PIF1 Human shRNA Plasmid Kit (Locus ID 80119)
CAT#: TR306157
PIF1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
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CNY 4,790.00
货期*
现货
规格
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Specifications
Product Data | |
Product Name | PIF1 Human shRNA Plasmid Kit (Locus ID 80119) |
Locus ID | 80119 |
UniProt ID | Q9H611 |
Synonyms | C15orf20; PIF |
Vector | pRS |
Format | Retroviral plasmids |
Kit Components | PIF1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 80119). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free. |
RefSeq | NM_001286496, NM_001286497, NM_001286499, NM_025049, NM_025049.1, NM_025049.2, NM_025049.3, NM_001286499.1, NM_001286496.1, NM_001286497.1, BC018978, BC033254, BC137503, BC137504, NM_001286499.2, NM_001286497.2, NM_025049.4 |
Summary | This gene encodes a DNA-dependent adenosine triphosphate (ATP)-metabolizing enzyme that functions as a 5' to 3' DNA helicase. The encoded protein can resolve G-quadruplex structures and RNA-DNA hybrids at the ends of chromosomes. It also prevents telomere elongation by inhibiting the actions of telomerase. Alternative splicing and the use of alternative start codons results in multiple isoforms that are differentially localized to either the mitochondria or the nucleus. [provided by RefSeq, Nov 2013] |
shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
Performance Guaranteed | OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred). |
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