Large1 (NM_010687) Mouse Tagged ORF Clone
CAT#: MG210487
- TrueORF®
Large (tGFP-tagged) - Mouse like-glycosyltransferase (Large)
ORF Plasmid: DDK
"NM_010687" in other vectors (4)
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CNY 6,370.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | BPFD#36; enr; fg; froggy; Gyltl1a; Large; Mbp-1; Mbp1; myd |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG210487 representing NM_010687
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGGAATCTGCAGAGGGAGAAGGAAATTCCTGGCTGCCTCTTTGACTCTACTCTGCATACCAGCCA TCACCTGGATTTACTTGTTTGCTGGGAGCTTTGAAGATGGGAAACCTGTGTCTCTGTCTCCCCTGGAGTC CCAAGCCCACAGTCCCCGGTACACAGCCTCCAGCCAACGGGAGCGGGAGAGCCTGGAAGTGCGTGTTCGA GAAGTTGAGGAGGAAAACCGTGCTCTGCGCAGGCAGCTCAGCCTGGCCCAGGGTCAGAGCCCTGCTCACC ACCGTGGGAACCACTCCAAGACCTACTCCATGGAGGAGGGGACAGGGGACAGTGAGAATCTGCGAGCTGG CATTGTGGCAGGTAACAGCTCTGAGTGTGGGCAGCAGCCAGCTGTGGAAAAGTGTGAGACCATCCATGTG GCCATCGTCTGTGCCGGATACAATGCCAGCAGGGACGTTGTCACCCTGGTGAAATCTGTCCTCTTTCATA GGCGGAACCCTCTGCACTTCCACCTCATTGCTGATTCTATTGCTGAGCAGATCCTGGCTACACTCTTCCA GACCTGGATGGTACCTGCAGTGCGAGTAGACTTCTATAATGCTGATGAGCTCAAGTCTGAAGTGTCCTGG ATTCCCAACAAACATTACTCTGGAATTTATGGCCTGATGAAGCTGGTTCTGACCAAAACTCTGCCTGCCA ACCTGGAGAGAGTCATTGTCCTAGACACCGACATCACCTTTGCAACAGACATTGCAGAGCTGTGGGCCGT GTTCCATAAGTTCAAAGGGCAACAGGTCCTGGGCTCGGTAGAGAACCAGAGTGACTGGTATCTTGGAAAT CTCTGGAAAAACCACCGTCCATGGCCAGCTCTTGGAAGGGGCTATAACACAGGAGTCATACTACTGCTTC TGGACAAGCTTCGGAAGATGAAATGGGAGCAGATGTGGAGACTGACAGCAGAGAGGGAACACATGGGCAT GTTGTCTACATCCTTGGCTGACCAGGATATTTTCAATGCTGTTATCAAACAAAACCCCTTTCTGGTGTAT CAGCTTCCCTGCTTCTGGAATGTGCAGCTGTCAGACCACACCCGCTCTGAGCAGTGCTACAGAGATGTGT CTGATTTAAAGGTCATCCACTGGAACTCCCCCAAGAAGCTCCGTGTGAAGAACAAACATGTGGAATTCTT CCGTAACCTCTACCTGACCTTCTTGGAGTATGATGGCAACCTCCTGCGGCGGGAGCTGTTTGGCTGCCCC AGTGAGACTGATGTCAACAATGAAAATCTTCAGAAGCAGCTGTCTGAGCTGGATGAGGATAACTTGTGCT ATGAGTTCCGGCGAGAGCGCTTCACTGTCCACAGAACCCACCTGTACTTCCTGCACTACGAGTTTGAGCC TTCTGCTGACAACACAGACGTTACCCTTGTTGCTCAGCTCTCCATGGACAGACTACAGATGCTGGAGGCC ATCTGTAAACACTGGGAGGGACCCATCAGCCTGGCCCTCTACCTGTCAGATGCAGAAGCCCAGCAGTTCC TCCGCTATGCCCAGGGATCCGAGGTGCTCATGAGCCGGCAGAACGTGGGCTACCACATCGTGTACAAGGA GGGTCAGTTCTATCCTGTCAATCTTCTGCGAAACGTGGCTATGAAGCACATCAGCACACCCTACATGTTC TTATCTGACATTGACTTCCTGCCTATGTATGGGCTCTATGAGTACCTCAGGAAATCTGTCATCCAGCTTG ACCTTGCCAATACCAAGAAAGCAATGATTGTCCCAGCATTTGAAACGCTGCGCTACAGACTCTCTTTCCC CAAGTCAAAAGCAGAGCTGCTGTCAATGTTGGACATGGGGACCCTATTCACATTCAGGTATCACGTATGG ACTAAAGGCCATGCACCCACAAACTTTGCCAAGTGGCGGACTGCCACCACACCTTACCAGGTTGAGTGGG AGGCTGATTTTGAGCCATATGTTGTTGTGAGACGAGACTGCCCTGAGTATGATCGGAGATTTGTGGGCTT TGGCTGGAACAAAGTGGCTCACATCATGGAATTGGATGCACAGGAGTATGAGTTCACCGTGTTGCCCAAT GCCTACATGATCCACATGCCCCATGCCCCTAGTTTCGACATCACTAAGTTCCGTTCCAACAAGCAATACC GCATCTGTCTCAAAACCCTGAAGGAAGAGTTTCAACAAGACATGTCCCGACGATATGGCTTTGCTGCCTT GGAATATCTCACAGCTGAGAACAACAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210487 representing NM_010687
Red=Cloning site Green=Tags(s) MLGICRGRRKFLAASLTLLCIPAITWIYLFAGSFEDGKPVSLSPLESQAHSPRYTASSQRERESLEVRVR EVEEENRALRRQLSLAQGQSPAHHRGNHSKTYSMEEGTGDSENLRAGIVAGNSSECGQQPAVEKCETIHV AIVCAGYNASRDVVTLVKSVLFHRRNPLHFHLIADSIAEQILATLFQTWMVPAVRVDFYNADELKSEVSW IPNKHYSGIYGLMKLVLTKTLPANLERVIVLDTDITFATDIAELWAVFHKFKGQQVLGSVENQSDWYLGN LWKNHRPWPALGRGYNTGVILLLLDKLRKMKWEQMWRLTAEREHMGMLSTSLADQDIFNAVIKQNPFLVY QLPCFWNVQLSDHTRSEQCYRDVSDLKVIHWNSPKKLRVKNKHVEFFRNLYLTFLEYDGNLLRRELFGCP SETDVNNENLQKQLSELDEDNLCYEFRRERFTVHRTHLYFLHYEFEPSADNTDVTLVAQLSMDRLQMLEA ICKHWEGPISLALYLSDAEAQQFLRYAQGSEVLMSRQNVGYHIVYKEGQFYPVNLLRNVAMKHISTPYMF LSDIDFLPMYGLYEYLRKSVIQLDLANTKKAMIVPAFETLRYRLSFPKSKAELLSMLDMGTLFTFRYHVW TKGHAPTNFAKWRTATTPYQVEWEADFEPYVVVRRDCPEYDRRFVGFGWNKVAHIMELDAQEYEFTVLPN AYMIHMPHAPSFDITKFRSNKQYRICLKTLKEEFQQDMSRRYGFAALEYLTAENNS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010687 |
ORF Size | 2268 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010687.1, NP_034817.1 |
RefSeq Size | 3669 bp |
RefSeq ORF | 2271 bp |
Locus ID | 16795 |
UniProt ID | Q9Z1M7 |
Gene Summary | Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) (PubMed:23125099, PubMed:23135544). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity and plays a key role in skeletal muscle function and regeneration (PubMed:15184894, PubMed:24132234). LARGE elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure initiated by B3GNT1/B4GAT1 by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC221370 | Large (untagged) - Mouse like-glycosyltransferase (Large), (10ug) |
CNY 9,790.00 |
|
MR210487 | Large (Myc-DDK-tagged) - Mouse like-glycosyltransferase (Large) |
CNY 5,536.00 |
|
MR210487L3 | Lenti ORF clone of Large (Myc-DDK-tagged) - Mouse like-glycosyltransferase (Large) |
CNY 7,700.00 |
|
MR210487L4 | Lenti ORF clone of Large (mGFP-tagged) - Mouse like-glycosyltransferase (Large) |
CNY 7,700.00 |