CTCF Human shRNA Plasmid Kit (Locus ID 10664)
CAT#: TR313675
CTCF - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
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CNY 4,790.00
货期*
现货
规格
Cited in 1 publication. |
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经常一起买 (2)
Specifications
Product Data | |
Product Name | CTCF Human shRNA Plasmid Kit (Locus ID 10664) |
Locus ID | 10664 |
UniProt ID | P49711 |
Synonyms | CFAP108; FAP108; MRD21 |
Vector | pRS |
Format | Retroviral plasmids |
Kit Components | CTCF - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 10664). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free. |
RefSeq | NM_001191022, NM_006565, NM_006565.1, NM_006565.2, NM_006565.3, NM_001191022.1, BC014267, BC014267.2, NM_001363916, NM_006565.4 |
Summary | This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010] |
shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
Performance Guaranteed | OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred). |
Citations (1)
The use of this RNAi has been cited in the following citations: |
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Identification of CTCF as a master regulator of the clustered protocadherin genes
,Michal Golan-Mashiach, Moshe Grunspan, Rafi Emmanuel, Liron Gibbs-Bar, Rivka Dikstein, and Ehud Shapiro,
Nucleic Acids Res., Dec 2011; 10.1093/nar/gkr1260.
[CTCF]
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