United States
Germany
Japan
United Kingdom
China
Topbp1 (NM_176979) Mouse Untagged Clone
CAT＃: MC224628
Topbp1 (untagged) - Mouse topoisomerase (DNA) II binding protein 1 (Topbp1), (10ug)
CNY 19,380.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1110031N14Rik; 2810429C13Rik; AI256758; D430026L04Rik; mKIAA0259 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224628 representing NM_176979
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGAAATGACCAAGAGCCGTTTCTTGTGAAGTTTTTAAAGTCTTCGGACAATTCAGAATGCTTTT TTAAAGCTCTTGAGTCAATAAAGGAATTACAATCAGAAGATTATCTTCAGATTATTACAGATGAGGAAGC ATTGAAGATAAGAGAGAATGACAAATCGCTTTATATCTGTGACCGTTTCAGTGGCACTGTTTTTGATCAC CTTAAACAGCTTGGCTGCAGGATTGTTGGTCCTCAAGTGGTCACATTTTGTATGCGCCACCAGCAATGTG TCCCAAGAGCTGAGCATCCAGTCTACAACATGATAATGTCTGACGTAACTGTATCCTGTACAAGCCTGGA TAAAGACAAACGGGAAGAAGTTCATAAATACGTACAAATGATGGGTGGACGAGTATACAGAGACCTTAAT GTATCAGTCACTCACCTTATTGCAGGAGAAGTTGGGAGCAAAAAGTACTTAGTTGCTGCTAATCTGAAGA AGCCTATTTTGCTACCTTCTTGGATAAAAACCCTTTGGGAGAAGTCACAAGAAAAAAAAATAACCAAGTA CACTGATGTAAACATGGAAGACTTTAAGTGTCCTATTTTTCTTGGTTGCATTATCTGTGTGACTGGCTTA AATGGAATACACAGAAAGACAGTCCAGCAACTCACGGCTAAGCACGGAGGCCAGTACATGGGACAGTTGA AGATGAATGAGTGTACACACCTCATTGTGCAAGAACCAAAAGGACAAAAATACGAATGTGCCAGGAGATG GAATGTGCACTGTGTGACCCTGCAATGGTTTCATGACAGTATTGAGAAAGGCTTTTGTCAGGATGAATCT ATATACAAGGCTGAAACCAGAGTAGAAGCAAAGATGGTGCCTGATACTTCAACACCCACTGCTCAGAGCA ATGCTGAAAGTCACACGCTCGCAGATGTCAGCCATATATCCAACATAAATGGCAGCTGTGTAAATGAAAC AATGTTTGGTTCAACTACTAGCAAACTTGAATGTTCCCTTGAAAACCTAGAAAATCTGGACATCAGTATG TTTCAAGCTCCTGAAGACTTATTAGATGGCTGTCGGATATATCTTTGTGGTTTTAGTGGCAGAAAGCTGG ATAAGCTGAGAAGGCTTATTAACAGTGGAGGTGGAGTTCGATTTAATCAACTAAATGAAGATGTAACTCA TGTAATTGTGGGAGATTATGATGACGACGTGAGGCAGTTCTGGAGCAAATCATCTCACAGGCCTCACGTA GTGGGAGCAAAGTGGTTGCTGGAGTGTTTTACTAAAGGATATATACTTCCTGAAGAGTCCTATATCCACA CTAACTACCAGCCAGCTGGAATTGCAGTTTCCGATCAGCCTGGAAACCAGACAGCTGTCTTAGACAAGAG CGGCTCATTCTCCAAGAGCGCCCTTGTTCCTGCTGAGAGGCTTCAGCAGGCCGACGAGGATCTGCTTGCT CAGTATGGAAATGACGACTCCACGATGGTTGAAGCTAAGCTATCTGAAGCTTTAGAGCCTGAAGTCGGGC CTTGCCCTGGTTCTGCTCACCGTGAGCCCTGTGATGATTCCACTCACATTTCTGTGCAAGAGGAAAACAA GTCGTCTGTCAGTCATTGCATCCTTGATGACTCTACAGTTCGTGAAGAAGGCTTATTTAGCCAGAAGAGT TTCCTTGTTTTGGGTTTTAGTGTTGAAAATAAATGTAATATTGTAGACATCATAAGAGAGCATGCTGGCA AGATTGTGTCCCTTCCCAGCAGAATTGTTGCTGACTATGCTGTGGTTCCACTGCTGGGATGTGAGGTAGA CGTGACGGTGGGAGAAGTTGTTACAAACACGTGGCTGGTTACGTGTATAGACAACCAGACATTAGTTGAT CCCAAGTCGAATCCCCTCTTCACACCAGTCTCAGTAATGTCGGGAGTGACTCCTTTAGAGGACTGTGTGA TTTCCTTCAGTCAGTGTGTGGGAGCAGAAAGAGATTCCTTGGTATTCTTAGCAAATCACCTTGGAGCAAG TGTTCAAGAATTCTTTGTTCGCAAAGCCAATGCAAAGAAAGGCATGCTCGCCAGCACACATCTTATAGTG AAGGAACCGACTGGTTCCAAATACGAAGCTGCAAAGAAATGGAGTTTGCCGGCAGTTAACATTTCATGGC TCTTAGAAACTGCGAGAATCGGGAAGAGAGCAGATGAAAACCATTTTCTGGTTGACAACGCACCTAAACA AGAACAAGTTTTAGAAACTAAGATACCAAATGGAGTGAGTTCAAATCCAGATTTACCAGCACATCCTGAC GCACACTTGGAAATTCACAGAAAAAAGGCGGTTACACCTTTGGACATGAATCGCTTTCAGAGTAGAGCCT TCCGTGCTGTCATTTCACAGCAGAGGGGGCAGGACCCCACCTTTCCACCAGTACGGCAGCCCCTTACTAA GGAGCCCTCTTTACACCTGGACACACCATCAAAGTTTCTGTCCAAGGACAAGCTTTTCAAGCCATCCTTT GATGTGACGGATGCACTTGCAGCCCTGGAAACCCCAAATGCTGCCAGCCAGAAGCGGAAACTAAGCTCAC CACTCTCTGAAGTCATCGTCAGAAACCTGACAGTTGCTTTGGCAAATAGTTCCCGAAACACTGACAGTCA TTCTGCCAGCCCTCAGCTGAAGGGTGCCCATTTGGAGGAGGAAGAGACTCGGAAACCACTTGACAGCGTT GTGGTGTGTGTGAGTAAGAAGCTCAGTAAGAAGCAGAGTGAGCTCAATGGGGTCGCAGCATCACTGGGAG CAGAGTACAGGTGGAGTTTCGATGAGACTGTTACTCATTTCATTTACCAGGGTCGGGCAAATGACAGTAA TCGAGAATATAAATCTGCAAAGGAAAGAGGGGTGCACATCGTGTCCGAGCATTGGCTATTAGAGTGTGCT CAAGAGTATAAGCATCTTCCTGAATCTCTTTATCCACACACTTACAATCCCAAAATGAGTTTGGACATCA ACACAGTGCAAGATGGGCGGCTCTGTAACAGCCGGGCACCCTTGGCAGTTTCTGCATCTAAGGATGATGG GCCAGATCATCTGTCTGTAGAAGGAAATGAGACAAACACTATGGGCACAAATGACAAAGAGTCACCTCTG TTGAATGGGAGTGGAAGAGATGACTGTAAAGGAGCTCTGACCCAGGCCTTGGAGATGAGAGAGAACTTCC AGAAGCAGCTGCAAGAGATCATGTCAGCCACCTGCATTGTGAAGACGCCTGCCCAGAAGACTTGCATGTC AAGAAGCAGCTGTAACAGCGCCTCTTCAACTCCTGACAGCGCTCGCTCTGTGCGGAGCGGACGAAGCCGA GTCCTGGAGGCACTCAGGCAGTCTCGGCAGGCAGTGCCTGATGTGAACACGGAGCCGTCCCAGAACGAGC AGATCATTTGGGACGACCCGACAGCAAGGGAAGAGAGAGCAAGGCTTGCCAGCAACTTACAGTGGCCCAG CGACCCCACACAGCATTCTGAGCTTCAGGTTGAGATAAAAATGCCGGATGACTCTCCCTCTCGAAAGCCT GTGTACCATTCAGAAATTGCTGAGCAGGCCTCGTGTGTTACTCAAGCCCCAGGGCACCCAGGCTCTGAAG AACCAGAGCCTCCAGTAGCAGAAAGGCCTTTGATCCCCGAGCCGCAAGCCCCCGCTGTAGCTTCCCCCCT GGCTAAGCCGCCTGTGGCCCCACAACCTGCAGACAAGATTGAAACACAGGAGGAGACGCACAGAAAAGTT AAAAAACAGTATGTGTTCCAGATGTCGTCTCTGAATTCTCAAGAGCGGATTGATTACTGTCGCCTGATTA AAGACTTAGGTGGGTCGGTGATCGAGAAGCAGTGCTCTGACCCCAGCTGCACACACATGGTTGTGGGCTA CCCACTGCGCAATGAGAAGTACTTAGCTTCTATGGCAGCTGGTAAATGGGTGCTTCATCGCTCCTACCTA GACGCTTGCAAGACAGCTGGGCGCTTTGTGCAGGAAGAAGACTACGAGTGGGGAAGTAGCTCCATACTTG ATGCTCTCCCTGACGTCACTGAGCACCAGCAGAAGCTCGCACTGGCGGCAATGAGATGGAGGAAAAGAAT CCAGCAAAGTCAAGAATCCGGCATTGTTGAGGGAGCATTTAGTGGCTGGAAAGCTATTTTACGTGTTGAT CGGCCCCGAGAAGCTGGGTTCAAACGCCTTCTTCAGGCAGGAGGAGCAAAGGTGTTATCTGGTCATCCAG AACCTTTACTTAAAGATGCTACGCATCTCTTTTGTGACTTCAATAAACTGAAACCTGATGACTGCAGAGT TTTCATCGCAGAGGCTACTGCCCAGAACATGGTCTGCTTGAAAACAGAATATATCGCTGATTATCTCATG CTGGAATCCCCTCCTTGTGCAGACAATTACCGTGTATCCGAAGCTGCTTTATTCCATAATAAGAAAGGTG GGCCTGGATTACCACAGAAGAGGAAAACTCCTGCAGAGAATGTAGTCAAACGGCCTCGAGTACACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_176979 |
| Insert Size | 4548 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_176979.5, NP_795953.2 |
| RefSeq Size | 5112 bp |
| RefSeq ORF | 4548 bp |
| Locus ID | 235559 |
| UniProt ID | Q6ZQF0 |
| Gene Summary | Required for DNA replication (By similarity). Plays a role in the rescue of stalled replication forks and checkpoint control (PubMed:14718568). Binds double-stranded DNA breaks and nicks as well as single-stranded DNA (By similarity). Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage (By similarity). Induces a large increase in the kinase activity of ATR (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG212017 | Topbp1 (tGFP-tagged) - Mouse topoisomerase (DNA) II beta binding protein (Topbp1) |
CNY 13,300.00 |
|
| MR212017 | Topbp1 (Myc-DDK-tagged) - Mouse topoisomerase (DNA) II binding protein 1 (Topbp1) |
CNY 10,368.00 |
|
| MR212017L3 | Lenti ORF clone of Topbp1 (Myc-DDK-tagged) - Mouse topoisomerase (DNA) II binding protein 1 (Topbp1) |
CNY 13,970.00 |
|
| MR212017L4 | Lenti ORF clone of Topbp1 (mGFP-tagged) - Mouse topoisomerase (DNA) II binding protein 1 (Topbp1) |
CNY 13,970.00 |
