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E2f6 (BC019166) Mouse Tagged ORF Clone
CAT＃: MR202901
- TrueORF®
E2f6 (Myc-DDK-tagged) - Mouse E2F transcription factor 6 (cDNA clone MGC:29087 IMAGE:4236246)
ORF Plasmid: tGFP
"BC019166" in other vectors (4)
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CNY 2,380.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EMA, E2F6b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR202901 representing BC019166
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCATCAAAAATAAGGATTAATCTAGAAGAAAATGTACAGTATGTGTCCATGAGAAAAGCTCTGAAAG TGAAGAGGCCCCGGTTTGATGTGTCACTGGTATACTTAACTCGGAAGTTTATGGATCTCGTCAGATCTGC CCCTGGGGGCATTCTTGACTTAAACAAAGTTGCCACAAAACTGGGTGTTCGGAAGAGGCGAGTGTATGAC ATCACCAATGTCTTGGATGGCATCGAACTGGTGGAAAAGAAATCTAAGAACCACATTCGGTGGATAGGAT CTGACCTGAACAACTTTGGGGCCGCACCCCAGCAGAAGAAGCTGCAGGCAGAGCTCTCCGACCTGTCGGC CATGGAAGACGCCTTGGACGAGTTGATTAAAGATTGTGCTCAGCAACTGTTGGAGTTAACAGATGACAAG GAAAATGAAAGACTAGCGTATGTAACCTATCAGGATATTCACGGCATTCAAGCTTTCCATGAACAGATTG TCATTGCAGTGAAGGCTCCAGAGGAAACCAGACTGGATGTTCCAGCTCCCAGAGAAGATTCTATCACAGT ACATATTAGGAGCACCAAAGGACCCATTGATGTATATTTGTGTGAAGTAGAACAGAACCATTCAAATGGT AAAACCAATGATGGAATAGGAGCCTCTCCATCTAAAAGCAGCCATCCACAATGCCCAGAGAAAGAAGACG AGCCTCCTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202901 representing BC019166
Red=Cloning site Green=Tags(s) MPSKIRINLEENVQYVSMRKALKVKRPRFDVSLVYLTRKFMDLVRSAPGGILDLNKVATKLGVRKRRVYD ITNVLDGIELVEKKSKNHIRWIGSDLNNFGAAPQQKKLQAELSDLSAMEDALDELIKDCAQQLLELTDDK ENERLAYVTYQDIHGIQAFHEQIVIAVKAPEETRLDVPAPREDSITVHIRSTKGPIDVYLCEVEQNHSNG KTNDGIGASPSKSSHPQCPEKEDEPPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC019166 |
| ORF Size | 711 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC019166.1 |
| RefSeq Size | 2506 bp |
| RefSeq ORF | 713 bp |
| Locus ID | 50496 |
| MW | 91.9 kDa |
| Gene Summary | Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218297 | E2f6 (untagged) - Mouse E2F transcription factor 6 (cDNA clone MGC:29087 IMAGE:4236246), (10ug) |
CNY 6,940.00 |
|
| MG202901 | E2f6 (tGFP-tagged) - Mouse E2F transcription factor 6 (cDNA clone MGC:29087 IMAGE:4236246) |
CNY 2,660.00 |
|
| MR202901L3 | Lenti ORF clone of E2f6 (Myc-DDK-tagged) - Mouse E2F transcription factor 6 (cDNA clone MGC:29087 IMAGE:4236246) |
CNY 4,280.00 |
|
| MR202901L4 | Lenti ORF clone of E2f6 (mGFP-tagged) - Mouse E2F transcription factor 6 (cDNA clone MGC:29087 IMAGE:4236246) |
CNY 4,280.00 |
