pCas-Guide-AAVS1

CAT＃: GE100023

AAVS1 gRNA vector, validated AAVS1 targeting sequence cloned in pCas-Guide vector

Datasheet
SDS
Vector Sequence

CNY 4,560.00

货期*
现货
规格
    • 10 ug
Cited in 7 publications.

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Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
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TP790151
Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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暂停销售

Specifications

Product Data
Function All-in-One CRISPR/Cas9 vectors
Features
  • Validated CRISPR vector targeting human AAVS1 site
  • AAVS1 target sequence: GTTAATGTGGCTCTGGTTCT
  • CMV-driven Cas9 and U6-driven AAVS1 gRNA expression

Applications

  • Targeted transgene insertion via CRISPR when cotransfected with pAAVS1-puro-DNR (after transgene cloned)
  • CRISPR knockin positive control when paired with pAAVS1-RFP-DNR (SKU GE100026)
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (7)

The use of this Vectors has been cited in the following citations:
Development of a versatile HPLC-based method to evaluate the activation status of small GTPases ,null, The Journal of Biological Chemistry ,PubMed ID 34801548
Safe harbor-targeted CRISPR-Cas9 homology-independent targeted integration for multimodality reporter gene-based cell tracking ,null, Science Advances ,PubMed ID 33523917
A Safe Harbor-Targeted CRISPR/Cas9 Homology Independent Targeted Integration (HITI) System for Multi-Modality Reporter Gene-Based Cell Tracking ,Kelly, JJ;Saee-Marand, M;Nystrom, NN;Chen, Y;, bioRxiv [pCas-Guide-AAVS1]
Transplantation of hMSCs Genome Edited with LEF1 Improves Cardio-Protective Effects in Myocardial Infarction ,Cho, H;Lee, K;Shen, Y;Shin, T;Ryu, P;Choi, M;Kang, K;Cho, J;, Molecular Therapy - Nucleic Acids ,PubMed ID 32069701 [pCas-Guide-AAVS1]
Ultrasensitive multi-species detection of CRISPR-Cas9 by a portable centrifugal microfluidic platform ,Phaneuf, C;Seamon, K;Eckles, T;Sinha, A;Schoeniger, J;Harmon, B;Meagher, R;Abhyankar, V;Koh, C;, Anal. Methods [pCas-Guide-AAVS1]
Safe Harbor Targeted CRISPR-Cas9 Tools for Molecular-Genetic Imaging of Cells in Living Subjects ,Dubois, V;Zotova, D;Parkins, K;Swick, C;Hamilton, A;Kelly, J;Ronald, J;, The CRISPR Journal [pCas-Guide-AAVS1]
Safe Harbor Targeted CRISPR-Cas9 Tools for Molecular-Genetic Imaging of Cells in Living Subjects. ,null, The CRISPR journal ,PubMed ID 31021241

Documents

Product Manuals
FAQs
SDS
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