NOTCH3 Human shRNA Plasmid Kit (Locus ID 4854)

CAT#: TR311139

NOTCH3 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided



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CNY 4,790.00


货期*
现货

规格
    • 1 kit

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NOTCH3 rabbit polyclonal antibody
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Specifications

Product Data
Product Name NOTCH3 Human shRNA Plasmid Kit (Locus ID 4854)
Locus ID 4854
UniProt ID Q9UM47
Synonyms CADASIL; CADASIL1; CASIL; IMF2; LMNS
Vector pRS
Format Retroviral plasmids
Kit Components NOTCH3 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 4854). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq NM_000435, NM_000435.1, NM_000435.2, NM_000435.3
Summary This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). [provided by RefSeq, Jul 2008]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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