Sox15 Mouse siRNA Oligo Duplex (Locus ID 20670)

Specifications

Product Data
Purity	HPLC purified
Quality Control	Tested by ESI-MS
Sequences	Available with shipment
Stability	One year from date of shipment when stored at -20°C.
# of transfections	Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
Note	Single siRNA duplex (10nmol) can be ordered.
Reference Data
RefSeq	NM_009235
Components	Sox15 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 20670) Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol Included - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
Summary	Transcription factor that binds to DNA at the 5'-AACAATG-3' consensus sequence (PubMed:10821863, PubMed:15863505, PubMed:16759287, PubMed:17363903). Acts as a transcriptional activator and repressor (PubMed:10821863, PubMed:15863505, PubMed:16759287). Binds synergistically with POU5F1 (OCT3/4) to gene promoters (PubMed:15863505). Binds to the FOXK1 promoter and recruits FHL3, resulting in transcriptional activation of FOXK1 which leads to myoblast proliferation (PubMed:17363903). Acts as an inhibitor of myoblast differentiation via transcriptional repression which leads to down-regulation of the muscle-specific genes MYOD and MYOG (PubMed:10821863). Involved in trophoblast giant cell differentiation via enhancement of HAND1 transcriptional activity (PubMed:16759287). Regulates transcription of HRC via binding to it proximal enhancer region (PubMed:15863505). Involved in skeletal muscle regeneration (PubMed:15367664, PubMed:17363903). Also plays a role in the development of myogenic precursor cells (PubMed:15367664).[UniProtKB/Swiss-Prot Function]
Performance Guranteed	OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.