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Pink1 (NM_026880) Mouse Untagged Clone
CAT＃: MC205235
Pink1 (untagged) - Mouse PTEN induced putative kinase 1 (Pink1), nuclear gene encoding mitochondrial protein, (10ug)
CN¥ 6,488.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1190006F07Rik; AU042772; AW557854; BRPK; mFLJ00387 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC067066
CAGACGTTTGTGGCGGTGGCAGCTGGCTCCGGTCACCGCGCCACCATGGCGGTGCGACAGGCACTGGGCC GAGGCCTGCAGCTGGGTCGGGCGCTGCTGCTGCGCTTCGCGCCCAAGCCCGGCCCGCTGTTCGGCTGGGG GAAGCCCGGCCCCGCGGCGGCCTGGGGCCGCGGAGAGCGCCCAGGCCAGGTCGTAAGCCCCGGAGCACAG CCTCGGCCGGTCGGGCTCCCCCTCCCGGACCGCTACCGCTTCTTCCGCCAGTCGGTAGCTGGGCTGGCGG CGCGGATCCAGAGGCAGTTCATGGTGCGGGCCCGGGGCGGCGCAGGCCCTTGCGGCCGAGCGGTCTTCCT GGCCTTCGGGCTGGGGCTGGGGCTGATCGAGGAGAAGCAGGCGGAAGGCCGGAGGGCCGCCTCGGCCTGT CAGGAGATCCAGGCAATTTTTACACAGAAAACCAAGCGCGTGTCTGACCCACTGGACACTCGATGCTGGC AGGGCTTCCGTCTGGAGGATTATCTGATAGGGCAAGCCATTGGCAAGGGTTGCAATGCCGCTGTGTATGA AGCCACCATGCCCACGCTGCCCCAGCACCTGGAAAAGGCCAAACACCTTGGCCTTATAGGAAAGGGCCCG GATGTCGTCCTGAAGGGAGCAGACGGGGAGCAGGCTCCAGGGACTCCCACCTTTCCCTTTGCCATCAAGA TGATGTGGAATATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTAAGCAAAATGAGCCAGGAGCTGGTCCC GGCAAGCCGCGTGGCTTTGGCTGGAGAGTATGGAGCAGTTACTTACAGAAGATCCAGAGATGGTCCCAAG CAGCTTGCCCCACACCCTAACATCATCCGGGTTTTCCGCGCCTTCACCTCATCTGTGCCCCTCCTGCCGG GGGCCCTGGCTGACTATCCTGATATGCTGCCCCCACACTACTACCCAGAAGGCCTGGGCCACGGTCGCAC ACTGTTCCTCGTTATGAAGAACTACCCCTGTACCCTGCGCCAGTACCTTGAGGAGCAGACTCCCAGTTCT CGCCTGGCTACCATGATGACCTTGCAGTTGCTGGAGGGCGTGGACCATCTGGTTCAGCAGGGCATTGCCC ATCGGGATCTCAAGTCCGACAACATCCTTGTGGAGTGGGACTCAGATGGCTGTCCCTGGCTAGTGATCTC AGACTTTGGCTGCTGCCTGGCTGACCAGCATGTTGGCCTGCGGCTGCCTTTCAACAGCTCCAGTGTAGAG CGTGGTGGCAATGGCTCCCTGATGGCCCCTGAGGTGTCCACAGCCCATTCTGGCCCCAGTGCGGTAATTG ACTACAGCAAAGCCGATACCTGGGCTGTGGGGGCCATCGCCTATGAAATCTTTGGGCTTGCCAATCCCTT CTATGGCCAAGGCAGTGCCCACCTCGAGAGCCGCAGCTACCAGGAAGCTCAGCTGCCTGAGATGCCTGAG TCGGTGCCTCCAGAGGCAAGACGGCTGGTGAGGTCACTGCTCCAGCGAGAGGCCAGCAAGAGACCGTCTG CACGCTTAGCTGCAAATGTGCTGCACTTAAGCCTCTGGGGCGAGCATCTTCTAGCCCTGAAGAACCTGAA ATTGGACAAGATGATCGCCTGGCTCTTGCAGCAGTCAGCAGCCACTCTGCTGGCTGACAGGCTGAGAGAG AAGAGCTGCGTGGAGACAAAGCTGCAGATGCTGTTTCTGGCTAACCTGGAGTGTGAGGCTCTCTGCCAGG CAGCCCTCCTCCTCTCTTCCTGGAGGGCAGCCCCATGATGGCTCTGTGCTCCAGTTACTAAAAGACTAAG GGGCATCTGTGTCCTGATGTCATGACAGTATACGAAGGCTGAAGAAGGTGGCTTGGCTGGGTGGGCAGGA GTCAGGGGACAAGCTGGAGTAGAGGGAGGGGCTGGTCAGCCTTGGGCTCAGCAAATGGGAAGAACAGCCT TGAACAGCTCAGGAGGGAGAGAGGCACTACCTGTGCCCGTCCAGTTAGGTTCTTGGGCAGAATTAAGACA TCAGGCCAGACCAGGAGTTGGTAAGCTATAGAGGGCCAGACAGGGAATGAAGTAGTTGTAATGACTAACC TATCACTGAGGCAAAGGCCAAGCAAGCACTGCAGTGAGGCCAACACTGAACTTTGCTTGTCGTCAATAAG TTCTGCTCATTTTTCCCAACCACTTAGAAATGCAAAAACATAGGTTTAATTTGTAAGCAGCAACCAGCAT GGCTCATAGTCAAATTTACCAATTCCTGGGCTAAATGCATTCTTTATGTCTAACTCTTTAAAGATGCGAG CATTAAAGACAGTCTCCATGCCAAAAAAAAAAAAAAAAAA |
| Restriction Sites | AscI-NotI |
| ACCN | NM_026880 |
| Insert Size | 1743 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC067066, AAH67066 |
| RefSeq Size | 2350 bp |
| RefSeq ORF | 1743 bp |
| Locus ID | 68943 |
| UniProt ID | Q99MQ3 |
| Gene Summary | Protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins (PubMed:24652937). Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PRKN (PubMed:24784582). Targets PRKN to dysfunctional depolarized mitochondria through the phosphorylation of MFN2 (By similarity). Activates PRKN in 2 steps: (1) by mediating phosphorylation at 'Ser-65' of PRKN and (2) mediating phosphorylation of ubiquitin, converting PRKN to its fully-active form (PubMed:24784582). Required for ubiquinone reduction by mitochondrial complex I by mediating phosphorylation of complex I subunit NDUFA10 (PubMed:24652937).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209092 | Pink1 (tGFP-tagged) - Mouse PTEN induced putative kinase 1 (Pink1) |
CN¥ 8,080.00 |
|
| MR209092 | Pink1 (Myc-DDK-tagged) - Mouse PTEN induced putative kinase 1 (Pink1), nuclear gene encoding mitochondrial protein |
CN¥ 6,480.00 |
|
| MR209092L1 | Lenti ORF clone of Pink1 (Myc-DDK-tagged) - Mouse PTEN induced putative kinase 1 (Pink1), nuclear gene encoding mitochondrial protein |
CN¥ 8,880.00 |
|
| MR209092L2 | Lenti ORF clone of Pink1 (mGFP-tagged) - Mouse PTEN induced putative kinase 1 (Pink1), nuclear gene encoding mitochondrial protein |
CN¥ 8,880.00 |
|
| MR209092L3 | Lenti ORF clone of Pink1 (Myc-DDK-tagged) - Mouse PTEN induced putative kinase 1 (Pink1), nuclear gene encoding mitochondrial protein |
CN¥ 7,700.00 |
|
| MR209092L4 | Lenti ORF clone of Pink1 (mGFP-tagged) - Mouse PTEN induced putative kinase 1 (Pink1), nuclear gene encoding mitochondrial protein |
CN¥ 8,880.00 |
