pEX-N-His Bacterial Expression Vector

CAT＃: PS100030

pEX-N-His, bacterial expression vector with N-terminal His tag, 10ug

Datasheet
SDS
Vector Sequence

CNY 5,420.00

货期*
现货
规格
    • 10 ug
Cited in 12 publications.

Product images

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Specifications

Product Data
N-Tag His
E. coli Selection Ampicillin (100 ug/mL)
Features
  • Upon IPTG induction, ORFs cloned in this vector can be expressed in E.coli (BL21/DE3) as a tagged protein with an N-terminal His tag.
  • Detection and purification of the transgene are best done using an anti-His antibody or a nickel affinity column.
  • PS100030_F Primer can be used to sequence the target sequence after cloning.
  • PS100028_R Primer can be used to sequence the target sequence after cloning.
Schematic of the multiple cloning sites
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (12)

The use of this Vectors has been cited in the following citations:
Annexin A5 Inhibits Endothelial Inflammation Induced by Lipopolysaccharide-Activated Platelets and Microvesicles via Phosphatidylserine Binding ,null, Pharmaceuticals ,PubMed ID 37375784
Evolution of an Amniote-Specific Mechanism for Modulating Ubiquitin Signaling via Phosphoregulation of the E2 Enzyme UBE2D3 ,Roman-Trufero, M;Ito, CM;Pedebos, C;Magdalou, I;Wang, YF;Karimi, MM;Moyon, B;Webster, Z;di Gregorio, A;Azuara, V;Khalid, S;Speck, C;Rodriguez, T;Dillon, N;, Mol Biol Evol ,PubMed ID 32145025 [pEX-N-His]
IgG-cleaving endopeptidase enables in vivo gene therapy in the presence of anti-AAV neutralizing antibodies ,null, Nature Medicine ,PubMed ID 32483358
Evolution of an amniote-specific mechanism for modulating ubiquitin signalling via phosphoregulation of the E2 enzyme UBE2D3 ,Roman-Trufero, M;Ito, CM;Pedebos, C;Wang, YF;, bioRxiv [pEX-N-His]
Domain analysis of PNKP/XRCC1 interactions: influence of genetic variants of XRCC1 ,Mani, RS;Mermershtain, I;Abdou, I;Fanta, M;Hendzel, MJ;Glover, JNM;Weinfeld, M;, J. Biol. Chem. ,PubMed ID 30446622 [pEX-N-His]
CRMP-1 enhances EVL-mediated actin elongation to build lamellipodia and the actin cortex ,null, The Journal of Cell Biology ,PubMed ID 28630144
Serum neurogranin measurement as a biomarker of acute traumatic brain injury ,null, Clinical biochemistry ,PubMed ID 26025774
The NAP motif of activity-dependent neuroprotective protein (ADNP) regulates dendritic spines through microtubule end binding proteins. ,null, Molecular psychiatry ,PubMed ID 25178163
Investigations into Uncharacterized Radical S-adenosylmethionine Enzymes ,Quintanilla, M;, Thesis [PS100030]
Néstor-Guillermo Progeria Syndrome: a biochemical insight into Barrier-to-Autointegration Factor 1, alanine 12 threonine mutation ,null, BMC Molecular Biology ,PubMed ID 25495845
Bimodal high-affinity association of Brd4 with murine leukemia virus integrase and mononucleosomes ,Larue, RC;Plumb, MR;Crowe, BL;Shkriabai, N;Sharma, A;Difiore, J;Malani, N;Aiyer, SS;Roth, MJ;Bushman, FD;Foster, MP;Kvaratskhelia, M;, Nucleic Acids Res., Feb 2014. ,PubMed ID 24520112 [pEX-N-His]
Identification and Characterization of Citrulline-modified Brain Proteins by Combining HCD and CID Fragmentation ,null, Proteomics ,PubMed ID 23828821

Documents

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TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

    • 1 ml in 1 vial

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CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

    • 5 x 200 ul

CNY 1,280.00 暂停销售

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