Pard6a Mouse shRNA Plasmid (Locus ID 56513)

Specifications

Product Data
Product Name	Pard6a Mouse shRNA Plasmid (Locus ID 56513)
Locus ID	56513
UniProt ID	Q9Z101
Synonyms	0710008C04Rik; 2610010A15Rik; Par-6; PAR-6A; Par6; PAR6alpha; Par6c; TAX40; Tip-40
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	Pard6a - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 56513). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	BC049593, BC061114, NM_001047435, NM_001047436, NM_001286344, NM_001286345, NM_019695, NM_001047435.1, NM_001047435.2, NM_019695.1, NM_019695.2, NM_019695.3, NM_001047436.1, NM_001047436.2, NM_001286344.1, NM_001286345.1
Summary	Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins (PubMed:15761148). Regulates centrosome organization and function. Essential for the centrosomal recruitment of key proteins that control centrosomal microtubule organization (By similarity).[UniProtKB/Swiss-Prot Function]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.