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Trpm5 (NM_020277) Mouse Tagged ORF Clone
CAT＃: MG227492
- TrueORF®
Trpm5 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily M member 5 (Trpm5), (10ug)
ORF Plasmid: DDK
"NM_020277" in other vectors (4)
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CNY 9,664.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9430099A16Rik; LTrpC-5; Ltrpc5; Mtr1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227492 representing NM_020277
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAACAACCCAGAGCTCCTGCCCCGGCAGCCCCCCAGATACTGAGGATGGCTGGGAGCCCATCCTAT GCAGGGGAGAGATCAACTTCGGAGGGTCTGGGAAGAAGCGAGGCAAGTTTGTGAAGGTGCCAAGCAGTGT GGCCCCCTCCGTGCTTTTTGAACTCCTGCTCACCGAGTGGCACCTGCCAGCCCCCAACCTGGTGGTGTCC CTGGTGGGTGAGGAACGACCTTTGGCTATGAAGTCGTGGCTTCGGGATGTCCTGCGCAAGGGGCTGGTGA AAGCAGCTCAGAGCACAGGTGCCTGGATCCTGACCAGTGCCCTCCACGTGGGCCTGGCCCGCCATGTTGG ACAAGCTGTACGTGATCACTCTCTGGCTAGCACATCCACCAAGATCCGTGTAGTGGCCATCGGAATGGCC TCTCTGGATCGAATCCTTCACCGTCAACTTCTAGATGGTGTCCACCAAAAGGAGGATACTCCCATCCACT ACCCAGCAGATGAGGGCAACATTCAGGGACCCCTCTGCCCCCTGGACAGCAATCTCTCCCACTTCATCCT TGTGGAGTCAGGCGCCCTTGGGAGTGGGAACGACGGGCTGACAGAGCTGCAGCTGAGCCTGGAGAAGCAC ATCTCTCAGCAGAGGACAGGTTATGGGGGCACCAGCTGCATCCAGATACCTGTCCTTTGCCTGTTGGTCA ATGGTGACCCCAACACCCTAGAGAGGATTTCCAGGGCAGTGGAGCAGGCTGCCCCATGGCTGATCCTGGC AGGTTCTGGTGGCATTGCTGATGTACTCGCTGCCCTGGTGAGCCAGCCTCATCTCCTGGTGCCCCAGGTG GCTGAGAAGCAGTTCAGAGAGAAATTCCCCAGCGAGTGTTTCTCTTGGGAAGCCATTGTACACTGGACAG AGCTGTTACAGAACATTGCTGCACACCCCCACCTGCTCACAGTATATGACTTCGAGCAGGAGGGTTCGGA GGACCTGGACACTGTCATCCTCAAGGCACTTGTGAAAGCCTGCAAGAGCCACAGCCAAGAAGCCCAAGAC TACCTAGATGAGCTCAAGTTAGCAGTGGCCTGGGATCGCGTGGACATTGCCAAGAGTGAAATCTTCAATG GGGACGTGGAATGGAAGTCCTGTGACTTGGAAGAGGTGATGACAGATGCCCTCGTGAGCAACAAGCCTGA CTTTGTCCGCCTCTTTGTGGACAGCGGTGCTGACATGGCCGAGTTCTTGACCTATGGGCGGCTGCAGCAG CTTTACCATTCTGTGTCCCCCAAGAGCCTCCTCTTTGAACTGCTGCAGCGTAAGCATGAGGAGGGTAGGC TGACACTGGCCGGCCTGGGTGCCCAGCAGGCTCGGGAGCTGCCCATTGGTCTGCCTGCCTTCTCACTCCA CGAGGTCTCCCGCGTACTCAAAGACTTCCTGCATGACGCCTGCCGTGGCTTCTACCAGGACGGGCGCAGG ATGGAGGAGAGAGGGCCACCTAAGCGGCCCGCAGGCCAGAAGTGGCTGCCAGACCTCAGTAGGAAGAGTG AAGACCCTTGGAGGGACCTGTTCCTCTGGGCTGTGCTGCAGAATCGTTATGAGATGGCCACATACTTCTG GGCCATGGGCCGGGAGGGTGTGGCTGCTGCTCTGGCTGCCTGCAAGATCATAAAGGAAATGTCCCACCTG GAGAAAGAGGCAGAGGTGGCCCGCACCATGCGTGAGGCCAAGTATGAGCAGCTGGCCCTGGATCTTTTCT CAGAGTGCTACGGCAACAGTGAGGACCGTGCCTTTGCCCTGCTGGTGCGAAGGAACCACAGCTGGAGCAG GACCACGTGCCTGCACCTGGCCACTGAAGCTGATGCCAAGGCCTTCTTTGCCCATGACGGTGTGCAAGCA TTCCTGACCAAGATCTGGTGGGGAGACATGGCCACAGGCACACCCATCCTACGGCTTCTGGGTGCCTTCA CCTGCCCAGCCCTCATCTACACAAACCTCATCTCCTTCAGTGAGGATGCCCCGCAGAGGATGGACCTAGA AGATCTGCAGGAGCCAGACAGCTTGGATATGGAAAAGAGCTTCCTATGCAGCCGGGGTGGCCAATTGGAG AAGCTAACAGAGGCACCAAGGGCTCCAGGCGATCTAGGCCCACAAGCTGCCTTCCTGCTCACACGGTGGA GGAAGTTCTGGGGCGCTCCTGTGACTGTGTTCCTGGGGAATGTGGTCATGTACTTCGCATTCCTCTTCCT GTTCACCTATGTCCTGCTGGTGGACTTCAGGCCACCACCCCAGGGGCCGTCTGGATCCGAGGTTACCCTC TATTTCTGGGTGTTCACACTGGTGCTGGAGGAAATCCGACAGGGCTTCTTCACAGATGAGGACACGCACC TGGTGAAGAAATTCACTCTGTATGTGGAAGACAACTGGAACAAGTGTGACATGGTGGCCATCTTCCTGTT CATTGTGGGAGTCACCTGTAGAATGGTGCCCTCGGTGTTTGAGGCTGGCAGGACCGTTCTGGCCATTGAC TTCATGGTGTTCACACTTCGGCTCATCCACATCTTTGCTATTCACAAGCAGTTGGGTCCTAAGATCATCA TTGTAGAGCGAATGATGAAGGATGTCTTCTTTTTCCTCTTCTTCCTGAGCGTATGGCTTGTGGCCTATGG TGTGACCACTCAGGCCCTGCTGCATCCCCATGATGGCCGTTTGGAGTGGATTTTCCGCCGTGTGCTATAC AGGCCTTACCTGCAGATCTTTGGGCAAATCCCTCTGGATGAAATTGATGAGGCTCGTGTGAACTGTTCTC TTCACCCTCTGCTGCTGGAAAGCTCGGCTTCCTGCCCTAATCTCTATGCCAACTGGCTGGTCATTCTCCT GCTGGTTACCTTCCTGCTTGTCACTAATGTGCTGCTCATGAACCTTCTGATCGCCATGTTCAGCTACACA TTCCAGGTGGTGCAAGGCAATGCAGACATGTTCTGGAAGTTTCAACGCTACCACCTCATCGTTGAATACC ATGGAAGACCAGCTCTGGCCCCGCCCTTCATCCTGCTCAGCCACCTGAGCCTGGTGCTCAAGCAGGTCTT CAGGAAGGAAGCCCAGCATAAGCGACAACATCTGGAGAGAGACTTGCCTGACCCCTTGGACCAGAAGATC ATTACCTGGGAAACGGTTCAAAAGGAGAACTTCCTGAGTACCATGGAGAAACGGAGGAGGGACAGCGAGG GGGAGGTGCTGAGGAAAACGGCACACAGAGTGGACTTGATTGCCAAATACATCGGGGGGCTGAGAGAGCA AGAAAAGAGGATCAAGTGTCTGGAATCACAGGCCAACTACTGTATGCTCCTCTTGTCCTCTATGACGGAT ACACTGGCTCCAGGAGGCACCTACTCAAGCTCTCAGAACTGTGGTTGCAGGAGTCAGCCAGCCTCTGCTA GAGACAGGGAGTACCTAGAGTCTGGCTTGCCACCCTCTGACACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227492 representing NM_020277
Red=Cloning site Green=Tags(s) MQTTQSSCPGSPPDTEDGWEPILCRGEINFGGSGKKRGKFVKVPSSVAPSVLFELLLTEWHLPAPNLVVS LVGEERPLAMKSWLRDVLRKGLVKAAQSTGAWILTSALHVGLARHVGQAVRDHSLASTSTKIRVVAIGMA SLDRILHRQLLDGVHQKEDTPIHYPADEGNIQGPLCPLDSNLSHFILVESGALGSGNDGLTELQLSLEKH ISQQRTGYGGTSCIQIPVLCLLVNGDPNTLERISRAVEQAAPWLILAGSGGIADVLAALVSQPHLLVPQV AEKQFREKFPSECFSWEAIVHWTELLQNIAAHPHLLTVYDFEQEGSEDLDTVILKALVKACKSHSQEAQD YLDELKLAVAWDRVDIAKSEIFNGDVEWKSCDLEEVMTDALVSNKPDFVRLFVDSGADMAEFLTYGRLQQ LYHSVSPKSLLFELLQRKHEEGRLTLAGLGAQQARELPIGLPAFSLHEVSRVLKDFLHDACRGFYQDGRR MEERGPPKRPAGQKWLPDLSRKSEDPWRDLFLWAVLQNRYEMATYFWAMGREGVAAALAACKIIKEMSHL EKEAEVARTMREAKYEQLALDLFSECYGNSEDRAFALLVRRNHSWSRTTCLHLATEADAKAFFAHDGVQA FLTKIWWGDMATGTPILRLLGAFTCPALIYTNLISFSEDAPQRMDLEDLQEPDSLDMEKSFLCSRGGQLE KLTEAPRAPGDLGPQAAFLLTRWRKFWGAPVTVFLGNVVMYFAFLFLFTYVLLVDFRPPPQGPSGSEVTL YFWVFTLVLEEIRQGFFTDEDTHLVKKFTLYVEDNWNKCDMVAIFLFIVGVTCRMVPSVFEAGRTVLAID FMVFTLRLIHIFAIHKQLGPKIIIVERMMKDVFFFLFFLSVWLVAYGVTTQALLHPHDGRLEWIFRRVLY RPYLQIFGQIPLDEIDEARVNCSLHPLLLESSASCPNLYANWLVILLLVTFLLVTNVLLMNLLIAMFSYT FQVVQGNADMFWKFQRYHLIVEYHGRPALAPPFILLSHLSLVLKQVFRKEAQHKRQHLERDLPDPLDQKI ITWETVQKENFLSTMEKRRRDSEGEVLRKTAHRVDLIAKYIGGLREQEKRIKCLESQANYCMLLLSSMTD TLAPGGTYSSSQNCGCRSQPASARDREYLESGLPPSDT TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020277 |
| ORF Size | 3474 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020277.2, NP_064673.2 |
| RefSeq Size | 4383 bp |
| RefSeq ORF | 3477 bp |
| Locus ID | 56843 |
| UniProt ID | Q9JJH7 |
| Gene Summary | Voltage-modulated Ca(2+)-activated, monovalent cation channel (VCAM) that mediates a transient membrane depolarization and plays a central role in taste transduction. Monovalent-specific, non-selective cation channel that mediates the transport of Na(+), K(+) and Cs(+) ions equally well. Activated directly by increases in intracellular Ca(2+), but is impermeable to it. Gating is voltage-dependent and displays rapid activation and deactivation kinetics upon channel stimulation even during sustained elevations in Ca(2+). Also activated by a fast intracellular Ca(2+) increase in response to inositol 1,4,5-triphosphate-producing receptor agonists. The channel is blocked by extracellular acidification. External acidification has 2 effects, a fast reversible block of the current and a slower irreversible enhancement of current inactivation. Is a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. Heat activation is due to a shift of the voltage-dependent activation curve to negative potentials. Activated by arachidonic acid in vitro. May be involved in perception of bitter, sweet and umami tastes. May also be involved in sensing semiochemicals.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223768 | Trpm5 (untagged) - Mouse transient receptor potential cation channel, subfamily M, member 5 (Trpm5), (10ug) |
CNY 14,920.00 |
|
| MR227492 | Trpm5 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 5 (Trpm5) |
CNY 8,064.00 |
|
| MR227492L3 | Lenti ORF clone of Trpm5 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 5 (Trpm5) |
CNY 10,260.00 |
|
| MR227492L4 | Lenti ORF clone of Trpm5 (mGFP-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 5 (Trpm5) |
CNY 10,260.00 |
