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Mcm8 (BC046780) Mouse Tagged ORF Clone
CAT＃: MR210873
- TrueORF®
Mcm8 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 8 (S
ORF Plasmid: tGFP
"BC046780" in other vectors (4)
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CNY 6,104.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5730432L01Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210873 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGTGCTTATAGAGGCAGAGGTTTTGGACGAGGAAGATTCCAAAGCTGGAAAAGAGGAAGAGGTG GTGGGAACTTCTCAGGAAGGTGGAGAGAAAGAGAAAACAGAGTTGACCTGAATGAAGCTTCAGGAAAGCA CGCTTCTGCACAAGCCTCACAACCGTTACTTCAACAGTCAACATTGGATCAGTTTATCCCGTATAAGGGC TGGAAGCTTTACTTCTCTGAAGTTTACAGTAATAACTCTCCCTTTATTGAGAAGATTCAAGCATTTGAGA AATTTTTCACAAGGCATATCGACTTATACGATAAGGATGAAATAGAAAGAAAAGGAAGCATTTTGGTGGA TTTTAAAGAACTGACAAAAGCTGATGAAATAACTAACTTGATACCCGATATAGAAAACGCACTAAGAGAT GCCCCTGAGAAAACACTGGCGTGTATGGGGCTGGCAATACATCAGGTATTAACTAAGGACCTTGAAAGGC ATGCCGCCGAATTACAAGCTCAAGAGGGATTGTCTAACGGTGGAGAGACAATGGTAAATGTACCACATAT TTATGCAAGAGTGTACAACTATGAGCCCTTGACACACCTCAAGAATATCCGAGCAACTTGCTATGGGAAA TACATCTCTATACGAGGGACTGTGGTCCGGGTCAGCAACATAAAGCCTCTTTGCACCAACATGGCTTTTC AGTGCGCTGCATGTGGAGAGATTCAGAGCTTTCCTCTGCCAGATGGAAAATACACCCTTCCTACAAAGTG TCCTGTGCCTGCGTGCCGAGGGAGGTCATTTGCTCCACTGCGCAGCTCTCCTCTCACAGTTACACTGGAC TGGCAGTTGATCAAAATCCAGGAGCTGATGTCTGATGCACAGAGAGAAGCTGGTCGGATCCCTCGGACGA TAGAATGTGAACTTGTTCACGATCTTGTAGATAGTTGTGTCCCAGGAGATACAGTGACTGTTACTGGAAT TGTCAAAGTCTCCAATTCTGAAGAAGGTTCTCGAAATAAGAATGATAAGTGCATGTTCCTTTTGTACATT GAGGCAAATTCTGTTAGCAACAGCAAGGGGCCGAAAGCACAGACTGCAGAAGATGGTTGCAAGCATGGGA CACTGATGGAGTTCTCCCTTAAAGACCTCTATGCCATCCGAGAGATCCAGGCTGAAGAGAACCTGCTCAA GCTCGTTGTCAACTCACTCTGTCCTGTCATTTTTGGTCATGAACTCGTCAAAGCAGGTCTGACGTTAGCA CTCTTTGGCGGTAGCCAGAAGTACGCAGATGACAAAAACAGAATTCCCATTCGAGGAGACCCACATGTCC TGATTGTTGGAGATCCAGGCTTGGGGAAGAGTCAGATGCTACAGGCAGCATGCAACGTGGCGCCACGTGG TGTGTATGTTTGTGGAAATACCACCACCAGCTCTGGTCTCACTGTGACTCTTTCAAAGGACAGTTCCTCT GGAGATTTTGCTTTGGAAGCTGGTGCCCTTGTACTTGGTGACCAAGGCATCTGTGGAATAGATGAATTTG ATAAAATGGGGAACCAACATCAAGCCCTGTTAGAAGCCATGGAACAGCAGAGTATTAGCCTTGCGAAGGC TGGGGTAGTTTGCAGCCTCCCTGCAAGAACTTCCATTGTTGCTGCTGCAAATCCAGTCGGAGGACACTAC AATAAAGCCAGAACGGTTTCTGAGAATTTAAAGATGGGGAGTGCCCTACTGTCCAGATTCGATTTGGTCT TTATCTTGTTAGATACGCCAAATGAACAGCATGACCACTTACTTTCTGAACATGTGATCGCGATAAGAGC TGGGAAGCAGAAAGCGGTTAGCAGTGCCACAGTCACTCGTGTGCTCAGTCAAGACTCAAATACTTCTGTA CTTGAAGTGGTTTCTGAGAAACCATTATCAGAAAGACTAAAGGTGGCTCCTGGAGAACAGACAGATCCGA TTCCACATCAGCTGTTGAGGAAGTACATTGGCTATGCACGGCAGTATGTCCACCCAAGGTTATCTACGGA CGCTGCTCAGGCCCTTCAGGATTTCTACCTGGAGCTCCGCAAGCAGAGCCAGCGCGTGGGCAGCTCACCC ATCACCACTCGGCAGCTGGAGTCTTTGATCCGTCTGACAGAGGCACGGGCAAGGTTAGAATTGAGAGAGG AAGCAACTAGAGAAGATGCTGAAGATATAATTGAAATTATGAAGCATAGCATGCTAGGAACTTACTCAGA TGAATTCGGAAACCTGGACTTTGAGCGATCCCAGCATGGCTCTGGGATGAGCAACAGGTCAACAGCAAAA AGATTTATTTCTGCTCTCAACAGCATTGCTGAAAGAACTTATAACAACATATTTCAATATCATCAACTTC GTCAGATTGCTAAAGAACTAAACATTCAGGTTGCCGATTTTGAAAACTTCATTGGATCACTGAATGACCA AGGTTATCTCTTGAAGAAAGGCCCAAAGATTTACCAACTTCAAACTATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210873 protein sequence
Red=Cloning site Green=Tags(s) MSGAYRGRGFGRGRFQSWKRGRGGGNFSGRWRERENRVDLNEASGKHASAQASQPLLQQSTLDQFIPYKG WKLYFSEVYSNNSPFIEKIQAFEKFFTRHIDLYDKDEIERKGSILVDFKELTKADEITNLIPDIENALRD APEKTLACMGLAIHQVLTKDLERHAAELQAQEGLSNGGETMVNVPHIYARVYNYEPLTHLKNIRATCYGK YISIRGTVVRVSNIKPLCTNMAFQCAACGEIQSFPLPDGKYTLPTKCPVPACRGRSFAPLRSSPLTVTLD WQLIKIQELMSDAQREAGRIPRTIECELVHDLVDSCVPGDTVTVTGIVKVSNSEEGSRNKNDKCMFLLYI EANSVSNSKGPKAQTAEDGCKHGTLMEFSLKDLYAIREIQAEENLLKLVVNSLCPVIFGHELVKAGLTLA LFGGSQKYADDKNRIPIRGDPHVLIVGDPGLGKSQMLQAACNVAPRGVYVCGNTTTSSGLTVTLSKDSSS GDFALEAGALVLGDQGICGIDEFDKMGNQHQALLEAMEQQSISLAKAGVVCSLPARTSIVAAANPVGGHY NKARTVSENLKMGSALLSRFDLVFILLDTPNEQHDHLLSEHVIAIRAGKQKAVSSATVTRVLSQDSNTSV LEVVSEKPLSERLKVAPGEQTDPIPHQLLRKYIGYARQYVHPRLSTDAAQALQDFYLELRKQSQRVGSSP ITTRQLESLIRLTEARARLELREEATREDAEDIIEIMKHSMLGTYSDEFGNLDFERSQHGSGMSNRSTAK RFISALNSIAERTYNNIFQYHQLRQIAKELNIQVADFENFIGSLNDQGYLLKKGPKIYQLQTM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC046780 |
| ORF Size | 2499 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC046780, AAH46780 |
| RefSeq Size | 2908 bp |
| RefSeq ORF | 2501 bp |
| Locus ID | 66634 |
| MW | 92.4 kDa |
| Gene Summary | Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity. Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs. The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression. However, may play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC) (By similarity). Probably by regulating HR, plays a key role during gametogenesis (PubMed:22771120). Stabilizes MCM9 protein (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220811 | Mcm8 (untagged) - Mouse minichromosome maintenance deficient 8 (S, (10ug) |
CNY 9,120.00 |
|
| MG210873 | Mcm8 (tGFP-tagged) - Mouse minichromosome maintenance deficient 8 (S |
CNY 5,230.00 |
|
| MR210873L3 | Lenti ORF clone of Mcm8 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 8 (S |
CNY 6,650.00 |
|
| MR210873L4 | Lenti ORF clone of Mcm8 (mGFP-tagged) - Mouse minichromosome maintenance deficient 8 (S |
CNY 6,650.00 |
