCTTCGGA ACAAAGAAGGAGACACGGCATGGGATCTGACCCCAGAGCGCTCTGATGTGTGGTTTGCACTGCAGCTCAA TCGAAAGCTTAGGCTTGGGGTAGGGAACCGGGCTGTCCGCACCGAGAAGATCATCTGCCGGGACGTAGCC CGAGGCTATGAGAATGTACCCATCCCCTGTGTCAATGGTGTGGATGGGGAGCCGTGCCCGGAGGACTACA AGTACATCTCTGAGAACTGCGAGACATCGACCATGAACATCGACCGCAACATCACCCATCTGCAGCACTG CACGTGTGTGGATGACTGCTCCAGCTCCAATTGCCTATGTGGTCAGCTCAGTATCCGATGCTGGTATGAC AAGGACGGGCGGCTGCTCCAGGAGTTTAACAAGATCGAGCCCCCCCTGATCTTTGAGTGTAACCAGGCAT GCTCCTGCTGGAGAAGCTGCAAGAACCGCGTGGTGCAGAGCGGCATCAAGGTACGGCTGCAGCTCTACCG GACTGCCAAGATGGGCTGGGGGGTCCGAGCCTTGCAGACCATCCCCCAGGGCACGTTCATCTGCGAGTAT GTAGGAGAGCTGATCTCTGATGCCGAGGCTGATGTGAGAGAGGATGATTCTTACCTCTTCGATTTAGATA ACAAGGATGGCGAGGTTTACTGCATTGATGCCCGTTACTATGGCAACATCAGCCGATTCATTAACCACCT GTGTGACCCCAACATCATCCCTGTCCGGGTTTTCATGCTGCACCAAGATCTACGGTTCCCACGCATTGCC TTCTTCAGCTCCAGGGACATCCGGACTGGGGAGGAGCTGGGCTTTGACTACGGTGACCGATTCTGGGACA TCAAGAGCAAGTATTTCACCTGCCAGTGTGGCTCTGAGAAGTGCAAGCATTCAGCGGAGGCCATCGCCCT GGAGCAGAGCCGCCTGGCCCGGCTGGACCCCCACCCGGAGCTGCTCCCTGACCTCAGCTCCCTGCCCCCC ATCAACACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222714 representing NM_145830
Red=Cloning site Green=Tags(s) MRGLPRGRGLMRARGRGRAAPTGGRGRGRGGAHRGRGRPRSLLSLPRAQASWAPQLPAGLTGPPVPCLPS QGEAPAEMGALLLEKEPRGAAERVHSSLGDTPQSEETLPKANPDSLEPAGPSSPASVTVTVGDEGADTPV GAASLIGDEPESLEGDGGRIVLGHATKSFPSSPSKGGACPSRAKMSMTGAGKSPPSVQSLAMRLLSMPGA QGAATAGPEPSPATTAAQEGQPKVHRARKTMSKPSNGQPPIPEKRPPEVQHFRMSDDMHLGKVTSDVAKR RKLNSGSLSEDLGSAGGSGDIILEKGEPRPLEEWETVVGDDFSLYYDAYSVDERVDSDSKSEVEALAEQL SEEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQSDRSGSSGRRKAKKKWRKDSPWVKPSRKRRKREPPRA KEPRGVNGVGSSGPSEYMEVPLGSLELPSEGTLSPNHAGVSNDTSSLETERGFEELPLCSCRMEAPKIDR ISERAGHKCMATESVDGELLGCNAAILKRETMRPSSRVALMVLCEAHRARMVKHHCCPGCGYFCTAGTFL ECHPDFRVAHRFHKACVSQLNGMVFCPHCGEDASEAQEVTIPRGDGGTPPIGTAAPALPPLAHDAPGRAD TSQPSARMRGHGEPRRPPCDPLADTIDSSGPSLTLPNGGCLSAVGLPPGPGREALEKALVIQESERRKKL RFHPRQLYLSVKQGELQKVILMLLDNLDPNFQSDQQSKRTPLHAAAQKGSVEICHVLLQAGANINAVDKQ QRTPLMEAVVNNHLEVARYMVQLGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVNAQDSGGWT PIIWAAEHKHIDVIRMLLTRGADVTLTDNEENICLHWASFTGSAAIAEVLLNAQCDLHAVNYHGDTPLHI AARESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAVRTEKIICRDVA RGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYD KDGRLLQEFNKIEPPLIFECNQACSCWRSCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEY VGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIA FFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPDLSSLPP INT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145830 |
| ORF Size | 3792 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145830.3 |
| RefSeq Size | 4070 bp |
| RefSeq ORF | 3792 bp |
| Locus ID | 110147 |
| UniProt ID | Q9Z148 |
| MW | 138 kDa |
| Gene Summary | Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224113 | Ehmt2 (untagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2), transcript variant long, (10ug) |
CNY 16,340.00 |
|
| MG222714 | Ehmt2 (tGFP-tagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2) transcript variant long, (10ug) |
CNY 13,870.00 |
|
| MR222714L3 | Lenti ORF clone of Ehmt2 (Myc-DDK-tagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2), transcript variant long |
CNY 14,540.00 |
|
| MR222714L4 | Lenti ORF clone of Ehmt2 (mGFP-tagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2), transcript variant long |
CNY 14,540.00 |
