PIAS2 Human shRNA Plasmid Kit (Locus ID 9063)

Specifications

Product Data
Product Name	PIAS2 Human shRNA Plasmid Kit (Locus ID 9063)
Locus ID	9063
UniProt ID	O75928
Synonyms	ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4
Vector	pRS
Format	Retroviral plasmids
Kit Components	PIAS2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 9063). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	BC015190, NM_004671, NM_173206, NM_001324046, NM_001324047, NM_001324048, NM_001324049, NM_001324051, NM_001324052, NM_001324053, NM_001324054, NM_001324055, NM_001324057, NM_001324058, NM_001324059, NM_001324060, NM_001354033, NM_001354034, NM_001354035, NM_001354036, NM_001354037, NM_001354038, NM_001354039, NR_136684, NR_136685, NR_148699, NR_148700, NR_148701, NR_148702, NR_148703, NM_173206.1, NM_173206.2, NM_173206.3, NM_004671.1, NM_004671.2, NM_004671.3, NM_004671.4, BC015190.1, BC111060, NM_173206.4, NM_004671.5
Summary	This gene encodes a member of the protein inhibitor of activated STAT family, which function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Isoforms of the encoded protein enhance the sumoylation of specific target proteins including the p53 tumor suppressor protein, c-Jun, and the androgen receptor. A pseudogene of this gene is located on the short arm of chromosome 4. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. [provided by RefSeq, Aug 2017]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.