Rprd1b Mouse shRNA Plasmid (Locus ID 70470)

CAT#: TL513161

Rprd1b - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided



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CNY 7,740.00


货期*
2周

规格
    • 1 kit

Product images

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Lenti-vpak packaging kit - packaging plasmids and transfection reagent
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Lateral flow testing sticks used for the semi-quantitative detection of the lentiviral p24 protein, 20 tests
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Specifications

Product Data
Product Name Rprd1b Mouse shRNA Plasmid (Locus ID 70470)
Locus ID 70470
UniProt ID Q9CSU0
Synonyms 2610304G08Rik; 2810446G03Rik; Crept
Vector pGFP-C-shLenti
Format Lentiviral plasmids
Kit Components Rprd1b - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 70470). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq BC028819, BC042447, BC054350, NM_001291134, NM_001291135, NM_001291136, NM_027434, NM_027434.1, NM_027434.2, NM_027434.3, NM_001291136.1, NM_001291135.1, NM_001291134.1
Summary Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation (By similarity).[UniProtKB/Swiss-Prot Function]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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