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Serpina5 (NM_172953) Mouse Tagged ORF Clone
CAT＃: MR206361
- TrueORF®
Serpina5 (Myc-DDK-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade A, member 5 (Serpina5)
ORF Plasmid: tGFP
"NM_172953" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4933415L04; PAI-3; Pci |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206361 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGTTCTTCCCCATTCTGTGCCTGGTGCTGTTCATCAGCCATGGGGTGGCTTCCCGCCGACACTCCC ATTCCAAGAAGAAGAAGGCTAAAGAGTCCTCGGTGGGTGCTGTGGGACCTCCCAGTAGCAAAGACTTTGC TTTCAGACTCTACAGGGCCTTGGCTTCTGAATCCCCTGGTCAGAATGTCTTCTTCTCCCCCTTGAGCGTG TCTATGAGTTTGGGCATGCTCTCCCTGGGGGCTGGCTTGAAGACGAAGACCCAGATCCTAGATGGCCTAG GCCTCAGCCTCCAGCAAGGCCAAGAAGACAAGCTCCACAAGGGCTTCCAACAGCTGCTACAGAGATTCAG GCAGCCTAGTGATGGCCTGCAGCTGAGCCTGGGCAGTGCCCTTTTTAAAGACCCAGCAGTACATATCCGG GACGACTTCCTGAGTGCCATGAAGACACTGTACATGTCAGACACTTTCTCTACCAACTTTGGGAACCCTG AAATTGCCAAGAAGCAGATCAACAACTATGTAGCCAAGCAGACCAAGGGCAAGATTGTAGACTTGATCAA GGATCTTGACAGCACCCATGTCATGATAGTGGTAAATTACATCTTCTTCAAAGCCAAGTGGCAGACGGCC TTCAGTGAGACAAACACCCACAAGATGGATTTCCATGTGACCCCCAAAAAGACCATACGGGTGCCCATGA TGAACCGTGAAGATGAGTATTCCTACTACCTGGACCAAAACATCTCCTGCACGGTGGTGGGGATCCCTTA TCAAGGCAATGCCATTGCTCTATTTATTCTCCCCAGCGAGGGCAAGATGAAGCAGGTGGAGGATGGCCTG GACGAGAGAACATTGAGGAACTGGCTTAAGATGTTCACCAAGAGACGCTTAGATCTTTACCTCCCCAAGT TCTCCATTGAGGCTACCTACAAACTGGAAAATGTCCTCCCCAAGCTGGGCATCCAGGACGTCTTCACCAC CCATGCTGACTTGTCTGGCATTACTGACCATACCAATATCAAGTTGTCTGAGATGGTGCACAAATCCATG ATGGAGGTTGAAGAGTCAGGAACCACAGCAGCTGCCATCACAGGAGCCATCTTCACATTCAGATCTGCTC GGCCGAGCTCCCTGAAGATAGAATTCACCAGACCCTTTCTGCTGACCCTTATGGAGGATTCACATATACT TTTCGTTGGCAAGGTGACCCGGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206361 protein sequence
Red=Cloning site Green=Tags(s) MRFFPILCLVLFISHGVASRRHSHSKKKKAKESSVGAVGPPSSKDFAFRLYRALASESPGQNVFFSPLSV SMSLGMLSLGAGLKTKTQILDGLGLSLQQGQEDKLHKGFQQLLQRFRQPSDGLQLSLGSALFKDPAVHIR DDFLSAMKTLYMSDTFSTNFGNPEIAKKQINNYVAKQTKGKIVDLIKDLDSTHVMIVVNYIFFKAKWQTA FSETNTHKMDFHVTPKKTIRVPMMNREDEYSYYLDQNISCTVVGIPYQGNAIALFILPSEGKMKQVEDGL DERTLRNWLKMFTKRRLDLYLPKFSIEATYKLENVLPKLGIQDVFTTHADLSGITDHTNIKLSEMVHKSM MEVEESGTTAAAITGAIFTFRSARPSSLKIEFTRPFLLTLMEDSHILFVGKVTRP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172953 |
| ORF Size | 1218 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172953.1, NM_172953.2, NM_172953.3, NP_766541.1, NP_766541.2 |
| RefSeq Size | 2162 bp |
| RefSeq ORF | 1218 bp |
| Locus ID | 268591 |
| UniProt ID | P70458 |
| MW | 45.6 kDa |
| Gene Summary | Heparin-dependent serine protease inhibitor acting in body fluids and secretions. Inactivates serine proteases by binding irreversibly to their serine activation site. Involved in the regulation of intravascular and extravascular proteolytic activities. Plays hemostatic roles in the blood plasma. Acts as a procoagulant and proinflammatory factor by inhibiting the anticoagulant activated protein C factor as well as the generation of activated protein C factor by the thrombin/thrombomodulin complex. Acts as an anticoagulant factor by inhibiting blood coagulation factors like prothrombin, factor XI, factor Xa, plasma kallikrein and fibrinolytic enzymes such as tissue- and urinary-type plasminogen activators. In seminal plasma, inactivates several serine proteases implicated in the reproductive system. Inhibits the serpin acrosin; indirectly protects component of the male genital tract from being degraded by excessive released acrosin. Inhibits tissue-and urinary-type plasminogen activator, prostate-specific antigen and kallikrein activities; has a control on the sperm motility and fertilization. Inhibits the activated protein C-catalyzed degradation of SEMG1 and SEMG2; regulates the degradation of semenogelin during the process of transfer of spermatozoa from the male reproductive tract into the female tract. In urine, inhibits urinary-type plasminogen activator and kallikrein activities. Inactivates membrane-anchored serine proteases activities such as MPRSS7 and TMPRSS11E. Inhibits urinary-type plasminogen activator-dependent tumor cell invasion and metastasis. May also play a non-inhibitory role in seminal plasma and urine as a hydrophobic hormone carrier by its binding to retinoic acid (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC214265 | Serpina5 (untagged) - Mouse serine (or cysteine) peptidase inhibitor, clade A, member 5 (Serpina5), (10ug) |
CNY 3,990.00 |
|
| MG206361 | Serpina5 (tGFP-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade A, member 5 (Serpina5) |
CNY 3,140.00 |
|
| MR206361L3 | Lenti ORF clone of Serpina5 (Myc-DDK-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade A, member 5 (Serpina5) |
CNY 4,750.00 |
|
| MR206361L4 | Lenti ORF clone of Serpina5 (mGFP-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade A, member 5 (Serpina5) |
CNY 4,750.00 |
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