pGuide-EF1a-GFP

CAT＃: GE100044

pGuide-EF1a-GFP, gRNA only vector with EF1a driven GFP

Datasheet
SDS
Vector Sequence

CNY 3,710.00

货期*
现货
规格
    • 10 ug
Cited in 1 publication.

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Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
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Specifications

Product Data
Function gRNA only vectors
Features

gRNA only vector (Cas9 not present)

  • gRNA expression under U6 promoter
  • BamH I and BsmB I sites are used for target sequence cloning
  • Expresses gRNA containing the inserted target sequence and the gRNA scaffold
  • GFP as a reporter for tracking or sorting transfected cells
  • CF3 primer (GE100008) can be used to sequence the target sequence after cloning
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this Vectors has been cited in the following citations:
A mutation in SLC37A4 causes a dominantly inherited congenital disorder of glycosylation characterized by liver dysfunction ,Ng, BG;Sosicka, P;Fenaille, F;Harroche, A;Vuillaumier-Barrot, S;Porterfield, M;Xia, ZJ;Wagner, S;Bamshad, MJ;Vergnes-Boiteux, MC;Cholet, S;Dalton, S;Dell, A;Dupré, T;Fiore, M;Haslam, SM;Huguenin, Y;Kumagai, T;Kulik, M;McGoogan, K;Michot, C;Nickerson, DA;Pascreau, T;Borgel, D;Raymond, K;Warad, D;University of Washington Center for Mendelian Genomics (UW-CMG), ;Flanagan-Steet, H;Steet, R;Tiemeyer, M;Seta, N;Bruneel, A;Freeze, HH;, American journal of human genetics ,PubMed ID 33964207 [pGuide-EF1a-GFP]

Documents

Product Manuals
FAQs
SDS
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