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Mcm8 (BC046780) Mouse Untagged Clone
CAT＃: MC220811
Mcm8 (untagged) - Mouse minichromosome maintenance deficient 8 (S, (10ug)
CNY 9,120.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 5730432L01Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC046780
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGTGCTTATAGAGGCAGAGGTTTTGGACGAGGAAGATTCCAAAGCTGGAAAAGAGGAAGAGGTG GTGGGAACTTCTCAGGAAGGTGGAGAGAAAGAGAAAACAGAGTTGACCTGAATGAAGCTTCAGGAAAGCA CGCTTCTGCACAAGCCTCACAACCGTTACTTCAACAGTCAACATTGGATCAGTTTATCCCGTATAAGGGC TGGAAGCTTTACTTCTCTGAAGTTTACAGTAATAACTCTCCCTTTATTGAGAAGATTCAAGCATTTGAGA AATTTTTCACAAGGCATATCGACTTATACGATAAGGATGAAATAGAAAGAAAAGGAAGCATTTTGGTGGA TTTTAAAGAACTGACAAAAGCTGATGAAATAACTAACTTGATACCCGATATAGAAAACGCACTAAGAGAT GCCCCTGAGAAAACACTGGCGTGTATGGGGCTGGCAATACATCAGGTATTAACTAAGGACCTTGAAAGGC ATGCCGCCGAATTACAAGCTCAAGAGGGATTGTCTAACGGTGGAGAGACAATGGTAAATGTACCACATAT TTATGCAAGAGTGTACAACTATGAGCCCTTGACACACCTCAAGAATATCCGAGCAACTTGCTATGGGAAA TACATCTCTATACGAGGGACTGTGGTCCGGGTCAGCAACATAAAGCCTCTTTGCACCAACATGGCTTTTC AGTGCGCTGCATGTGGAGAGATTCAGAGCTTTCCTCTGCCAGATGGAAAATACACCCTTCCTACAAAGTG TCCTGTGCCTGCGTGCCGAGGGAGGTCATTTGCTCCACTGCGCAGCTCTCCTCTCACAGTTACACTGGAC TGGCAGTTGATCAAAATCCAGGAGCTGATGTCTGATGCACAGAGAGAAGCTGGTCGGATCCCTCGGACGA TAGAATGTGAACTTGTTCACGATCTTGTAGATAGTTGTGTCCCAGGAGATACAGTGACTGTTACTGGAAT TGTCAAAGTCTCCAATTCTGAAGAAGGTTCTCGAAATAAGAATGATAAGTGCATGTTCCTTTTGTACATT GAGGCAAATTCTGTTAGCAACAGCAAGGGGCCGAAAGCACAGACTGCAGAAGATGGTTGCAAGCATGGGA CACTGATGGAGTTCTCCCTTAAAGACCTCTATGCCATCCGAGAGATCCAGGCTGAAGAGAACCTGCTCAA GCTCGTTGTCAACTCACTCTGTCCTGTCATTTTTGGTCATGAACTCGTCAAAGCAGGTCTGACGTTAGCA CTCTTTGGCGGTAGCCAGAAGTACGCAGATGACAAAAACAGAATTCCCATTCGAGGAGACCCACATGTCC TGATTGTTGGAGATCCAGGCTTGGGGAAGAGTCAGATGCTACAGGCAGCATGCAACGTGGCGCCACGTGG TGTGTATGTTTGTGGAAATACCACCACCAGCTCTGGTCTCACTGTGACTCTTTCAAAGGACAGTTCCTCT GGAGATTTTGCTTTGGAAGCTGGTGCCCTTGTACTTGGTGACCAAGGCATCTGTGGAATAGATGAATTTG ATAAAATGGGGAACCAACATCAAGCCCTGTTAGAAGCCATGGAACAGCAGAGTATTAGCCTTGCGAAGGC TGGGGTAGTTTGCAGCCTCCCTGCAAGAACTTCCATTGTTGCTGCTGCAAATCCAGTCGGAGGACACTAC AATAAAGCCAGAACGGTTTCTGAGAATTTAAAGATGGGGAGTGCCCTACTGTCCAGATTCGATTTGGTCT TTATCTTGTTAGATACGCCAAATGAACAGCATGACCACTTACTTTCTGAACATGTGATCGCGATAAGAGC TGGGAAGCAGAAAGCGGTTAGCAGTGCCACAGTCACTCGTGTGCTCAGTCAAGACTCAAATACTTCTGTA CTTGAAGTGGTTTCTGAGAAACCATTATCAGAAAGACTAAAGGTGGCTCCTGGAGAACAGACAGATCCGA TTCCACATCAGCTGTTGAGGAAGTACATTGGCTATGCACGGCAGTATGTCCACCCAAGGTTATCTACGGA CGCTGCTCAGGCCCTTCAGGATTTCTACCTGGAGCTCCGCAAGCAGAGCCAGCGCGTGGGCAGCTCACCC ATCACCACTCGGCAGCTGGAGTCTTTGATCCGTCTGACAGAGGCACGGGCAAGGTTAGAATTGAGAGAGG AAGCAACTAGAGAAGATGCTGAAGATATAATTGAAATTATGAAGCATAGCATGCTAGGAACTTACTCAGA TGAATTCGGAAACCTGGACTTTGAGCGATCCCAGCATGGCTCTGGGATGAGCAACAGGTCAACAGCAAAA AGATTTATTTCTGCTCTCAACAGCATTGCTGAAAGAACTTATAACAACATATTTCAATATCATCAACTTC GTCAGATTGCTAAAGAACTAAACATTCAGGTTGCCGATTTTGAAAACTTCATTGGATCACTGAATGACCA AGGTTATCTCTTGAAGAAAGGCCCAAAGATTTACCAACTTCAAACTATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | BC046780 |
| Insert Size | 2502 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC046780, AAH46780 |
| RefSeq Size | 2908 bp |
| RefSeq ORF | 2501 bp |
| Locus ID | 66634 |
| Gene Summary | Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity. Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs. The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression. However, may play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC) (By similarity). Probably by regulating HR, plays a key role during gametogenesis (PubMed:22771120). Stabilizes MCM9 protein (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210873 | Mcm8 (tGFP-tagged) - Mouse minichromosome maintenance deficient 8 (S |
CNY 5,230.00 |
|
| MR210873 | Mcm8 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 8 (S |
CNY 6,104.00 |
|
| MR210873L3 | Lenti ORF clone of Mcm8 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 8 (S |
CNY 6,650.00 |
|
| MR210873L4 | Lenti ORF clone of Mcm8 (mGFP-tagged) - Mouse minichromosome maintenance deficient 8 (S |
CNY 6,650.00 |
