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Topbp1 (NM_176979) Mouse Tagged ORF Clone
CAT＃: MG212017
- TrueORF®
Topbp1 (tGFP-tagged) - Mouse topoisomerase (DNA) II beta binding protein (Topbp1)
ORF Plasmid: DDK
"NM_176979" in other vectors (4)
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CNY 13,300.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1110031N14Rik; 2810429C13Rik; AI256758; D430026L04Rik; mKIAA0259 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG212017 representing NM_176979
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGAAATGACCAAGAGCCGTTTCTTGTGAAGTTTTTAAAGTCTTCGGACAATTCAGAATGCTTTT TTAAAGCTCTTGAGTCAATAAAGGAATTACAATCAGAAGATTATCTTCAGATTATTACAGATGAGGAAGC ATTGAAGATAAGAGAGAATGACAAATCGCTTTATATCTGTGACCGTTTCAGTGGCACTGTTTTTGATCAC CTTAAACAGCTTGGCTGCAGGATTGTTGGTCCTCAAGTGGTCACATTTTGTATGCGCCACCAGCAATGTG TCCCAAGAGCTGAGCATCCAGTCTACAACATGATAATGTCTGACGTAACTGTATCCTGTACAAGCCTGGA TAAAGACAAACGGGAAGAAGTTCATAAATACGTACAAATGATGGGTGGACGAGTATACAGAGACCTTAAT GTATCAGTCACTCACCTTATTGCAGGAGAAGTTGGGAGCAAAAAGTACTTAGTTGCTGCTAATCTGAAGA AGCCTATTTTGCTACCTTCTTGGATAAAAACCCTTTGGGAGAAGTCACAAGAAAAAAAAATAACCAAGTA CACTGATGTAAACATGGAAGACTTTAAGTGTCCTATTTTTCTTGGTTGCATTATCTGTGTGACTGGCTTA AATGGAATACACAGAAAGACAGTCCAGCAACTCACGGCTAAGCACGGAGGCCAGTACATGGGACAGTTGA AGATGAATGAGTGTACACACCTCATTGTGCAAGAACCAAAAGGACAAAAATACGAATGTGCCAGGAGATG GAATGTGCACTGTGTGACCCTGCAATGGTTTCATGACAGTATTGAGAAAGGCTTTTGTCAGGATGAATCT ATATACAAGGCTGAAACCAGAGTAGAAGCAAAGATGGTGCCTGATACTTCAACACCCACTGCTCAGAGCA ATGCTGAAAGTCACACGCTCGCAGATGTCAGCCATATATCCAACATAAATGGCAGCTGTGTAAATGAAAC AATGTTTGGTTCAACTACTAGTAAACTTGAATGTTCCCTTGAAAACCTAGAAAATCTGGACATCAGTATG TTTCAAGCTCCTGAAGACTTATTAGATGGCTGTCGGATATATCTTTGTGGTTTTAGTGGCAGAAAGCTGG ATAAGCTGAGAAGGCTTATTAACAGTGGAGGTGGAGTTCGATTTAATCAACTAAATGAAGATGTAACTCA TGTAATTGTGGGAGATTATGATGACGACGTGAGGCAGTTCTGGAGCAAATCATCTCACAGGCCTCACGTA GTGGGAGCAAAGTGGTTGCTGGAGTGTTTTACTAAAGGATATATACTTCCTGAAGAGTCCTATATCCACA CTAACTACCAGCCAGCTGGAATTGCAGTTTCCGATCAGCCTGGAAACCAGACAGCTGTCTTAGACAAGAG CGGCTCATTCTCCAAGAGCGCCCTTGTTCCTGCTGAGAGGCTTCAGCAGGCCGACGAGGATCTGCTTGCT CAGTATGGAAATGACGACTCCACGATGGTTGAAGCTAAGCTATCTGAAGCTTTAGAGCCTGAAGTCGGGC CTTGCCCTGGTTCTGCTCACCGTGAGCCCTGTGATGATTCCACTCACATTTCTGTGCAAGAGGAAAACAA GTCGTCTGTCAGTCATTGCATCCTTGATGACTCTACAGTTCGTGAAGAAGGCTTATTTAGCCAGAAGAGT TTCCTTGTTTTGGGTTTTAGTGTTGAAAATAAATGTAATATTGTAGACATCATAAGAGAGCATGCTGGCA AGATTGTGTCCCTTCCCAGCAGAATTGTTGCTGACTATGCTGTGGTTCCACTGCTGGGATGTGAGGTAGA CGTGACGGTGGGAGAAGTTGTTACAAACACGTGGCTGGTTACGTGTATAGACAACCAGACATTAGTTGAT CCCAAGTCGAATCCCCTCTTCACACCAGTCTCAGTAATGTCGGGAGTGACTCCTTTAGAGGACTGTGTGA TTTCCTTCAGTCAGTGTGTGGGAGCAGAAAGAGATTCCTTGGTATTCTTAGCAAATCACCTTGGAGCAAG TGTTCAAGAATTCTTTGTTCGCAAAGCCAATGCAAAGAAAGGCATGCTCGCCAGCACACATCTTATAGTG AAGGAACCGACTGGTTCCAAATACGAAGCTGCAAAGAAATGGAGTTTGCCGGCAGTTAACATTTCATGGC TCTTAGAAACTGCGAGAATCGGGAAGAGAGCAGATGAAAACCATTTTCTGGTTGACAACGCACCTAAACA AGAACAAGTTTTAGAAACTAAGATACCAAATGGAGTGAGTTCAAATCCAGATTTACCAGCACATCCTGAC GCACACTTGGAAATTCACAGAAAAAAGGCGGTTACACCTTTGGACATGAATCGCTTTCAGAGTAGAGCCT TCCGTGCTGTCATTTCACAGCAGAGGGGGCAGGACCCCACCTTTCCACCAGTACGGCAGCCCCTTACTAA GGAGCCCTCTTTACACCTGGACACACCATCAAAGTTTCTGTCCAAGGACAAGCTTTTCAAGCCATCCTTT GATGTGACGGATGCACTTGCAGCCCTGGAAACCCCAAATGCTGCCAGCCAGAAGCGGAAACTAAGCTCAC CACTCTCTGAAGTCATCGTCAGAAACCTGACAGTTGCTTTGGCAAATAGTTCCCGAAACACTGACAGTCA TTCTGCCAGCCCTCAGCTGAAGGGTGCCCATTTGGAGGAGGAAGAGACTCGGAAACCACTTGACAGCGTT GTGGTGTGTGTGAGTAAGAAGCTCAGTAAGAAGCAGAGTGAGCTCAATGGGGTCGCAGCATCACTGGGAG CAGAGTACAGGTGGAGTTTCGATGAGACTGTTACTCATTTCATTTACCAGGGTCGGGCAAATGACAGTAA TCGAGAATATAAATCTGCAAAGGAAAGAGGGGTGCACATCGTGTCCGAGCATTGGCTATTAGAGTGTGCT CAAGAGTATAAGCATCTTCCTGAATCTCTTTATCCACACACTTACAATCCCAAAATGAGTTTGGACATCA ACACAGTGCAAGATGGGCGGCTCTGTAACAGCCGGGCACCCTTGGCAGTTTCTGCATCTAAGGATGATGG GCCAGATCATCTGTCTGTAGAAGGAAATGAGACAAACACTATGGGCACAAATGACAAAGAGTCACCTCTG TTGAATGGGAGTGGAAGAGATGACTGTAAAGGAGCTCTGACCCAGGCCTTGGAGATGAGAGAGAACTTCC AGAAGCAGCTGCAAGAGATCATGTCAGCCACCTGCATTGTGAAGACGCCTGCCCAGAAGACTTGCATGTC AAGAAGCAGCTGTAACAGCGCCTCTTCAACTCCTGACAGCGCTCGCTCTGTGCGGAGCGGACGAAGCCGA GTCCTGGAGGCACTCAGGCAGTCTCGGCAGGCAGTGCCTGATGTGAACACGGAGCCGTCCCAGAACGAGC AGATCATTTGGGACGACCCGACAGCAAGGGAAGAGAGAGCAAGGCTTGCCAGCAACTTACAGTGGCCCAG CGACCCCACACAGCATTCTGAGCTTCAGGTTGAGATAAAAATGCCGGATGACTCTCCCTCTCGAAAGCCT GTGTACCATTCAGAAATTGCTGAGCAGGCCTCGTGTGTTACTCAAGCCCCAGGGCACCCAGGCTCTGAAG AACCAGAGCCTCCAGTAGCAGAAAGGCCTTTGATCCCCGAGCCGCAAGCCCCCGCTGTAGCTTCCCCCCT GGCTAAGCCGCCTGTGGCCCCACAACCTGCAGACAAGATTGAAACACAGGAGGAGACGCACAGAAAAGTT AAAAAACAGTATGTGTTCCAGATGTCGTCTCTGAATTCTCAAGAGCGGATTGATTACTGTCGCCTGATTA AAGACTTAGGTGGGTCGGTGATCGAGAAGCAGTGCTCTGACCCCAGCTGCACACACATGGTTGTGGGCTA CCCACTGCGCAATGAGAAGTACTTAGCTTCTATGGCAGCTGGTAAATGGGTGCTTCATCGCTCCTACCTA GACGCTTGCAAGACAGCTGGGCGCTTTGTGCAGGAAGAAGACTACGAGTGGGGAAGTAGCTCCATACTTG ATGCTCTCCCTGACGTCACTGAGCACCAGCAGAAGCTCGCACTGGCGGCAATGAGATGGAGGAAAAGAAT CCAGCAAAGTCAAGAATCCGGCATTGTTGAGGGAGCATTTAGTGGCTGGAAAGCTATTTTACGTGTTGAT CGGCCCCGAGAAGCTGGGTTCAAACGCCTTCTTCAGGCAGGAGGAGCAAAGGTGTTATCTGGTCATCCAG AACCTTTACTTAAAGATGCTACGCATCTCTTTTGTGACTTCAATAAACTGAAACCTGATGACTGCAGAGT TTTCATCGCAGAGGCTACTGCCCAGAACATGGTCTGCTTGAAAACAGAATATATCGCTGATTATCTCATG CTGGAATCCCCTCCTTGTGCAGACAATTACCGTGTATCCGAAGCTGCTTTATTCCATAATAAGAAAGGTG GGCCTGGATTACCACAGAAGAGGAAAACTCCTGCAGAGAATGTAGTCAAACGGCCTCGAGTACAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG212017 representing NM_176979
Red=Cloning site Green=Tags(s) MSRNDQEPFLVKFLKSSDNSECFFKALESIKELQSEDYLQIITDEEALKIRENDKSLYICDRFSGTVFDH LKQLGCRIVGPQVVTFCMRHQQCVPRAEHPVYNMIMSDVTVSCTSLDKDKREEVHKYVQMMGGRVYRDLN VSVTHLIAGEVGSKKYLVAANLKKPILLPSWIKTLWEKSQEKKITKYTDVNMEDFKCPIFLGCIICVTGL NGIHRKTVQQLTAKHGGQYMGQLKMNECTHLIVQEPKGQKYECARRWNVHCVTLQWFHDSIEKGFCQDES IYKAETRVEAKMVPDTSTPTAQSNAESHTLADVSHISNINGSCVNETMFGSTTSKLECSLENLENLDISM FQAPEDLLDGCRIYLCGFSGRKLDKLRRLINSGGGVRFNQLNEDVTHVIVGDYDDDVRQFWSKSSHRPHV VGAKWLLECFTKGYILPEESYIHTNYQPAGIAVSDQPGNQTAVLDKSGSFSKSALVPAERLQQADEDLLA QYGNDDSTMVEAKLSEALEPEVGPCPGSAHREPCDDSTHISVQEENKSSVSHCILDDSTVREEGLFSQKS FLVLGFSVENKCNIVDIIREHAGKIVSLPSRIVADYAVVPLLGCEVDVTVGEVVTNTWLVTCIDNQTLVD PKSNPLFTPVSVMSGVTPLEDCVISFSQCVGAERDSLVFLANHLGASVQEFFVRKANAKKGMLASTHLIV KEPTGSKYEAAKKWSLPAVNISWLLETARIGKRADENHFLVDNAPKQEQVLETKIPNGVSSNPDLPAHPD AHLEIHRKKAVTPLDMNRFQSRAFRAVISQQRGQDPTFPPVRQPLTKEPSLHLDTPSKFLSKDKLFKPSF DVTDALAALETPNAASQKRKLSSPLSEVIVRNLTVALANSSRNTDSHSASPQLKGAHLEEEETRKPLDSV VVCVSKKLSKKQSELNGVAASLGAEYRWSFDETVTHFIYQGRANDSNREYKSAKERGVHIVSEHWLLECA QEYKHLPESLYPHTYNPKMSLDINTVQDGRLCNSRAPLAVSASKDDGPDHLSVEGNETNTMGTNDKESPL LNGSGRDDCKGALTQALEMRENFQKQLQEIMSATCIVKTPAQKTCMSRSSCNSASSTPDSARSVRSGRSR VLEALRQSRQAVPDVNTEPSQNEQIIWDDPTAREERARLASNLQWPSDPTQHSELQVEIKMPDDSPSRKP VYHSEIAEQASCVTQAPGHPGSEEPEPPVAERPLIPEPQAPAVASPLAKPPVAPQPADKIETQEETHRKV KKQYVFQMSSLNSQERIDYCRLIKDLGGSVIEKQCSDPSCTHMVVGYPLRNEKYLASMAAGKWVLHRSYL DACKTAGRFVQEEDYEWGSSSILDALPDVTEHQQKLALAAMRWRKRIQQSQESGIVEGAFSGWKAILRVD RPREAGFKRLLQAGGAKVLSGHPEPLLKDATHLFCDFNKLKPDDCRVFIAEATAQNMVCLKTEYIADYLM LESPPCADNYRVSEAALFHNKKGGPGLPQKRKTPAENVVKRPRVH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_176979 |
| ORF Size | 4545 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_176979.4 |
| RefSeq Size | 5112 bp |
| RefSeq ORF | 4548 bp |
| Locus ID | 235559 |
| UniProt ID | Q6ZQF0 |
| Gene Summary | Required for DNA replication (By similarity). Plays a role in the rescue of stalled replication forks and checkpoint control (PubMed:14718568). Binds double-stranded DNA breaks and nicks as well as single-stranded DNA (By similarity). Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage (By similarity). Induces a large increase in the kinase activity of ATR (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224628 | Topbp1 (untagged) - Mouse topoisomerase (DNA) II binding protein 1 (Topbp1), (10ug) |
CNY 19,380.00 |
|
| MR212017 | Topbp1 (Myc-DDK-tagged) - Mouse topoisomerase (DNA) II binding protein 1 (Topbp1) |
CNY 10,368.00 |
|
| MR212017L3 | Lenti ORF clone of Topbp1 (Myc-DDK-tagged) - Mouse topoisomerase (DNA) II binding protein 1 (Topbp1) |
CNY 13,970.00 |
|
| MR212017L4 | Lenti ORF clone of Topbp1 (mGFP-tagged) - Mouse topoisomerase (DNA) II binding protein 1 (Topbp1) |
CNY 13,970.00 |
