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Pkn1 (NM_177262) Mouse Untagged Clone
CAT＃: MC203356
Pkn1 (untagged) - Mouse protein kinase N1 (Pkn1), transcript variant 2, (10ug)
CNY 6,936.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | DBK; F730027O18Rik; PAK1; Pkn; PRK1; Prkcl1; Stk3 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC052923
GCCAGGCCGGGCCGAGCGCAGCAGGCCGGAGCGCGCCGCGCGCCTGGGAGGGTCTGGGCGCCGCGCCTCC CGCCGCACCCGCCCGCGCGCTCGCAAGCCCTTCCTTGGCGCTGGGACCCCTGGCGGGCGGCCGGAGGACA TGGCCGGCGACGCCGTACAGAGTGAGCCTCGAAGCTGGTCGCTGCTGGAACAGCTGGGTCTGGCTGGGGC TGACCTGGCAGCCCCCGGGGTGCAGCAACAGCTGGAGTTAGAGCGAGAGCGACTGAAGCGGGAGATACGG AAGGAGCTGAAGCTGAAGGAGGGCGCTGAGAACCTGAGGCGAGCCACCACTGACCTGGGCCGTAGCCTGG CCCCTGTGGAGCTGCTGCTGCGGGGCTCCGCTCGACGCCTAGACTTACTGCACCAGCAGCTGCAGGAGCT GCATGCACATGTGGTGCTGCCTGACCCTGCAGCTGGAAGCGATGCTACCCAATCCCTTGCAGAGGGCAGC CCTATCTGCTCATCCACCAACCTGAGCAGAGTGGCTGGGCTGGAGAAGCAGCTGGCCATTGAGCTCAAGG TCAAACAGGGGGCAGAGAACATGATCCAGACCTATAGCAATGGCAGCAGCAAGGACCGGAAGCTGTTGTT GACAGCCCAGCAGATGCTGCAGGACAGTAAGACCAAGATTGACATCATCCGCATGCAGCTTCGAAGAGCG CTCCAGGCACTACAGGCTGGAGAGCTGGAGAGTCAGGCAGCTCCTGATGAAGCCCAAGGAGATCCAGAAC TGGGAGCCGTAGAGCTACGCATTGAGGAGCTACGACACCATTTTCGAGTGGAGCATGCAGTGGCAGAAGG TGCCAAGAATGTCCTGCGCCTGCTCAGTGGGGCAAAGGCCCCAGACCGCAAGGCAGTCAGCGAGGCTCAG GAGAAATTGACTGAGTCCAACCAGAAGCTGGGCTTGCTGCGGGAATCACTGGAGAGGCGCCTTGGGGAGT TGCCTGCCGATCACCCCAAGGGGCGGCTGCTTCGGGAGGAGCTCACTGCAGCCTCGTCCTCAGCCTTCAG CGCCATACTGCCTGGGCCCTTCCCTGCCACTCACTACAGCACCTTGAGCAAGCCTGCACCGCTCACAGGG ACCCTGGAAGTACGAGTGGTGGGCTGCAAAAACCTTCCCGAGACCATCCCCTGGAGCCCTCCCCCCTCAG TGGGGGCATCTGGGACCCCCGAAAGCCGCACTCCGTTCCTGAGTCGCCCCGCTCGGGGCCTTTACAGCCG GAGTGGAAGCCTTAGTGGACGGAGCAGCCTCAGAGGGGAGGCAGAGAATGCCACTGAGGTCAGCACCGTG CTCAAGCTGGACAACACAGTGGTGGGGCAAACAGCCTGGAAGCCATGCGGCCCCAATGCCTGGGACCAGA GCTTTACCCTGGAGCTGGAGAGGGCTCGGGAGCTGGAGCTGGCTGTGTTCTGGCGAGACCAGAGGGGTCT GTGTGCTCTCAAATTTCTGAAGTTGGAGGACTTCTTGGACAACGAGAGGCATGAGGTGCAGCTGGACATG GAACCCCAGGGCTGCCTGGTGGCTGAGGTCACTTTCCGAAACCCCATCATCGAGCGGATCCCTAGGCTCC AAAGGCAGAAAAAAATCTTCTCCAAGCAGCAAGGGAAGGCATTTCAGCGAGCCAGACAGATGAACATAGA TGTGGCGACTTGGGTGCGGCTGCTCCGTAGACTCATCCCTAGTGCTGTGGCCACTGGCACCTTCAGTCCC AATGCATCTCCAGGTGCTGAGATCCGGCACACTGGAGACATATCCATGGAGAAATTGAATCTCGGTGCTG ACTCAGACAGCTCGTCCCAAAAGAGCCCACCAGGGCTGCCCTCCACCTCATGCAGCCTGAGTTCTCCAAC CCATGAATCCACCACATCTCCAGAGCTGCCTTCAGAGACCCAGGAGACTCCAGGCCCTGGCCTGTGCAGC CCCTTGAGAAAGTCGCCCCTGACACTTGAGGACTTCAAGTTCCTGGCCGTGCTTGGCCGGGGTCACTTTG GAAAGGTGCTGCTGTCTGAATTCCGCTCCAGTGGGGAGCTCTTTGCCATCAAAGCCTTGAAGAAAGGTGA CATTGTAGCCCGAGATGAGGTTGAGAGCCTGATGTGTGAGAAGCGGATTTTGGCGGCCGTGACCAGGGCA GGACATCCCTTCCTGGTGAACCTTTTCGGCTGTTTCCAGACCCCAGAGCACGTGTGCTTTGTGATGGAGT ACTCGGCGGGTGGAGACCTGATGCTGCACATTCATAGCGACGTGTTCTCAGAGCCTCGGGCTGTCTTCTA TTCGGCCTGTGTGGTGCTGGGACTGCAGTTCCTCCATGAACACAAGATTGTCTACAGGGACCTGAAGTTG GACAATTTGCTCCTGGATACTGAGGGCTACGTCAAGATCGCAGACTTTGGCCTCTGCAAGGAGGGGATGG GCTATGGGGACCGGACCAGCACGTTCTGCGGAACTCCGGAGTTCCTGGCGCCGGAAGTGCTCACAGACAC ATCCTACACGCGAGCAGTGGACTGGTGGGGACTGGGCGTGCTGCTCTATGAGATGTTGGTTGGAGAGTCT CCGTTCCCTGGGGATGATGAGGAGGAGGTATTTGACAGCATTGTCAACGACGAAGTTCGCTATCCCCGCT TCCTGTCTGCAGAGGCCATCGGCATCATGAGAAGGCTACTGCGGAGGAACCCGGAGCGGAGGCTGGGGTC CACTGAGCGCGATGCAGAAGATGTGAAAAAACAGCCTTTCTTCCGGTCTCTGGGCTGGGATGTCCTGCTG GCCCGCCGCTTGCCTCCACCCTTCGTGCCTACACTTTCAGGGCGCACAGATGTCAGCAACTTCGATGAGG AGTTCACTGGGGAGGCCCCCACACTGAGTCCTCCCCGGGATGCACGGCCCCTCACAGCTGCGGAGCAGGC AGCCTTCCGGGATTTCGACTTTGTGGCCGGAGGCTACTAGCCCCAAGCCCCTGCCTTACCCAAGAGTTCT TGATTTTTTAAAAAACAAGCCTTTGGGGTTTACTCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_177262 |
| Insert Size | 2841 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC052923, AAH52923 |
| RefSeq Size | 3124 bp |
| RefSeq ORF | 2841 bp |
| Locus ID | 320795 |
| UniProt ID | P70268 |
| Gene Summary | PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains a different segment for its 5' UTR and 5' coding region, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct N-terminus when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211277 | Pkn1 (tGFP-tagged) - Mouse protein kinase N1 (Pkn1) |
CNY 9,220.00 |
|
| MR211277 | Pkn1 (Myc-DDK-tagged) - Mouse protein kinase N1 (Pkn1), transcript variant 2 |
CNY 6,928.00 |
|
| MR211277L3 | Lenti ORF clone of Pkn1 (Myc-DDK-tagged) - Mouse protein kinase N1 (Pkn1), transcript variant 2 |
CNY 10,260.00 |
|
| MR211277L4 | Lenti ORF clone of Pkn1 (mGFP-tagged) - Mouse protein kinase N1 (Pkn1), transcript variant 2 |
CNY 10,260.00 |
