United States
Germany
Japan
United Kingdom
China
E2f6 (BC019166) Mouse Untagged Clone
CAT＃: MC218297
E2f6 (untagged) - Mouse E2F transcription factor 6 (cDNA clone MGC:29087 IMAGE:4236246), (10ug)
CN¥ 6,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | EMA, E2F6b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC019166
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCATCAAAAATAAGGATTAATCTAGAAGAAAATGTACAGTATGTGTCCATGAGAAAAGCTCTGAAAG TGAAGAGGCCCCGGTTTGATGTGTCACTGGTATACTTAACTCGGAAGTTTATGGATCTCGTCAGATCTGC CCCTGGGGGCATTCTTGACTTAAACAAAGTTGCCACAAAACTGGGTGTTCGGAAGAGGCGAGTGTATGAC ATCACCAATGTCTTGGATGGCATCGAACTGGTGGAAAAGAAATCTAAGAACCACATTCGGTGGATAGGAT CTGACCTGAACAACTTTGGGGCCGCACCCCAGCAGAAGAAGCTGCAGGCAGAGCTCTCCGACCTGTCGGC CATGGAAGACGCCTTGGACGAGTTGATTAAAGATTGTGCTCAGCAACTGTTGGAGTTAACAGATGACAAG GAAAATGAAAGACTAGCGTATGTAACCTATCAGGATATTCACGGCATTCAAGCTTTCCATGAACAGATTG TCATTGCAGTGAAGGCTCCAGAGGAAACCAGACTGGATGTTCCAGCTCCCAGAGAAGATTCTATCACAGT ACATATTAGGAGCACCAAAGGACCCATTGATGTATATTTGTGTGAAGTAGAACAGAACCATTCAAATGGT AAAACCAATGATGGAATAGGAGCCTCTCCATCTAAAAGCAGCCATCCACAATGCCCAGAGAAAGAAGACG AGCCTCCTCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | BC019166 |
| Insert Size | 714 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC019166, AAH19166 |
| RefSeq Size | 2506 bp |
| RefSeq ORF | 713 bp |
| Locus ID | 50496 |
| Gene Summary | Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG202901 | E2f6 (tGFP-tagged) - Mouse E2F transcription factor 6 (cDNA clone MGC:29087 IMAGE:4236246) |
CN¥ 2,660.00 |
|
| MR202901 | E2f6 (Myc-DDK-tagged) - Mouse E2F transcription factor 6 (cDNA clone MGC:29087 IMAGE:4236246) |
CN¥ 2,380.00 |
|
| MR202901L3 | Lenti ORF clone of E2f6 (Myc-DDK-tagged) - Mouse E2F transcription factor 6 (cDNA clone MGC:29087 IMAGE:4236246) |
CN¥ 4,280.00 |
|
| MR202901L4 | Lenti ORF clone of E2f6 (mGFP-tagged) - Mouse E2F transcription factor 6 (cDNA clone MGC:29087 IMAGE:4236246) |
CN¥ 4,280.00 |
