Klhl2 Mouse shRNA Plasmid (Locus ID 77113)

Specifications

Product Data
Product Name	Klhl2 Mouse shRNA Plasmid (Locus ID 77113)
Locus ID	77113
UniProt ID	Q8JZP3
Synonyms	6030411N21Rik; ABP-KELCH; AU020744; Mav
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	Klhl2 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 77113). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	BC031142, BC031144, NM_178633, NM_178633.1, NM_178633.2, NM_178633.3, BC024572
Summary	Promotes growth of cell projections in oligodendrocyte precursors. Responsible for degradative ubiquitination of the WNK kinases WNK1, WNK3 and WNK4 (By similarity). Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, such as NPTXR, leading most often to their proteasomal degradation. Plays a role in the reorganization of the actin cytoskeleton.[UniProtKB/Swiss-Prot Function]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.