United States
Germany
Japan
United Kingdom
China
Pik3r2 (NM_008841) Mouse Tagged ORF Clone
CAT＃: MG210283
- TrueORF®
Pik3r2 (tGFP-tagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta) (Pik3r2)
ORF Plasmid: DDK
"NM_008841" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 7,030.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | p85beta |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210283 representing NM_008841
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGAGCCGAGGGCTTCCAGTACAGGGCTGTGTACCCATTCCGCCGGGAGCGGCCTGAAGACCTGG AGCTGCTCCCTGGGGACCTCCTGGTGGTGAGCCGGGTGGCCCTACAGGCACTTGGTGTGGCTGATGGAGG AGAGCGCTGCCCACACAATGTGGGCTGGATGCCTGGCTTCAACGAGCGCACCCGACAGCGAGGGGACTTC CCCGGGACATACGTGGAGTTCCTAGGACCCGTGGCTCTGGCTCGACCAGGCCCTCGCCCACGGGGGCCCC GTCCGTTGCCCGCCAGGCCCTTGGATGGATCTTCTGAGTCAGGCCACATACTCCCAGACCTGGCAGAGCA GTTCTCCCCACCTGACCCTGCTCCCCCGATTCTGGTGAAGCTGGTGGAAGCCATTGAGCAAGCAGAGCTG GACAGTGAATGCTACAGTAAGCCGGAGCTGCCCGCAACACGGACAGACTGGTCCCTGAGTGACTTGGAGC AGTGGGACCGCACCGCCTTGTATGATGCTGTTAAGGGCTTCCTGCTGGCGTTGCCTGCAGCTGTGGTGAC CCCTGAAGCTGCAGCAGAGGCGTACCGGGCACTTCGAGAGGTTGCAGGCCCCGTGGGGCTGGTGCTGGAA CCCCCAACACTGCCGCTGCACCAGGCTCTCACACTGCGTTTCCTGCTGCAACACCTGGGTCGTGTGGCCC GCAGAGCACCCTCGCCAGATACAGCTGTCCATGCACTGGCCAGTGCCTTCGGGCCGCTACTGCTGCGCAT ACCTCCGTCAGGGGGCGAGGGTGATGGGAGTGAGCCTGTACCCGACTTCCCTGTGCTGCTGCTAGAGAGG CTGGTGCAGGAGCATGTGGAGGAGCAAGACGCTGCCCCCCCAGCGCTACCACCTAAGCCCTCTAAGGCAA AGCCGGCACCCACAGCTCTGGCCAATGGAGGGAGCCCGCCCTCGCTTCAGGATGCAGAGTGGTACTGGGG GGACATCTCCAGGGAAGAGGTGAATGAGAGACTCCGGGACACACCTGATGGTACCTTCTTAGTCAGAGAT GCATCCAGCAAGATCCAAGGAGAGTACACGCTCACCCTCAGGAAAGGCGGGAACAACAAGTTGATCAAAG TCTTCCACCGGGATGGTCACTATGGCTTCTCAGAGCCCCTTACCTTCTGCTCCGTGGTGGAACTCATCTC CCACTACCGCCACGAATCACTGGCCCAGTACAACGCCAAGCTGGACACACGCCTTCTCTACCCTGTGTCC AAGTACCAACAAGACCAGGTGGTGAAGGAGGACAGCATAGAGGCTGTGGGCGCCCAGCTCAAGGTCTACC ACCAGCAGTACCAGGACAAGAGCCGCGAATATGACCAGCTGTATGAAGAATACACACGGACCTCCCAGGA GCTGCAGATGAAGCGCACAGCCATAGAGGCCTTCAACGAGACCATCAAGATCTTCGAAGAGCAGGGCCAG ACACAGGAGAAATGCAGCAAGGAGTATTTGGAGCGCTTCCGGCGAGAGGGAAATGAGAAGGAGATGCAGA GGATCCTGCTGAACTCCGAGCGACTCAAGTCTCGCATCGCGGAGATACACGAAAGCCGCACGAAGTTGGA GCAGGATCTGCGGGCGCAGGCCTCCGACAACCGTGAGATCGACAAGCGCATGAACAGCCTCAAACCTGAC CTCATGCAGCTGCGCAAGATCAGGGACCAGTACCTCGTGTGGCTCACCCAGAAAGGTGCCCGACAGAGGA AGATCAACGAATGGCTGGGAATCAAGAACGAGACTGAGGACCAGTATTCACTGATGGAGGATGAGGACGC CCTCCCCCACCACGAGGAGCGCACGTGGTACGTGGGCAAGATCAACCGCACACAGGCGGAGGAGATGCTG AGTGGCAAACGAGACGGGACCTTCCTCATCCGGGAGAGCAGCCAGCGGGGCTGTTACGCATGCTCCGTGG TGGTGGACGGCGACACGAAGCACTGTGTCATCTACCGCACAGCCACCGGCTTCGGCTTCGCAGAGCCCTA TAACCTGTACGGGTCCCTGAAGGAGCTGGTGCTGCACTACCAGCACGCATCACTCGTGCAGCACAATGAC GCACTTACCGTCACCCTCGCACACCCTGTGCGTGCCCCCGGGCCTGGCCCACCGTCTGCAGCACGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210283 representing NM_008841
Red=Cloning site Green=Tags(s) MAGAEGFQYRAVYPFRRERPEDLELLPGDLLVVSRVALQALGVADGGERCPHNVGWMPGFNERTRQRGDF PGTYVEFLGPVALARPGPRPRGPRPLPARPLDGSSESGHILPDLAEQFSPPDPAPPILVKLVEAIEQAEL DSECYSKPELPATRTDWSLSDLEQWDRTALYDAVKGFLLALPAAVVTPEAAAEAYRALREVAGPVGLVLE PPTLPLHQALTLRFLLQHLGRVARRAPSPDTAVHALASAFGPLLLRIPPSGGEGDGSEPVPDFPVLLLER LVQEHVEEQDAAPPALPPKPSKAKPAPTALANGGSPPSLQDAEWYWGDISREEVNERLRDTPDGTFLVRD ASSKIQGEYTLTLRKGGNNKLIKVFHRDGHYGFSEPLTFCSVVELISHYRHESLAQYNAKLDTRLLYPVS KYQQDQVVKEDSIEAVGAQLKVYHQQYQDKSREYDQLYEEYTRTSQELQMKRTAIEAFNETIKIFEEQGQ TQEKCSKEYLERFRREGNEKEMQRILLNSERLKSRIAEIHESRTKLEQDLRAQASDNREIDKRMNSLKPD LMQLRKIRDQYLVWLTQKGARQRKINEWLGIKNETEDQYSLMEDEDALPHHEERTWYVGKINRTQAEEML SGKRDGTFLIRESSQRGCYACSVVVDGDTKHCVIYRTATGFGFAEPYNLYGSLKELVLHYQHASLVQHND ALTVTLAHPVRAPGPGPPSAAR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008841 |
| ORF Size | 2166 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008841.3 |
| RefSeq Size | 3159 bp |
| RefSeq ORF | 2169 bp |
| Locus ID | 18709 |
| UniProt ID | O08908 |
| Gene Summary | Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (By similarity). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348926).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205622 | Pik3r2 (untagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta) (Pik3r2), (10ug) |
CNY 5,296.00 |
|
| MR210283 | Pik3r2 (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta) (Pik3r2) |
CNY 5,288.00 |
|
| MR210283L3 | Lenti ORF clone of Pik3r2 (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta) (Pik3r2) |
CNY 8,270.00 |
|
| MR210283L4 | Lenti ORF clone of Pik3r2 (mGFP-tagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta) (Pik3r2) |
CNY 8,270.00 |
