Cbll1 Mouse shRNA Plasmid (Locus ID 104836)

CAT#: TL510140

Cbll1 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided



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CNY 7,740.00


货期*
2周

规格
    • 1 kit

Product images

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Lenti-vpak packaging kit - packaging plasmids and transfection reagent
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TurboFectin Transfection Reagent (1 mL in 1 vial)
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Cbll1 Antibody - C-terminal region
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Lateral flow testing sticks used for the semi-quantitative detection of the lentiviral p24 protein, 20 tests
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Specifications

Product Data
Product Name Cbll1 Mouse shRNA Plasmid (Locus ID 104836)
Locus ID 104836
UniProt ID Q9JIY2
Synonyms AI467391; c-Cbl-like; Hakai
Vector pGFP-C-shLenti
Format Lentiviral plasmids
Kit Components Cbll1 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 104836). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq BC019529, NM_001253847, NM_001253848, NM_134048, NM_134048.1, NM_134048.2, NM_001253848.1, NM_001253847.1
Summary E3 ubiquitin-protein ligase that mediates ubiquitination of several tyrosine-phosphorylated Src substrates, including CDH1, CTTN and DOK1 (PubMed:11836526, PubMed:22252131). Targets CDH1 for endocytosis and degradation (PubMed:11836526). Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29535189, PubMed:29547716). Its function in the WMM complex is unknown (PubMed:29535189, PubMed:29547716).[UniProtKB/Swiss-Prot Function]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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