Mink1 (BC052474) Mouse Tagged ORF Clone

CAT#: MG211931

  • TrueORF®

Mink1 (tGFP-tagged) - Mouse misshapen-like kinase 1 (zebrafish) (cDNA clone MGC:63403 IMAGE:6833893)

ORF Plasmid: DDK tGFP



  "BC052474" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 8,360.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms B55, MINK, RP23-122P1.6
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211931 representing BC052474
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCGACCCAGCCCCCGCCCGCAGCCTGGACGACATCGACCTGTCTGCCCTGCGGGACCCTGCAGGAA
TCTTTGAGCTGGTGGAGGTGGTTGGCAATGGAACCTATGGACAGGTATACAAGGGGCGGCACGTCAAGAC
TGGGCAGCTGGCTGCCATTAAGGTCATGGATGTCACAGAGGATGAGGAGGAAGAGATCAAACAGGAAATC
AACATGTTAAAGAAGTACTCTCACCATCGCAATATTGCCACCTACTATGGGGCCTTTATCAAGAAGAGCC
CTCCTGGGAACGATGACCAGCTCTGGCTGGTGATGGAGTTCTGCGGTGCTGGTTCAGTGACCGACCTGGT
AAAGAACACAAAAGGGAACGCACTGAAGGAGGATTGCATTGCTTACATCTGCAGGGAGATTCTCAGGGGT
CTTGCCCATCTCCATGCCCACAAGGTGATCCACAGAGATATCAAGGGACAAAATGTGCTGCTGACAGAGA
ATGCTGAAGTCAAGCTAGTGGATTTTGGGGTGAGTGCTCAGCTGGACCGCACTGTGGGCAGGCGGAACAC
TTTCATTGGAACCCCATACTGGATGGCTCCAGAAGTCATTGCCTGTGACGAGAACCCCGATGCCACCTAT
GACTACAGGAGTGACATTTGGTCTCTAGGAATCACAGCCATTGAAATGGCAGAGGGAGCCCCCCCTCTGT
GTGACATGCACCCTATGCGGGCCCTCTTCCTCATCCCTCGGAACCCTCCCCCCAGGCTCAAGTCAAAGAA
ATGGTCTAAGAAGTTCACTGACTTCATCGACACGTGTCTCATCAAGACTTACCTGAGCCGCCCACCCACC
GAACAGTTACTCAAATTCCCCTTCATCCGAGACCAGCCCACGGAGCGGCAGGTCCGCATCCAGCTCAAGG
ACCACATCGACCGCTCGCGGAAGAAGCGGGAGGAGACAGAGTATGAGTACAGCGGCAGTGAGGAGGAAGA
CGACAGCCATGGAGAGGAAGGCGAGCCAAGCTCCATCATGAATGTGCCCGGTGAGTCCACACTGCGCAGA
GAATTCCTCAGACTCCAGCAGGAGAATAAGAGCAACTCTGAGGCTTTAAAGCAGCAGCAGCAGCTGCAGC
AACAGCAGCAGCGGGACCCGGAGGCACACATCAAACACCTGCTGCACCAGCGGCAGCGTCGCATAGAGGA
GCAGAAGGAGGAGCGGCGACGTGTGGAGGAGCAACAGCGGCGAGAGCGAGAACAGCGTAAGCTACAAGAG
AAGGAGCAGCAGCGGCGATTGGAAGACATGCAAGCCCTACGACGAGAGGAAGAGAGGCGGCAAGCAGAGC
GGGAACAGGAATACAAGCGGAAGCAGCTGGAGGAGCAGCGGCAGTCAGAGCGGCTGCAGAGACAGCTGCA
GCAGGAGCACGCCTACCTCAAGTCCCTGCAGCAGCAGCAGCAGCAGCAGCAGCTCCAGAAGCAGCAGCAG
CAGCAGCAGCAGATCCTGCCTGGAGACAGGAAGCCCCTGTATCATTACGGTCGGGGCATTAATCCTGCTG
ACAAGCCAGCATGGGCCCGCGAGGTGGAAGAGAGAGCACGGATGAACAAGCAGCAGAACTCTCCCTTGGC
GAAGGCGAAGCCAAGCAGTGCGGGGCCAGAGCCCCCCATCTCCCAGGCCTCTCCTAGCCCCCCAGGACCT
CTTTCCCAGACTCCTCCTATGCAGAGGCCTGTGGAGCCCCAGGAAGGACCGCACAAGAGCCTGGTGGCAC
ACCGGGTCCCACTGAAGCCATATGCAGCACCTGTACCCCGATCCCAGTCCCTGCAGGACCAGCCGACTCG
AAACCTGGCTGCCTTCCCAGCCTCCCACGACCCTGACCCTGCTGCTGTCCCTACACCCACTGCCACACCC
AGTGCCCGAGGAGCTGTCATCCGCCAGAATTCAGACCCCACCTCTGAAGGGCCAGGGCCTAGCCCAAACC
CTCCATCCTGGGTTCGGCCTGATAATGAGGCTCCACCTAAGGTTCCACAGAGGACCTCTTCTATCGCCAC
TGCCCTTAACACCAGTGGGGCCGGAGGGTCCCGGCCAGCTCAGGCTGTCCGTGCCAGACCTCGCAGTAAC
TCCGCCTGGCAAATCTATCTGCAGAGGCGGGCAGAGCGGGGCACCCCCAAGCCTCCCGGGCCCCCAGCTC
AGCCCCCTGGCCCGCCCAACGCCTCTAGTAACCCTGACCTCAGGAGGAGTGACCCTGGCTGGGAGCGCTC
AGACAGTGTCCTCCCGGCCTCCCACGGCCACCTCCCTCAGGCTGGCTCCTTGGAGCGGAACCGAAACCGT
GTGGGAGCCTCCACAAAACTGGATAGCTCTCCAGTGCTCTCCCCTGGGAACAAAGCCAAGCCTGAAGACC
ACCGCTCAAGGCCAGGCCGGCCCGCAGACTTTGTGTTGCTCAAAGAGCGGACTCTGGATGAGGCCCCTAA
GCCTCCCAAGAAGGCCATGGACTACTCCTCATCCAGTGAGGAGGTGGAAAGCAGTGAAGAGGAGGAGGAG
GAAGGCGATGGGGAGCCGTCAGAGGGGAGCAGAGACACTCCCGGGGGCCGCAGTGATGGTGATACAGACA
GCGTCAGCACCATGGTGGTTCATGATGTTGAGGAGATATCCGGGACCCAGCCCTCATATGGCGGCGGCAC
CATGGTGGTCCAGCGTACTCCTGAAGAGGAACGAAGCCTGCTGCTTGCTGATAGCAATGGCTACACAAAC
CTGCCTGATGTGGTCCAGCCCAGCCACTCACCTACTGAGAACAGCAAAGGTCAAAGCCCTCCAACAAAGG
ATGGAGGCAGTGATTACCAGTCTCGTGGGCTGGTAAAGGCCCCAGGAAAGAGCTCATTCACCATGTTTGT
GGATCTAGGGATCTACCAGCCTGGAGGCAGTGGGGACACCATCCCTATCACAGCCCTAGTGGGTGGAGAA
GGTGGTCGCCTTGATCAACTGCAGTTCGATGTGAGGAAGGGCTCTGTGGTCAACGTCAATCCCACCAACA
CCCGAGCTCATAGTGAAACTCCTGAAATTCGCAAGTACAAGAAGCGATTCAACTCAGAGATCCTATGTGC
AGCTCTCTGGGGGGTCAACCTCCTAGTGGGCACAGAGAATGGGCTGATGTTGCTGGACCGAAGTGGGCAG
GGCAAGGTGTATGGACTTATTGGGCGACGACGCTTCCAGCAAATGGATGTCTTAGAAGGGCTCAACTTGC
TCATCACCATCTCAGGGAAAAGGAACAAACTGCGGGTATATTACCTGTCCTGGCTTCGGAACAAGATCCT
ACACAATGACCCAGAGGTGGAAAAGAAGCAGGGGTGGACCACCGTGGGGGACATGGAGGGCTGCGGCCAC
TACCGTGTTGTGAAATATGAACGGATTAAGTTCCTGGTCATTGCCCTGAAGAACTACGTGGAGGTTTATG
CCTGGGCTCCCAAACCCTACCACAAATTCATGGCCTTCAAGTCCTTTGCTGACCTCCCTCACCGCCCTCT
ACTGGTGGACCTGACAGTAGAGGAGGGACAGCGGCTCAAGGTCATCTATGGCTCCAGTGCTGGCTTCCAT
GCTGTGGATGTTGATTCTGGGAACAGCTATGACATCTACATCCCTGTACATATCCAGAGCCAGATCACAC
CCCACGCCATCATCTTCCTCCCCAACACTGATGGCATGGAGATGCTGCTGTGCTATGAAGATGAGGGTGT
CTATGTCAACACTTACGGGCGGATCATCAAGGATGTGGTGCTGCAGTGGGGAGAGATGCCCACCTCTGTG
GCCTACATCTGCTCCAACCAGATAATGGGCTGGGGTGAGAAGGCCATAGAGATCCGCTCTGTGGAGACAG
GCCACCTAGATGGGGTCTTCATGCACAAACGAGCCCAGAGGCTCAAGTTCCTGTGTGAGCGCAATGACAA
GGTGTTTTTTGCCTCTGTCCGCTCTGGAGGAAGCAGCCAAGTTTACTTTATGACTCTGAACCGTAACTGC
ATCATGAACTGG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211931 representing BC052474
Red=Cloning site Green=Tags(s)

MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEI
NMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRG
LAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATY
DYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPT
EQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKREETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRR
EFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQE
KEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQ
QQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGP
LSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATP
SARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSN
SAWQIYLQRRAERGTPKPPGPPAQPPGPPNASSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNR
VGASTKLDSSPVLSPGNKAKPEDHRSRPGRPADFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEE
EGDGEPSEGSRDTPGGRSDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTN
LPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGE
GGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQ
GKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGH
YRVVKYERIKFLVIALKNYVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFH
AVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSV
AYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNC
IMNW

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC052474
ORF Size 4004 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC052474, AAH52474
RefSeq Size 4842 bp
RefSeq ORF 4004 bp
Locus ID 50932
Gene Summary Serine/threonine kinase which acts as a negative regulator of Ras-related Rap2-mediated signal transduction to control neuronal structure and AMPA receptor trafficking. Required for normal synaptic density, dendrite complexity, as well as surface AMPA receptor expression in hippocampal neurons. Can activate the JNK and MAPK14/p38 pathways and mediates stimulation of the stress-activated protein kinase MAPK14/p38 MAPK downstream of the Raf/ERK pathway. Phosphorylates: TANC1 upon stimulation by RAP2A, MBP and SMAD1. Has an essential function in negative selection of thymocytes, perhaps by coupling NCK1 to activation of JNK1.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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