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Trpm5 (NM_020277) Mouse Untagged Clone
CAT＃: MC223768
Trpm5 (untagged) - Mouse transient receptor potential cation channel, subfamily M, member 5 (Trpm5), (10ug)
CNY 14,920.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 9430099A16Rik; LTrpC-5; Ltrpc5; Mtr1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223768 representing NM_020277
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAACAACCCAGAGCTCCTGCCCCGGCAGCCCCCCAGATACTGAGGATGGCTGGGAGCCCATCCTAT GCAGGGGAGAGATCAACTTCGGAGGGTCTGGGAAGAAGCGAGGCAAGTTTGTGAAGGTGCCAAGCAGTGT GGCCCCCTCCGTGCTTTTTGAACTCCTGCTCACCGAGTGGCACCTGCCAGCCCCCAACCTGGTGGTGTCC CTGGTGGGTGAGGAACGACCTTTGGCTATGAAGTCGTGGCTTCGGGATGTCCTGCGCAAGGGGCTGGTGA AAGCAGCTCAGAGCACAGGTGCCTGGATCCTGACCAGTGCCCTCCACGTGGGCCTGGCCCGCCATGTTGG ACAAGCTGTACGTGATCACTCTCTGGCTAGCACATCCACCAAGATCCGTGTAGTGGCCATCGGAATGGCC TCTCTGGATCGAATCCTTCACCGTCAACTTCTAGATGGTGTCCACCAAAAGGAGGATACTCCCATCCACT ACCCAGCAGATGAGGGCAACATTCAGGGACCCCTCTGCCCCCTGGACAGCAATCTCTCCCACTTCATCCT TGTGGAGTCAGGCGCCCTTGGGAGTGGGAACGACGGGCTGACAGAGCTGCAGCTGAGCCTGGAGAAGCAC ATCTCTCAGCAGAGGACAGGTTATGGGGGCACCAGCTGCATCCAGATACCTGTCCTTTGCCTGTTGGTCA ATGGTGACCCCAACACCCTAGAGAGGATTTCCAGGGCAGTGGAGCAGGCTGCCCCATGGCTGATCCTGGC AGGTTCTGGTGGCATTGCTGATGTACTCGCTGCCCTGGTGAGCCAGCCTCATCTCCTGGTGCCCCAGGTG GCTGAGAAGCAGTTCAGAGAGAAATTCCCCAGCGAGTGTTTCTCTTGGGAAGCCATTGTACACTGGACAG AGCTGTTACAGAACATTGCTGCACACCCCCACCTGCTCACAGTATATGACTTCGAGCAGGAGGGTTCGGA GGACCTGGACACTGTCATCCTCAAGGCACTTGTGAAAGCCTGCAAGAGCCACAGCCAAGAAGCCCAAGAC TACCTAGATGAGCTCAAGTTAGCAGTGGCCTGGGATCGCGTGGACATTGCCAAGAGTGAAATCTTCAATG GGGACGTGGAATGGAAGTCCTGTGACTTGGAAGAGGTGATGACAGATGCCCTCGTGAGCAACAAGCCTGA CTTTGTCCGCCTCTTTGTGGACAGCGGTGCTGACATGGCCGAGTTCTTGACCTATGGGCGGCTGCAGCAG CTTTACCATTCTGTGTCCCCCAAGAGCCTCCTCTTTGAACTGCTGCAGCGTAAGCATGAGGAGGGTAGGC TGACACTGGCCGGCCTGGGTGCCCAGCAGGCTCGGGAGCTGCCCATTGGTCTGCCTGCCTTCTCACTCCA CGAGGTCTCCCGCGTACTCAAAGACTTCCTGCATGACGCCTGCCGTGGCTTCTACCAGGACGGGCGCAGG ATGGAGGAGAGAGGGCCACCTAAGCGGCCCGCAGGCCAGAAGTGGCTGCCAGACCTCAGTAGGAAGAGTG AAGACCCTTGGAGGGACCTGTTCCTCTGGGCTGTGCTGCAGAATCGTTATGAGATGGCCACATACTTCTG GGCCATGGGCCGGGAGGGTGTGGCTGCTGCTCTGGCTGCCTGCAAGATCATAAAGGAAATGTCCCACCTG GAGAAAGAGGCAGAGGTGGCCCGCACCATGCGTGAGGCCAAGTATGAGCAGCTGGCCCTGGATCTTTTCT CAGAGTGCTACGGCAACAGTGAGGACCGTGCCTTTGCCCTGCTGGTGCGAAGGAACCACAGCTGGAGCAG GACCACGTGCCTGCACCTGGCCACTGAAGCTGATGCCAAGGCCTTCTTTGCCCATGACGGTGTGCAAGCA TTCCTGACCAAGATCTGGTGGGGAGACATGGCCACAGGCACACCCATCCTACGGCTTCTGGGTGCCTTCA CCTGCCCAGCCCTCATCTACACAAACCTCATCTCCTTCAGTGAGGATGCCCCGCAGAGGATGGACCTAGA AGATCTGCAGGAGCCAGACAGCTTGGATATGGAAAAGAGCTTCCTATGCAGCCGGGGTGGCCAATTGGAG AAGCTAACAGAGGCACCAAGGGCTCCAGGCGATCTAGGCCCACAAGCTGCCTTCCTGCTCACACGGTGGA GGAAGTTCTGGGGCGCTCCTGTGACTGTGTTCCTGGGGAATGTGGTCATGTACTTCGCATTCCTCTTCCT GTTCACCTATGTCCTGCTGGTGGACTTCAGGCCACCACCCCAGGGGCCGTCTGGATCCGAGGTTACCCTC TATTTCTGGGTGTTCACACTGGTGCTGGAGGAAATCCGACAGGGCTTCTTCACAGATGAGGACACGCACC TGGTGAAGAAATTCACTCTGTATGTGGAAGACAACTGGAACAAGTGTGACATGGTGGCCATCTTCCTGTT CATTGTGGGAGTCACCTGTAGAATGGTGCCCTCGGTGTTTGAGGCTGGCAGGACCGTTCTGGCCATTGAC TTCATGGTGTTCACACTTCGGCTCATCCACATCTTTGCTATTCACAAGCAGTTGGGTCCTAAGATCATCA TTGTAGAGCGAATGATGAAGGATGTCTTCTTTTTCCTCTTCTTCCTGAGCGTATGGCTTGTGGCCTATGG TGTGACCACTCAGGCCCTGCTGCATCCCCATGATGGCCGTTTGGAGTGGATTTTCCGCCGTGTGCTATAC AGGCCTTACCTGCAGATCTTTGGGCAAATCCCTCTGGATGAAATTGATGAGGCTCGTGTGAACTGTTCTC TTCACCCTCTGCTGCTGGAAAGCTCGGCTTCCTGCCCTAATCTCTATGCCAACTGGCTGGTCATTCTCCT GCTGGTTACCTTCCTGCTTGTCACTAATGTGCTGCTCATGAACCTTCTGATCGCCATGTTCAGCTACACA TTCCAGGTGGTGCAAGGCAATGCAGACATGTTCTGGAAGTTTCAACGCTACCACCTCATCGTTGAATACC ATGGAAGACCAGCTCTGGCCCCGCCCTTCATCCTGCTCAGCCACCTGAGCCTGGTGCTCAAGCAGGTCTT CAGGAAGGAAGCCCAGCATAAGCGACAACATCTGGAGAGAGACTTGCCTGACCCCTTGGACCAGAAGATC ATTACCTGGGAAACGGTTCAAAAGGAGAACTTCCTGAGTACCATGGAGAAACGGAGGAGGGACAGCGAGG GGGAGGTGCTGAGGAAAACGGCACACAGAGTGGACTTGATTGCCAAATACATCGGGGGGCTGAGAGAGCA AGAAAAGAGGATCAAGTGTCTGGAATCACAGGCCAACTACTGTATGCTCCTCTTGTCCTCTATGACGGAT ACACTGGCTCCAGGAGGCACCTACTCAAGCTCTCAGAACTGTGGTTGCAGGAGTCAGCCAGCCTCTGCTA GAGACAGGGAGTACCTAGAGTCTGGCTTGCCACCCTCTGACACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_020277 |
| Insert Size | 3477 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020277.2, NP_064673.2 |
| RefSeq Size | 4383 bp |
| RefSeq ORF | 3477 bp |
| Locus ID | 56843 |
| UniProt ID | Q9JJH7 |
| Gene Summary | Voltage-modulated Ca(2+)-activated, monovalent cation channel (VCAM) that mediates a transient membrane depolarization and plays a central role in taste transduction. Monovalent-specific, non-selective cation channel that mediates the transport of Na(+), K(+) and Cs(+) ions equally well. Activated directly by increases in intracellular Ca(2+), but is impermeable to it. Gating is voltage-dependent and displays rapid activation and deactivation kinetics upon channel stimulation even during sustained elevations in Ca(2+). Also activated by a fast intracellular Ca(2+) increase in response to inositol 1,4,5-triphosphate-producing receptor agonists. The channel is blocked by extracellular acidification. External acidification has 2 effects, a fast reversible block of the current and a slower irreversible enhancement of current inactivation. Is a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. Heat activation is due to a shift of the voltage-dependent activation curve to negative potentials. Activated by arachidonic acid in vitro. May be involved in perception of bitter, sweet and umami tastes. May also be involved in sensing semiochemicals.[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227492 | Trpm5 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily M member 5 (Trpm5), (10ug) |
CNY 9,664.00 |
|
| MR227492 | Trpm5 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 5 (Trpm5) |
CNY 8,064.00 |
|
| MR227492L3 | Lenti ORF clone of Trpm5 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 5 (Trpm5) |
CNY 10,260.00 |
|
| MR227492L4 | Lenti ORF clone of Trpm5 (mGFP-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 5 (Trpm5) |
CNY 10,260.00 |
