pAAVS1-EF1a-Luc-BSD-DNR

CAT＃: GE100073

AAVS1 donor vector with AAVS1 homologous arms, firefly luciferase under EF1a promoter, and Blasticidin selection (10 ug)

Datasheet
SDS
Vector Sequence

CN¥ 7,505.00

货期*
现货
规格
    • 10 ug

Product images

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Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
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Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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Specifications

Product Data
Function AAVS1 donor vector
Features
  • Donor vector for targeted transgene insertion into human AAVS1 site
  • Contains left and right AAVS1 homologous sequences (550 bp each side)
  • Multiple cloning sites for transgene cloning, compatible for shuttling from the TrueORF clones
  • The expression of the target gene will be under EF1a promoter, a constitutive promoter as an alternative of CMV promoter. the latter could be silenced in certain cellular environment
  • Blasticidin mammalian selection under PGK promoter
  • Targeted insertion when cotransfected with pCas-Guide-AAVS1 (SKU GE100023)
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
FAQs
SDS
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