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Pink1 (NM_026880) Mouse Tagged ORF Clone
CAT＃: MG209092
- TrueORF®
Pink1 (tGFP-tagged) - Mouse PTEN induced putative kinase 1 (Pink1)
ORF Plasmid: DDK
"NM_026880" in other vectors (6)
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CNY 8,080.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1190006F07Rik; AU042772; AW557854; BRPK; mFLJ00387 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209092 representing NM_026880
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTGCGACAGGCACTGGGCCGAGGCCTGCAGCTGGGTCGGGCGCTGCTGCTGCGCTTCGCGCCCA AGCCCGGCCCGCTGTTCGGCTGGGGGAAGCCCGGCCCCGCGGCGGCCTGGGGCCGCGGAGAGCGCCCAGG CCAGGTCGTAAGCCCCGGAGCACAGCCTCGGCCGGTCGGGCTCCCCCTCCCGGACCGCTACCGCTTCTTC CGCCAGTCGGTAGCTGGGCTGGCGGCGCGGATCCAGAGGCAGTTCATGGTGCGGGCCCGGGGCGGCGCAG GCCCTTGCGGCCGAGCGGTCTTCCTGGCCTTCGGGCTGGGGCTGGGGCTGATCGAGGAGAAGCAGGCGGA AGGCCGGAGGGCCGCCTCGGCCTGTCAGGAGATCCAGGCAATTTTTACACAGAAAACCAAGCGCGTGTCT GACCCACTGGACACTCGATGCTGGCAGGGCTTCCGTCTGGAGGATTATCTGATAGGGCAAGCCATTGGCA AGGGTTGCAATGCCGCTGTGTATGAAGCCACCATGCCCACGCTGCCCCAGCACCTGGAAAAGGCCAAACA CCTTGGCCTTATAGGAAAGGGCCCGGATGTCGTCCTGAAGGGAGCAGACGGGGAGCAGGCTCCAGGGACT CCCACCTTTCCCTTTGCCATCAAGATGATGTGGAATATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTAA GCAAAATGAGCCAGGAGCTGGTCCCGGCAAGCCGCGTGGCTTTGGCTGGAGAGTATGGAGCAGTTACTTA CAGAAGATCCAGAGATGGTCCCAAGCAGCTTGCCCCACACCCTAACATCATCCGGGTTTTCCGCGCCTTC ACCTCATCTGTGCCCCTCCTGCCGGGGGCCCTGGCTGACTATCCTGATATGCTGCCCCCACACTACTACC CAGAAGGCCTGGGCCACGGTCGCACACTGTTCCTCGTTATGAAGAACTACCCCTGTACCCTGCGCCAGTA CCTTGAGGAGCAGACTCCCAGTTCTCGCCTGGCTACCATGATGACCTTGCAGTTGCTGGAGGGCGTGGAC CATCTGGTTCAGCAGGGCATTGCCCATCGGGATCTCAAGTCCGACAACATCCTTGTGGAGTGGGACTCAG ATGGCTGTCCCTGGCTAGTGATCTCAGACTTTGGCTGCTGCCTGGCTGACCAGCATGTTGGCCTGCGGCT GCCTTTCAACAGCTCCAGTGTAGAGCGTGGTGGCAATGGCTCCCTGATGGCCCCTGAGGTGTCCACAGCC CATTCTGGCCCCAGTGCGGTAATTGACTACAGCAAAGCCGATACCTGGGCTGTGGGGGCCATCGCCTATG AAATCTTTGGGCTTGCCAATCCCTTCTATGGCCAAGGCAGTGCCCACCTCGAGAGCCGCAGCTACCAGGA AGCTCAGCTGCCTGAGATGCCTGAGTCGGTGCCTCCAGAGGCAAGACGGCTGGTGAGGTCACTGCTCCAG CGAGAGGCCAGCAAGAGACCGTCTGCACGCTTAGCTGCAAATGTGCTGCACTTAAGCCTCTGGGGCGAGC ATCTTCTAGCCCTGAAGAACCTGAAATTGGACAAGATGATCGCCTGGCTCTTGCAGCAGTCAGCAGCCAC TCTGCTGGCTGACAGGCTGAGAGAGAAGAGCTGCGTGGAGACAAAGCTGCAGATGCTGTTTCTGGCTAAC CTGGAGTGTGAGGCTCTCTGCCAGGCAGCCCTCCTCCTCTCTTCCTGGAGGGCAGCCCCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209092 representing NM_026880
Red=Cloning site Green=Tags(s) MAVRQALGRGLQLGRALLLRFAPKPGPLFGWGKPGPAAAWGRGERPGQVVSPGAQPRPVGLPLPDRYRFF RQSVAGLAARIQRQFMVRARGGAGPCGRAVFLAFGLGLGLIEEKQAEGRRAASACQEIQAIFTQKTKRVS DPLDTRCWQGFRLEDYLIGQAIGKGCNAAVYEATMPTLPQHLEKAKHLGLIGKGPDVVLKGADGEQAPGT PTFPFAIKMMWNISAGSSSEAILSKMSQELVPASRVALAGEYGAVTYRRSRDGPKQLAPHPNIIRVFRAF TSSVPLLPGALADYPDMLPPHYYPEGLGHGRTLFLVMKNYPCTLRQYLEEQTPSSRLATMMTLQLLEGVD HLVQQGIAHRDLKSDNILVEWDSDGCPWLVISDFGCCLADQHVGLRLPFNSSSVERGGNGSLMAPEVSTA HSGPSAVIDYSKADTWAVGAIAYEIFGLANPFYGQGSAHLESRSYQEAQLPEMPESVPPEARRLVRSLLQ REASKRPSARLAANVLHLSLWGEHLLALKNLKLDKMIAWLLQQSAATLLADRLREKSCVETKLQMLFLAN LECEALCQAALLLSSWRAAP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026880 |
| ORF Size | 1740 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026880.2, NP_081156.2 |
| RefSeq Size | 2367 bp |
| RefSeq ORF | 1743 bp |
| Locus ID | 68943 |
| UniProt ID | Q99MQ3 |
| Gene Summary | Protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins (PubMed:24652937). Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PRKN (PubMed:24784582). Targets PRKN to dysfunctional depolarized mitochondria through the phosphorylation of MFN2 (By similarity). Activates PRKN in 2 steps: (1) by mediating phosphorylation at 'Ser-65' of PRKN and (2) mediating phosphorylation of ubiquitin, converting PRKN to its fully-active form (PubMed:24784582). Required for ubiquinone reduction by mitochondrial complex I by mediating phosphorylation of complex I subunit NDUFA10 (PubMed:24652937).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205235 | Pink1 (untagged) - Mouse PTEN induced putative kinase 1 (Pink1), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 6,488.00 |
|
| MR209092 | Pink1 (Myc-DDK-tagged) - Mouse PTEN induced putative kinase 1 (Pink1), nuclear gene encoding mitochondrial protein |
CNY 6,480.00 |
|
| MR209092L1 | Lenti ORF clone of Pink1 (Myc-DDK-tagged) - Mouse PTEN induced putative kinase 1 (Pink1), nuclear gene encoding mitochondrial protein |
CNY 8,880.00 |
|
| MR209092L2 | Lenti ORF clone of Pink1 (mGFP-tagged) - Mouse PTEN induced putative kinase 1 (Pink1), nuclear gene encoding mitochondrial protein |
CNY 8,880.00 |
|
| MR209092L3 | Lenti ORF clone of Pink1 (Myc-DDK-tagged) - Mouse PTEN induced putative kinase 1 (Pink1), nuclear gene encoding mitochondrial protein |
CNY 7,700.00 |
|
| MR209092L4 | Lenti ORF clone of Pink1 (mGFP-tagged) - Mouse PTEN induced putative kinase 1 (Pink1), nuclear gene encoding mitochondrial protein |
CNY 8,880.00 |
