HMBOX1 Human shRNA Plasmid Kit (Locus ID 79618)

Specifications

Product Data
Product Name	HMBOX1 Human shRNA Plasmid Kit (Locus ID 79618)
Locus ID	79618
UniProt ID	Q6NT76
Synonyms	HNF1LA; HOT1; PBHNF; TAH1
Vector	pRS
Format	Retroviral plasmids
Kit Components	HMBOX1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 79618). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001135726, NM_024567, NM_001324382, NM_001324383, NM_001324384, NM_001324385, NM_001324386, NM_001324387, NM_001324388, NM_001324389, NM_001324390, NM_001324391, NM_001324392, NM_001324393, NM_001324394, NM_001324395, NM_001330498, NR_136757, NR_136758, NM_024567.1, NM_024567.2, NM_024567.3, NM_024567.4, NM_001135726.1, NM_001135726.2, BC069242, BC069242.1, BC009259, NM_001135726.3
Summary	Binds directly to 5'-TTAGGG-3' repeats in telomeric DNA (PubMed:23813958, PubMed:23685356). Associates with the telomerase complex at sites of active telomere processing and positively regulates telomere elongation (PubMed:23685356). Important for TERT binding to chromatin, indicating a role in recruitment of the telomerase complex to telomeres (By similarity). Also plays a role in the alternative lengthening of telomeres (ALT) pathway in telomerase-negative cells where it promotes formation and/or maintenance of ALT-associated promyelocytic leukemia bodies (APBs) (PubMed:23813958). Enhances formation of telomere C-circles in ALT cells, suggesting a possible role in telomere recombination (PubMed:23813958). Might also be involved in the DNA damage response at telomeres (PubMed:23813958).[UniProtKB/Swiss-Prot Function]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.