BLM Human shRNA Plasmid Kit (Locus ID 641)
CAT#: TR314471
BLM - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
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CNY 4,790.00
货期*
现货
规格
Cited in 1 publication. |
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经常一起买 (2)
Specifications
Product Data | |
Product Name | BLM Human shRNA Plasmid Kit (Locus ID 641) |
Locus ID | 641 |
UniProt ID | P54132 |
Synonyms | BS; MGRISCE1; RECQ2; RECQL2; RECQL3 |
Vector | pRS |
Format | Retroviral plasmids |
Kit Components | BLM - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 641). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free. |
RefSeq | NM_000057, NM_001287246, NM_001287247, NM_001287248, NM_000057.1, NM_000057.2, NM_000057.3, NM_001287248.1, NM_001287247.1, NM_001287246.1, BC034480, BC062697, BC093622, BC101567, BC107423, BC115028, BC115029, BC115030, BC115031, BC115032, BC143280, BC143288, NM_001287248.2, NM_001287246.2, NM_000057.4, NM_001287247.2 |
Summary | The Bloom syndrome is an autosomal recessive disorder characterized by growth deficiency, microcephaly and immunodeficiency among others. It is caused by homozygous or compound heterozygous mutation in the gene encoding DNA helicase RecQ protein on chromosome 15q26. This Bloom-associated helicase unwinds a variety of DNA substrates including Holliday junction, and is involved in several pathways contributing to the maintenance of genome stability. Identification of pathogenic Bloom variants is required for heterozygote testing in at-risk families. [provided by RefSeq, May 2020] |
shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
Performance Guaranteed | OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred). |
Citations (1)
The use of this RNAi has been cited in the following citations: |
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ML216 Prevents DNA Damage-Induced Senescence by Modulating DBC1-BLM Interaction.
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Cells
,PubMed ID 36611939
[BLM]
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