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Large1 (NM_010687) Mouse Untagged Clone
CAT＃: MC221370
Large (untagged) - Mouse like-glycosyltransferase (Large), (10ug)
CNY 9,790.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | BPFD#36; enr; fg; froggy; Gyltl1a; Large; Mbp-1; Mbp1; myd |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221370 representing NM_010687
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGGAATCTGCAGAGGGAGAAGGAAATTCCTGGCTGCCTCTTTGACTCTACTCTGCATACCAGCCA TCACCTGGATTTACTTGTTTGCTGGGAGCTTTGAAGATGGGAAACCTGTGTCTCTGTCTCCCCTGGAGTC CCAAGCCCACAGTCCCCGGTACACAGCCTCCAGCCAACGGGAGCGGGAGAGCCTGGAAGTGCGTGTTCGA GAAGTTGAGGAGGAAAACCGTGCTCTGCGCAGGCAGCTCAGCCTGGCCCAGGGTCAGAGCCCTGCTCACC ACCGTGGGAACCACTCCAAGACCTACTCCATGGAGGAGGGGACAGGGGACAGTGAGAATCTGCGAGCTGG CATTGTGGCAGGTAACAGCTCTGAGTGTGGGCAGCAGCCAGCTGTGGAAAAGTGTGAGACCATCCATGTG GCCATCGTCTGTGCCGGATACAATGCCAGCAGGGACGTTGTCACCCTGGTGAAATCTGTCCTCTTTCATA GGCGGAACCCTCTGCACTTCCACCTCATTGCTGATTCTATTGCTGAGCAGATCCTGGCTACACTCTTCCA GACCTGGATGGTACCTGCAGTGCGAGTAGACTTCTATAATGCTGATGAGCTCAAGTCTGAAGTGTCCTGG ATTCCCAACAAACATTACTCTGGAATTTATGGCCTGATGAAGCTGGTTCTGACCAAAACTCTGCCTGCCA ACCTGGAGAGAGTCATTGTCCTAGACACCGACATCACCTTTGCAACAGACATTGCAGAGCTGTGGGCCGT GTTCCATAAGTTCAAAGGGCAACAGGTCCTGGGCTTGGTAGAGAACCAGAGTGACTGGTATCTTGGAAAT CTCTGGAAAAACCACCGTCCATGGCCAGCTCTTGGAAGGGGCTATAACACAGGAGTCATACTACTGCTTC TGGACAAGCTTCGGAAGATGAAATGGGAGCAGATGTGGAGACTGACAGCAGAGAGGGAACTCATGGGCAT GTTGTCTACATCCTTGGCTGACCAGGATATTTTCAATGCTGTTATCAAACAAAACCCCTTTCTGGTGTAT CAGCTTCCCTGCTTCTGGAATGTGCAGCTGTCAGACCACACCCGCTCTGAGCAGTGCTACAGAGATGTGT CTGATTTAAAGGTCATCCACTGGAACTCCCCCAAGAAGCTCCGTGTGAAGAACAAACATGTGGAATTCTT CCGCAACCTCTACCTGACCTTCTTGGAGTATGATGGCAACCTCCTGCGGCGGGAGCTGTTTGGCTGCCCC AGTGAGACTGATGTCAACAATGAAAATCTTCAGAAGCAGCTGTCTGAGCTGGATGAGGATGACTTGTGCT ATGAGTTCCGGCGAGAGCGCTTCACTGTCCACAGAACCCACCTGTACTTCCTGCACTACGAGTTTGAGCC TTCTGCTGACAACACAGACGTTACCCTTGTTGCTCAGCTCTCCATGGACAGACTACAGATGCTGGAGGCC ATCTGTAAACACTGGGAGGGACCCATCAGCCTGGCCCTCTACCTGTCAGATGCAGAAGCCCAGCAGTTCC TCCGCTATGCCCAGGGATCCGAGGTGCTCATGAGCCGGCAGAACGTGGGCTACCACATCGTGTACAAGGA GGGTCAGTTCTATCCTGTCAATCTTCTGCGAAACGTGGCTATGAAGCACATCAGCACACCCTACATGTTC TTATCTGACATTGACTTCCTGCCTATGTATGGGCTCTATGAGTACCTCAGGAAATCTGTCATCCAGCTTG ACCTTGCCAATACCAAGAAAGCAATGATTGTCCCAGCATTTGAAACGCTGCGCTACAGACTCTCTTTCCC CAAGTCAAAAGCAGAGCTGCTGTCAATGTTGGACATGGGGACCCTATTCACATTCAGGTATCACGTATGG ACTAAAGGCCATGCACCCACAAACTTTGCCAAGTGGCGGACTGCCACCACACCTTACCAGGTTGAGTGGG AGGCTGATTTTGAGCCATATGTTGTTGTGAGACGAGACTGCCCTGAGTATGATCGGAGATTTGTGGGCTT TGGCTGGAACAAAGTGGCTCACATCATGGAATTGGATGCACAGGAGTATGAGTTCACCGTGTTGCCCAAT GCCTACATGATCCACATGCCCCATGCCCCTAGTTTCGACATCACTAAGTTCCGTTCCAACAAGCAATACC GCATCTGTCTCAAAACCCTGAAGGAAGAGTTTCAACAAGACATGTCCCGACGATATGGCTTTGCTGCCTT GAAATATCTCACAGCTGAGAACAACAGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_010687 |
| Insert Size | 2271 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_010687.2, NP_034817.1 |
| RefSeq Size | 4671 bp |
| RefSeq ORF | 2271 bp |
| Locus ID | 16795 |
| UniProt ID | Q9Z1M7 |
| Gene Summary | Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) (PubMed:23125099, PubMed:23135544). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity and plays a key role in skeletal muscle function and regeneration (PubMed:15184894, PubMed:24132234). LARGE elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure initiated by B3GNT1/B4GAT1 by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210487 | Large (tGFP-tagged) - Mouse like-glycosyltransferase (Large) |
CNY 6,370.00 |
|
| MR210487 | Large (Myc-DDK-tagged) - Mouse like-glycosyltransferase (Large) |
CNY 5,536.00 |
|
| MR210487L3 | Lenti ORF clone of Large (Myc-DDK-tagged) - Mouse like-glycosyltransferase (Large) |
CNY 7,700.00 |
|
| MR210487L4 | Lenti ORF clone of Large (mGFP-tagged) - Mouse like-glycosyltransferase (Large) |
CNY 7,700.00 |
