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Mink1 (BC052474) Mouse Untagged Clone
CAT＃: MC224580
Mink1 (untagged) - Mouse misshapen-like kinase 1 (zebrafish) (cDNA clone MGC:63403 IMAGE:6833893), (10ug)
CNY 19,190.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | B55, MINK, RP23-122P1.6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC052474
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGACCCAGCCCCCGCCCGCAGCCTGGACGACATCGACCTGTCTGCCCTGCGGGACCCTGCAGGAA TCTTTGAGCTGGTGGAGGTGGTTGGCAATGGAACCTATGGACAGGTATACAAGGGGCGGCACGTCAAGAC TGGGCAGCTGGCTGCCATTAAGGTCATGGATGTCACAGAGGATGAGGAGGAAGAGATCAAACAGGAAATC AACATGTTAAAGAAGTACTCTCACCATCGCAATATTGCCACCTACTATGGGGCCTTTATCAAGAAGAGCC CTCCTGGGAACGATGACCAGCTCTGGCTGGTGATGGAGTTCTGCGGTGCTGGTTCAGTGACCGACCTGGT AAAGAACACAAAAGGGAACGCACTGAAGGAGGATTGCATTGCTTACATCTGCAGGGAGATTCTCAGGGGT CTTGCCCATCTCCATGCCCACAAGGTGATCCACAGAGATATCAAGGGACAAAATGTGCTGCTGACAGAGA ATGCTGAAGTCAAGCTAGTGGATTTTGGGGTGAGTGCTCAGCTGGACCGCACTGTGGGCAGGCGGAACAC TTTCATTGGAACCCCATACTGGATGGCTCCAGAAGTCATTGCCTGTGACGAGAACCCCGATGCCACCTAT GACTACAGGAGTGACATTTGGTCTCTAGGAATCACAGCCATTGAAATGGCAGAGGGAGCCCCCCCTCTGT GTGACATGCACCCTATGCGGGCCCTCTTCCTCATCCCTCGGAACCCTCCCCCCAGGCTCAAGTCAAAGAA ATGGTCTAAGAAGTTCACTGACTTCATCGACACGTGTCTCATCAAGACTTACCTGAGCCGCCCACCCACC GAACAGTTACTCAAATTCCCCTTCATCCGAGACCAGCCCACGGAGCGGCAGGTCCGCATCCAGCTCAAGG ACCACATCGACCGCTCGCGGAAGAAGCGGGAGGAGACAGAGTATGAGTACAGCGGCAGTGAGGAGGAAGA CGACAGCCATGGAGAGGAAGGCGAGCCAAGCTCCATCATGAATGTGCCCGGTGAGTCCACACTGCGCAGA GAATTCCTCAGACTCCAGCAGGAGAATAAGAGCAACTCTGAGGCTTTAAAGCAGCAGCAGCAGCTGCAGC AACAGCAGCAGCGGGACCCGGAGGCACACATCAAACACCTGCTGCACCAGCGGCAGCGTCGCATAGAGGA GCAGAAGGAGGAGCGGCGACGTGTGGAGGAGCAACAGCGGCGAGAGCGAGAACAGCGTAAGCTACAAGAG AAGGAGCAGCAGCGGCGATTGGAAGACATGCAAGCCCTACGACGAGAGGAAGAGAGGCGGCAAGCAGAGC GGGAACAGGAATACAAGCGGAAGCAGCTGGAGGAGCAGCGGCAGTCAGAGCGGCTGCAGAGACAGCTGCA GCAGGAGCACGCCTACCTCAAGTCCCTGCAGCAGCAGCAGCAGCAGCAGCAGCTCCAGAAGCAGCAGCAG CAGCAGCAGCAGATCCTGCCTGGAGACAGGAAGCCCCTGTATCATTACGGTCGGGGCATTAATCCTGCTG ACAAGCCAGCATGGGCCCGCGAGGTGGAAGAGAGAGCACGGATGAACAAGCAGCAGAACTCTCCCTTGGC GAAGGCGAAGCCAAGCAGTGCGGGGCCAGAGCCCCCCATCTCCCAGGCCTCTCCTAGCCCCCCAGGACCT CTTTCCCAGACTCCTCCTATGCAGAGGCCTGTGGAGCCCCAGGAAGGACCGCACAAGAGCCTGGTGGCAC ACCGGGTCCCACTGAAGCCATATGCAGCACCTGTACCCCGATCCCAGTCCCTGCAGGACCAGCCGACTCG AAACCTGGCTGCCTTCCCAGCCTCCCACGACCCTGACCCTGCTGCTGTCCCTACACCCACTGCCACACCC AGTGCCCGAGGAGCTGTCATCCGCCAGAATTCAGACCCCACCTCTGAAGGGCCAGGGCCTAGCCCAAACC CTCCATCCTGGGTTCGGCCTGATAATGAGGCTCCACCTAAGGTTCCACAGAGGACCTCTTCTATCGCCAC TGCCCTTAACACCAGTGGGGCCGGAGGGTCCCGGCCAGCTCAGGCTGTCCGTGCCAGACCTCGCAGTAAC TCCGCCTGGCAAATCTATCTGCAGAGGCGGGCAGAGCGGGGCACCCCCAAGCCTCCCGGGCCCCCAGCTC AGCCCCCTGGCCCGCCCAACGCCTCTAGTAACCCTGACCTCAGGAGGAGTGACCCTGGCTGGGAGCGCTC AGACAGTGTCCTCCCGGCCTCCCACGGCCACCTCCCTCAGGCTGGCTCCTTGGAGCGGAACCGAAACCGT GTGGGAGCCTCCACAAAACTGGATAGCTCTCCAGTGCTCTCCCCTGGGAACAAAGCCAAGCCTGAAGACC ACCGCTCAAGGCCAGGCCGGCCCGCAGACTTTGTGTTGCTCAAAGAGCGGACTCTGGATGAGGCCCCTAA GCCTCCCAAGAAGGCCATGGACTACTCCTCATCCAGTGAGGAGGTGGAAAGCAGTGAAGAGGAGGAGGAG GAAGGCGATGGGGAGCCGTCAGAGGGGAGCAGAGACACTCCCGGGGGCCGCAGTGATGGTGATACAGACA GCGTCAGCACCATGGTGGTTCATGATGTTGAGGAGATATCCGGGACCCAGCCCTCATATGGCGGCGGCAC CATGGTGGTCCAGCGTACTCCTGAAGAGGAACGAAGCCTGCTGCTTGCTGATAGCAATGGCTACACAAAC CTGCCTGATGTGGTCCAGCCCAGCCACTCACCTACTGAGAACAGCAAAGGTCAAAGCCCTCCAACAAAGG ATGGAGGCAGTGATTACCAGTCTCGTGGGCTGGTAAAGGCCCCAGGAAAGAGCTCATTCACCATGTTTGT GGATCTAGGGATCTACCAGCCTGGAGGCAGTGGGGACACCATCCCTATCACAGCCCTAGTGGGTGGAGAA GGTGGTCGCCTTGATCAACTGCAGTTCGATGTGAGGAAGGGCTCTGTGGTCAACGTCAATCCCACCAACA CCCGAGCTCATAGTGAAACTCCTGAAATTCGCAAGTACAAGAAGCGATTCAACTCAGAGATCCTATGTGC AGCTCTCTGGGGGGTCAACCTCCTAGTGGGCACAGAGAATGGGCTGATGTTGCTGGACCGAAGTGGGCAG GGCAAGGTGTATGGACTTATTGGGCGACGACGCTTCCAGCAAATGGATGTCTTAGAAGGGCTCAACTTGC TCATCACCATCTCAGGGAAAAGGAACAAACTGCGGGTATATTACCTGTCCTGGCTTCGGAACAAGATCCT ACACAATGACCCAGAGGTGGAAAAGAAGCAGGGGTGGACCACCGTGGGGGACATGGAGGGCTGCGGCCAC TACCGTGTTGTGAAATATGAACGGATTAAGTTCCTGGTCATTGCCCTGAAGAACTACGTGGAGGTTTATG CCTGGGCTCCCAAACCCTACCACAAATTCATGGCCTTCAAGTCCTTTGCTGACCTCCCTCACCGCCCTCT ACTGGTGGACCTGACAGTAGAGGAGGGACAGCGGCTCAAGGTCATCTATGGCTCCAGTGCTGGCTTCCAT GCTGTGGATGTTGATTCTGGGAACAGCTATGACATCTACATCCCTGTACATATCCAGAGCCAGATCACAC CCCACGCCATCATCTTCCTCCCCAACACTGATGGCATGGAGATGCTGCTGTGCTATGAAGATGAGGGTGT CTATGTCAACACTTACGGGCGGATCATCAAGGATGTGGTGCTGCAGTGGGGAGAGATGCCCACCTCTGTG GCCTACATCTGCTCCAACCAGATAATGGGCTGGGGTGAGAAGGCCATAGAGATCCGCTCTGTGGAGACAG GCCACCTAGATGGGGTCTTCATGCACAAACGAGCCCAGAGGCTCAAGTTCCTGTGTGAGCGCAATGACAA GGTGTTTTTTGCCTCTGTCCGCTCTGGAGGAAGCAGCCAAGTTTACTTTATGACTCTGAACCGTAACTGC ATCATGAACTGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | BC052474 |
| Insert Size | 4005 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC052474, AAH52474 |
| RefSeq Size | 4842 bp |
| RefSeq ORF | 4004 bp |
| Locus ID | 50932 |
| Gene Summary | Serine/threonine kinase which acts as a negative regulator of Ras-related Rap2-mediated signal transduction to control neuronal structure and AMPA receptor trafficking. Required for normal synaptic density, dendrite complexity, as well as surface AMPA receptor expression in hippocampal neurons. Can activate the JNK and MAPK14/p38 pathways and mediates stimulation of the stress-activated protein kinase MAPK14/p38 MAPK downstream of the Raf/ERK pathway. Phosphorylates: TANC1 upon stimulation by RAP2A, MBP and SMAD1. Has an essential function in negative selection of thymocytes, perhaps by coupling NCK1 to activation of JNK1.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211931 | Mink1 (tGFP-tagged) - Mouse misshapen-like kinase 1 (zebrafish) (cDNA clone MGC:63403 IMAGE:6833893) |
CNY 8,360.00 |
|
| MR211931 | Mink1 (Myc-DDK-tagged) - Mouse misshapen-like kinase 1 (zebrafish) (cDNA clone MGC:63403 IMAGE:6833893) |
CNY 9,128.00 |
|
| MR211931L3 | Lenti ORF clone of Mink1 (Myc-DDK-tagged) - Mouse misshapen-like kinase 1 (zebrafish) (cDNA clone MGC:63403 IMAGE:6833893) |
CNY 9,500.00 |
|
| MR211931L4 | Lenti ORF clone of Mink1 (mGFP-tagged) - Mouse misshapen-like kinase 1 (zebrafish) (cDNA clone MGC:63403 IMAGE:6833893) |
CNY 9,500.00 |
