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Pkn1 (NM_177262) Mouse Tagged Lenti ORF Clone
CAT＃: MR211277L4
- LentiORF®
Lenti ORF clone of Pkn1 (mGFP-tagged) - Mouse protein kinase N1 (Pkn1), transcript variant 2
"NM_177262" in other vectors (4)
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CNY 10,260.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | DBK; F730027O18Rik; PAK1; Pkn; PRK1; Prkcl1; Stk3 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR211277).
|
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NM_177262 |
| ORF Size | 2838 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177262.4, NP_796236.2 |
| RefSeq Size | 3436 bp |
| RefSeq ORF | 2841 bp |
| Locus ID | 320795 |
| UniProt ID | P70268 |
| Gene Summary | PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203356 | Pkn1 (untagged) - Mouse protein kinase N1 (Pkn1), transcript variant 2, (10ug) |
CNY 6,936.00 |
|
| MG211277 | Pkn1 (tGFP-tagged) - Mouse protein kinase N1 (Pkn1) |
CNY 9,220.00 |
|
| MR211277 | Pkn1 (Myc-DDK-tagged) - Mouse protein kinase N1 (Pkn1), transcript variant 2 |
CNY 6,928.00 |
|
| MR211277L3 | Lenti ORF clone of Pkn1 (Myc-DDK-tagged) - Mouse protein kinase N1 (Pkn1), transcript variant 2 |
CNY 10,260.00 |
