TCEB1 Human shRNA Plasmid Kit (Locus ID 6921)

Specifications

Product Data
Product Name	TCEB1 Human shRNA Plasmid Kit (Locus ID 6921)
Locus ID	6921
UniProt ID	Q15369
Synonyms	SIII; TCEB1
Vector	pRS
Format	Retroviral plasmids
Kit Components	ELOC - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 6921). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001204857, NM_001204858, NM_001204859, NM_001204860, NM_001204861, NM_001204862, NM_001204863, NM_001204864, NM_005648, NM_005648.1, NM_005648.2, NM_005648.3, NM_001204863.1, NM_001204864.1, NM_001204857.1, NM_001204858.1, NM_001204859.1, NM_001204860.1, NM_001204861.1, NM_001204862.1, BC013809, BC013809.1, BC093065, BC100028, BC100283, BM720777, BM821650, NM_001204863.2, NM_001204859.2, NM_001204858.2, NM_005648.4
Summary	This gene encodes the protein elongin C, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been identified. [provided by RefSeq, Mar 2011]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.